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1995 Fiscal Year Final Research Report Summary

Isolation of AIDS-related viruses from African Primates

Research Project

Project/Area Number 05041111
Research Category

Grant-in-Aid for international Scientific Research

Allocation TypeSingle-year Grants
SectionField Research
Research InstitutionKYOTO UNIVERSITY

Principal Investigator

HAYAMI Masanori  Professor, Institute for Virus Research, Kyoto University, ウイルス研究所, 教授 (40072946)

Co-Investigator(Kenkyū-buntansha) IDO Eiji  Instructor, Institute for Virus Research, Kyoto University, ウイルス研究所, 助手 (70183176)
MIURA Tomoyuki  Instructor, Institute for Virus Research, Kyoto University, ウイウス研究所, 助手 (40202337)
Project Period (FY) 1993 – 1995
KeywordsAIDS / Africa / HIV / Cameroon / Double infection / recombination
Research Abstract

1.Since the discovery of HIV-1 and HIV-2 as causative agents of AIDS,efforts have been directed towards understanding the origins and evolutionary history of HIV.HIV-1 and HIV-2 are closely related to primate lentiviruses (SIV). In this project, we have studied the diversity of related nonhuman primate lentiviruses and speculated on the origins of HIV in Africa. In this 3 years, we concentrated to clarify the molecular epidemiology of HIV in Cameroon and to assess the incidence of mixed-infection and recombination.
2.Forty eight HIV (47 HIV-1, one HIV-2) were obtained from Cameroonian AC,ARC and AIDS patients in 1994 and 1995. Part of the pol region suitable for comparing all the groups of HIV-related lentiviruses and part of the HIV-1 env region including the V3 loop were amplified by nested PCR,sequenced, and phylogenetically analyzed.
3.The majority of Cameroonian HIV-1s belonged to clade A (32), but the rest belonged to various clades : clade B (2), clade C (2), Clade D (2), clade E (1), Clade F (4) and group O (4). In the pol analysis, two different types were found in four individuals (HIV-2a and HIV-1 clade A,HIV-1 group O and clade A,clades A and C,and clades C and F), suggesting double infections with different HIV-1 subtypes or groups.
4.Various mixed-infection such as between different clades of HIV-1 group M,between HIV-1 and HIV-2, and even between HIV-1 groups O and M were confirmed at a rather high frequency (around 10%) in the analyzed specimens. Some of the data suggested recombination between different clades of HIV-1 group M were also obtained. Thus phylogenetic analysis based on the pol and env regions provided a new insight to the understanding of HIV phylogeny.

  • Research Products

    (6 results)

All Other

All Publications (6 results)

  • [Publications] 奥村恭司、他。: "Enzygnost Anti HIV1/2 PlusによるHIV-1(サブタイプOを含む)抗体およびHIV-2抗体の検出" 医学と薬学. 33. 741-751 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yamashita,M.,et al.: "Presence of the widespread sudtype of HTLV-I in South Africa" AIDS & Human Retroviruses. 11. 645-647 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kuwata,T.,et al.: "Construction of human immunodeficiency virus 1/simian immunodeficiency virus sStrain mac chimeric viruses having vpr and/or nef of different parental origins and their in vitro and invivo replication" J.Gen.Virol.76. 2181-2191 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 奥村恭司、他。: "ジェラネビアミックスによるHIV-1抗体およびHIV-2抗体の検出" 医学と薬学. 34. 867-876 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Chen,J.,et al.: "HTLV-I isolated from a pygmy in Camerosn is related to but distinct from the known central African type." AIDS & Human Retroviruses. 11. 1529-1531 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 速水正憲: "サルからみた、エイズ、HIVは何処から来て何処へ行くのか" モダンメディア. 41. 44-51 (1995)

    • Description
      「研究成果報告書概要(和文)」より

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Published: 1997-03-04  

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