1993 Fiscal Year Final Research Report Summary
Genetic Analysis and Expression of Senescence Marker PROTEIN-30 (SMP30) Decreasing Androgenindependenty with AGE in the Rat Liver.
| Project/Area Number |
04836027
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
老化(加齢)
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| Research Institution | Tokyo Metropolitan Institu t of Gerontology |
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Principal Investigator |
FUJITA Toshiko TMIG, Mol. Biology, Assistant Researcher, 分子生物学, 助手 (00100131)
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| Co-Investigator(Kenkyū-buntansha) |
MARUYAMA Naoki T,OG, Mol. Pathoogy, Head, 分子病理, 室長 (00115940)
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| Project Period (FY) |
1992 – 1993
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| Keywords | Aging / Senescence / Senescence Marker Protein-30 / SMP30 / Regicalucin / Calucium-Binding Protein / Liver / Kidney / cDNA |
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| Research Abstract |
We discovered a novel rat liver protein, the amount of which decreased androgen-independently with aging. We designated this protein, whose molecular mass was 30 KDa and pI vaue was 4.9, as senescence marker protein -30 (SMP30). The localization of SMP30 was restricted to liver and Kidney among numerous organs tested. We have isolated and characterized two cDNA clones, one consisted of 1588-bp nucleotides and other of 1195-bp nucleotides generated by alternative polyadenylation. Northern hybridization analysis showed two species of mRNA (1.7-kb and 1.4-kb) in the liver and kidney. The open reading frame encodes 299 amino acids. SMP30 is widely conserved among higher animals.
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