1993 Fiscal Year Final Research Report Summary
Analysis of the formation mechanism of fertilization membarane on see urchin eggs using wasp venom peptide analogs
| Project/Area Number |
04808022
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
物質生物化学
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| Research Institution | Suntory Institude for Bioorganic Research (1993)
The University of Tokyo (1992) |
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Principal Investigator |
SAITO Kazuki Suntory Institute for Bioorganic Research, Researcher, 研究員 (10192585)
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| Project Period (FY) |
1992 – 1993
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| Keywords | Wasp Venom Pepides / Sea Urchin Eggs(Hemicentroutus pulcherrimus) / Elevation of Fetilization Membrane / Contical Reactions / Aggregation of Chromosomes / Formation of Actin Bundle |
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| Research Abstract |
Forty years ago, crude wasp venom from Polistes jadwigae was found to elevate the fertilization membrane(FM)of sea urchin eggs, but effective components of the venom have not been elucidated yet. Mastoparan, a peptide in the wasp venom, was thought to be the first candidate for such an effective component, because it was known to be able to activate G protein, which is a major transducer of biosignals in cells.
1. The activity of various natural wasp peptides to elevate the FM of sea urchin eggs was examined. Mastoparan was foudnd to elevate the FM in part, but it cannot be the effective component of the venom, because other wasp peptides, such as melittin and chemotactic peptides, could act to the eggs more effectively.
2. While mastoparan itself hardly elevates the FM of sea urchin eggs, it was found to obtain the activity by modification of N^<alpha>-or N^<epsilon>-amino groups. Among the analogs, [Lys(Dns)^<11>]mastoparan was most effective, but truncated derivatives of the dansylated peptide that lacked a few N-terminal residuees did not show any activity.
3. From various indirect evidences, [Lys(Dns)^<11>]mastoparan was not thought to activate the G protein to elevate the FM of sea urchin eggs. The peptide must promote only the topical cortical reactions, but it was also found to induce the aggregation of chromosomes and the formation of actin bundle. This indicates that the dansylaled mastoparan acts to the cells not as a non-specifiic detergent but as a promoter of the cortical reactions and the initial segmentation.
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