Mass spectrometric analysis of crosslinker of aging proteins formed by Maillard reaction

1993 Fiscal Year Final Research Report Summary

• Project/Area Number: 04836009
• Research Category: Grant-in-Aid for General Scientific Research (C)
• Allocation Type: Single-year Grants
• Research Field: 老化(加齢)
• Research Institution: Nagoya University
• Principal Investigator: NIWA Toshimitsu Nagoya University, School of Medicine, Associate Professor, 医学部, 助教授 (20208268)
• Co-Investigator(Kenkyū-buntansha): NIWA Toshimitsu Nagoya University, School of Medicine, Associate Professor (20208268) ; NIWA Toshimitsu Nagoya University, School of Medicine, Associate Professor (20208268) ; NIWA Toshimitsu Nagoya University, School of Medicine, Associate Professor (20208268) ; NIWA Toshimitsu Nagoya University, School of Medicine, Associate Professor (20208268) ; NIWA Toshimitsu Nagoya University, School of Medicine, Associate Professor (20208268)
• Project Period (FY): 1992 – 1993
• Keywords: Maillard reaction / 3-deoxyglucosone / gas chromatography / mass spectrometry / diabetes mellitus / diabetic nephropathy

Research Abstract

3-Deoxyglucosone, a potent protein crosslinking intermediate of the Maillard reaction, was first detected in diabetic serum using gas chromatography/mass spectrometry. Serum concentration of 3-deoxyglucosone was elevated in diabetic patients as compared with healthy subjects. More notably, diabetic patients with nephropathy showed higher serum concentration of 3-deoxyglucosone than those without nephropathy. The increased serum concentration of 3-deoxyglucosone in the diabetic patients suggests that 3-deoxyglucosone may be responsible for the development of diabetic comlications such as diabetic nephropathy by promoting the formation of advanced glycation end products.

Research Products

• [Publications] Toshimitsu NIWA: "Presence of 3-deoxyglucosone,a potent protein crosslinking intermediate of Maillard Reaction,in diabetic serum." Biochem Biophys Res Commun. 196. 837-843 (1993)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Niwa, T., Takeda, N., Yoshizumi, H., Tatematsu, A., Ohara, M., Tomiyama, S., Niimura, K.: "Presence of 3-deoxyglucosone, a potent protein crosslinking intermediate of Maillard Reaction, in diabetic serum" Biochem. Biophys. Res. Commun. 196. 837-843 (1993)
  • Description: 「研究成果報告書概要(欧文)」より