1993 Fiscal Year Final Research Report Summary
Structural Study on th Carbohydrate-Protein Linkage Region of Proteglycans
| Project/Area Number |
04807162
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Biological pharmacy
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| Research Institution | Kobe Women's College of Pharmacy |
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Principal Investigator |
SUGAHARA Kazuyuki Kobe Women's Coll.of Pharmacy, Dept.of Physiol.Chem., Professor, 薬学部, 教授 (60154449)
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| Co-Investigator(Kenkyū-buntansha) |
YAMADA Shuhei Kobe Women's Coll.of Pharmacy, Dept.of Physiol.Chem., Instructor, 薬学部, 助手 (70240017)
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| Project Period (FY) |
1992 – 1993
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| Keywords | Heparin / Chondroitin sulfate / Glycosaminoglycans / Galactose / Sulfation |
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| Research Abstract |
It has become evident that the sulfated glycosaminoglycans exert various biological activities. However, their structure and biosynthetic mechanisms are not fully understood. In this study we carried out structural studies on the carbohydrate-protein linkage region of various sulfated glycosaminoglycans including chondroitin 6-sulfate ad heparin. Although the former is galactosaminoglycans and the latter is glucosaminoglycans, they share the common tetrasaccharide core structure in the carbohydrate-protein linkage region.
We discovered hitherto unreported 4-0-sulfated and 6-0-sulfated galactose-containing structures in the carbohydrate-protein linkage region of chondroitin 6-sulfate prepared from shark cartilage. These strucures were Galbeta1-3Gal(6-0-sulfate)beta1-4Xyl, Gal(6-0-sulfate)beta1-3Gal(6-0-sulfate)beta1-4Xyl and Gal(4-0-sulfate)beta1-3Gal(6-0-sulfate)beta1-4Xyl. In contrast, these structures were not detected in the corresponding region of heparin prepared from porcine intes
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tine. Thus, this study has clarified that there are structural differences in the carbohydrate-protein linkage region of chondroitin sulfate and heparin. Based upon these results, we propose a working hypothesis that the sulfation of the galactose residues in the linkage region may be involved in the biosynthetic sorting mechanisms of chondroitin sulfate and heparin. It is possible that tha GalNAc-transferase which transfers the first GalNAc residue to the linkage tetrasaccharide, GlcAbeta1-3Galbeta1-3Galbeta1-4Xyl, recognaizes the sulfated galactose structures while the GlcNAc-transferase which transfers the first GlcNAc residue to the linkage tetrasaccharide is inhibited by these modified structures. This study also revealed that there are a number of subclasses in the chondroitin sulfate chains based upon the structural differences in the carbohydrate protein linkage region. In the future study we plan to investigate the structural relationship between the linkage region and the repeat region where biologically active domain structures are located. Less
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[Publications] Sugahara, K., Yamada, S., Sugiura, M., Takeda, K., Yuen, R., Khoo, H.E.and Poh, C.H.: "Identification of the Reaction Products of the Purified Hyaluronidase from Stonefish(Synanceja horrida) Venom" Biochem.J.283(1). 99-104 (1992)
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「研究成果報告書概要(欧文)」より
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[Publications] Yamada, S., Yoshida, K., Sugiura, M., Sugahara, K., Khoo, K.H., Morris, H.R., and Dell, A.: "Structural Studies on the Bacterial Lyase-resistant Tetrasaccharides Derived from the Antithrombin III-binding Site of Porcine Intestinal Heparin" J.Biol.Chem.268(7). 4780-4787 (1993)
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「研究成果報告書概要(欧文)」より
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[Publications] Yamada, S., Sakamoto, K., Tsuda H., Yoshida, K, Sugahara, K., Khoo, K.H., Moriis, H.R., and Dell, A.: "Structural Studies on th Tri-and Tetrasaccharides Isolated from Porcine Intestinal Heparin and Characterization of Heparinase/Heparitinases Using Them as Substrates" Glycobiology. 4(1). 69-78 (1994)
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「研究成果報告書概要(欧文)」より
