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1993 Fiscal Year Final Research Report Summary

New drug delivery system for cancer chemotherapy using lipid commpositions characteristic of cancer cell membrane

Research Project

Project/Area Number 04807145
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 外科・放射線系歯学
Research InstitutionHIROSHIMA UNIVERSITY

Principal Investigator

OKAMOTO Tetsuji  Hiroshima Univ., Scho. of Dent., Assistant Prof., 歯学部・附属病院, 講師 (00169153)

Co-Investigator(Kenkyū-buntansha) TAKADA Kazuaki  Hiroshima Univ., Scho. of Dent. Prof., 歯学部, 教授 (30029970)
Project Period (FY) 1992 – 1993
KeywordsSquamous carcinoma / Adenocarcinoma / Anti cancer drug / Lipid composition / Photodynamic therapy / Liposome / drug delivery system / Serum-free culture
Research Abstract

It has been known that the effects of anti cancer drug is dependent of histological typing of the target cancer cells. We have speculated that the heterogeneity of the frug effect is resulted from the defference of membrane hydrophobicity of the target cancer cells. One of major factors determine membrace hydrophobicity membrane lipid compositions.
On the first research year, we have studied lipid metabolism and lipid compositions of several oral squamous cell carcinoma cells(SCC) and salibary gland derived adenocarcinoma cells(SAC) in serum-free cell culture. We have found that 80% of total SCC membrane lipid is phosphokipid and 20% is free cholesterol. On the other hand, 80% of total SAC membrane lipid is meutral lipid such as trigriceride and eaterified cholesterol, and 20% is phospholipid, these results apparently revealed that the lipid composition of SCC membrane is defferent from that of SAC and suggest that hydrophobicith of SCC membrane is higher than that of SAC membrane.
On the second research year, we have studied the effects of bleomycin (BLM), peplomycin(PLM), cis-platinum(CDDP) and carboplatin(CDBCA) on the growth of weveral SCC and SAC in werum-free cell culture, the cytotoxic activities of BLM and PLM on SCC are stronger than these on SAC.On the other hand, the activities of CDDp and CDBCA on SAC are stronger than these on SCC.Furthermore, these activities are dependent on the intra-cellular concentration of the drugs. These results strongly suggest that cytotoxic effect of anticancer drug are dependent on the hydrophobicity of the cancer cell membrane. We are currently planning to study the effect of lipisome that is complexed anticancer drug with membrane lipid which is the same as lipid composition of target cell membrane.

  • Research Products

    (16 results)

All Other

All Publications (16 results)

  • [Publications] Y.Fujita: "A Novel Heparin-Binding Protein,HBp15 is Identified As Mammalian Ribosomal Protein L22" Biochem.Biophys.Res.Commun.(印刷中). (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 岡本哲治: "雄性マウス顕下腺におけるFGF-1の機能とテストステロン依存性" 日本口腔組織培養研究会誌. Vol.3. 37-38 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 虎谷茂昭: "新しい光感受性色素(フェオフォバイド-α)を用いた口腔癌に対する光力学療法の基礎的研究" 日本口腔外科学会誌. 38. 729-736 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 新木恒猪: "無血清培養下における正常上皮細胞ならびに癌細胞の脂質代謝に及ぼす細胞増殖因子の影響" 日本口腔外科学会誌. 38. 424-432 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 新木恒猪: "無血清培養下における正常上皮細胞唾液腺由来上皮細胞ならびに口腔癌細胞の脂質代謝" 日本口腔外科学会誌. 38. 222-228 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 岡本哲治: "細胞成長因子part III(Vascular Endothelial growth factor)" 日本組織培養学会編(印刷中), (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] J.D.Sato: "Cell Culture Methods For Cell Biology" ARL Press., 136 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] J.D.Sato: "Basic Animal Cell Culture:A practical approach" Oxford.Univ.Press., 340 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y.Fujita, T.Okamoto, et al: "Anovel heparin-binding protein, HBp15 is indentified as mammalian rebosomal protein L22." Biochem. Biophys. Res. Commun. (in press). (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Okamoto, Y.Fujita, et al: "Function and testosterone dependency of FGF-1 in male mouse submaxillary gland." Journal of Japanese Association of Oral Biology. Vol.3. 37-38 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S.Toratani, T.Okamoto, et al: "A study of photodynamic therapy against human oral cancer cells using a new photosensitizwe(pheophorbide-a)" Jpn. J.Oral Maxillofac. Surg.Vol.38. 729-736 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Shinki, T.Okamoto, et al: "Effects of growth factors of the lipid metabolism of normal ephithelial cells and cancer cells in serum-free culture" Jpn. J.Oral Maxillofac. Surg.Vol.38. 424-432 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Shinki, T.Okamoto, et al: "Lipid metabolisms of normal epithelial cells and cancer cells derived from oral region in serum-free culture" Jpn. J.Oral Maxillofac. Surg.Vol.38. 222-228 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Okamoto, Y.Myoken: "Peptide growth factors : in vitro to in vivo" Japanese Tissue Clture Association. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] J.D.Sato, T.Okamoto, et al: Cell Cutrure Methods For Cell Biology. ARL Press, (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] J.D.Sato, T.Okamoto, et al: Basic Animal Cell Cutrure : A prectical approach. Oxford Univ. Press, (1994)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1995-03-27  

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