1993 Fiscal Year Final Research Report Summary
Serum Cytotoxic Factor and its' Inhibitorin Rheumatoid Arthritis.
| Project/Area Number |
04807106
|
|---|
| Research Category |
Grant-in-Aid for General Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Orthopaedic surgery
|
|---|
| Research Institution | Osaka University |
|---|
Principal Investigator |
OCHI Takahiro Osaka University Environmental Medicine Professor, 医学部, 教授 (80112035)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
KIMURA Tomoatsu Osaka University Orthpaedics Assistant Professor, 医学部, 講師 (80167379)
ONO Keiro Osaka University Orthpaedics Professor, 医学部, 教授 (70028330)
|
|---|
| Project Period (FY) |
1992 – 1993
|
| Keywords | Reumatoid arthritis / Serum / Citotoxity / Inhibitor |
|---|
| Research Abstract |
The peripheral blood serum of patients with severe active rheumatoid arthritis (RA) was found to have a cytotoxic effect on autologous mononuclear cells. The cytotoxic factor was partially purified and shown to have a molecular weight of about 900 kd, using gel filtration. The factor differed from cytokines such as tumor necrosis factor-alpha (TNF-alpha) and also differed from complement components. It also differed from hydrolytic enzyme such as protease, collagenase, and phospholipase A_2. It destroyed normal peripheral blood mononuclear cells in vitro at the same concentration as it found in vivo, and also nonspecifically destroyed such human cell lines as CCRF-CEM, Raji, U-937, and HL-60. Each RA patient with this serum cytotoxic factor had severe systemic tissue disease as well as rapid and severe destruction of most joints. Thus, we have identified a cytotoxic factor in RA serum which causes systemic tissue damage. And we also partially pirified the inhibitor against the cytotoxic factor, using gel filtration.
|
-
-
-
-
-
-
-
[Publications] Ochi T, Hakomori S, Fujimoto M, Okamura M, Owaki H, Wakitani S, Shimaoka Y, Hayashida K, Tomita K, Kawamura S, Ono K.: "Therapeutic Effect of Intradermal Injections with Difucosyl Lactosamine(Dimeric Lex)on Patients with Rheumatoide Arthritis." The Journal of Rheumatology. 20. 2038-2045 (1993)
Description
「研究成果報告書概要(欧文)」より
-
-
