1993 Fiscal Year Final Research Report Summary
Reactivity of anti-carbohydrate monoclonal antibodies with normal blood cells and leukemic cells and clinical application of the antibodies
| Project/Area Number |
04807178
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Hematology
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| Research Institution | Jichi Medical School |
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Principal Investigator |
MUROI Kazuo 自治医科大学, 医学部, 講師 (50190939)
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| Project Period (FY) |
1992 – 1993
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| Keywords | carbohydrate / CD34 / leukocyte / lymphocyte / erythrocyte / autologous bone marrow transplantation |
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| Research Abstract |
1. sialosyl-Lewis^x
We examined expression of sialosyl-Lewis^x in blood cells. Sialosyl-Lewis^x was expressed in granulocytes, monocytes and granulocyte-monocyte precursors but not in normal CD34-positive cells. In lymphoid cells, sialosyl-Lewis^x was transiently expressed in CD10-positive cells of B-cell precursors. Since sialosyl-Lewis^x was highly expressed in CD34-positive leukemic cells, the leukemic cells were suggested to over-express CD34. Accordingly, for autologous bone marrow transplantation, anti-sialosyl-Lewis^x antibody was suggested to be useful for eleminating reidual leukemic cells from bone marrow cells in leukemia patients in complete remission.
2. sialosyl-Tn
We examined expession of sialosyl-Tn which was one of the criptic antigens of glycophorin A.Sialosyl-Tn was expressed in CFU-E to erythroblasts but not in normal CD34-positive cells and BFU-E.In leukemic cells, sialosyl-Tn was highly expressed in erythroleukemia cells (M6). In lymphoid cells, sialosyl-Tn was expressed in mature B-cells and a subset of T-cells. Since sialosyl-Tn was not expressed in CD10-positive cells of B-cell precursors and acute lymphoblastic leukemia cells, the antigen was suggested to be only expressed in mature lymphocytes. Although the nature of sialosyl-Tn was unclear, expression pattern of sialosyl-Tn was similar to that of CD22.
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