| Project/Area Number |
05044029
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| Research Category |
Grant-in-Aid for international Scientific Research
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| Allocation Type | Single-year Grants |
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| Section | Joint Research |
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| Research Institution | Tohoku University |
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Principal Investigator |
OGURA Kyozo Institute for Chemical Reaction Science, Tohoku Univ., Japan, 反応化学研究所, 教授 (80006303)
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| Co-Investigator(Kenkyū-buntansha) |
TANAKA Yasuyuki Dept.of Biomaterial Engineering, Faculty of Technology, Tokyo University of Agri, 工学部, 教授 (80015114)
WITITSUWANNA デー. Mahidol University, Faculty of Science, 助教授
KOYAMA Tanetoshi Dept.of Biochemistry and Engineering, Faculty of Engineering, Tohoku Univ., Japa, 工学部, 助教授 (20089808)
WITITSUWANNAKUL Dhirayos Faculty of Science, Mahidol Univ., Thailand
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| Project Period (FY) |
1993 – 1994
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| Keywords | Prenyltransferase / Natural Rubber / Farnesyl Diphosphate / Isopentenyl Diphosphate / Latex / Isomerase / Synthase / Isoprene Chain Elongation |
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| Research Abstract |
Isopentenyl diphosphate (IPP) isomerase [EC 5.3.3.2], which catalyzes the reversible isomerization of IPP to dimethylallyl diphosphate, is presumed to be involved in rubber biosynthesis, but no direct evidence has been given for its occurrence in rubber latex. This enzyme activity was found in the C-serum powder prepared by lyophilization of centrifuged rubber latex of Hevea brasiliensis.
In connection with the biosynthesis of rubber, prenyltransferase activities in the C-serum powder were examined. Farnesyl diphosphate (C_<15>) and geranylgeranyl diphosphate (C_<20>) were detected as major products. In addition, a polyprenyl diphosphate (-C_<30>) was obtained when geranyl diphosphate (C_<10>) was employed as the allylic substrate. When the C-serum was chromatographed on DEAE-Toyopearl, several prenyltransferase fractions were obtained. One of the partially purified enzyme fractions showed an activity of producing a polyprenyl product with a chain length longer than C_<80> as well as a C_<15> product.
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