1995 Fiscal Year Final Research Report Summary
Interaction in plasmodesmata between plant viruses
| Project/Area Number |
05044140
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| Research Category |
Grant-in-Aid for international Scientific Research
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| Allocation Type | Single-year Grants |
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| Section | Joint Research |
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| Research Institution | Teikyo University |
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Principal Investigator |
WATANABE Yuichiro Teikyo Univ.School of Science and Engineering, Associate Professor, 理工学部, 助教授 (60183125)
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| Co-Investigator(Kenkyū-buntansha) |
BEACHY Roger the Scripps Research Institute, Dept.Cell Biology, 植物生物学部, 部長
HOSOKAWA Daijiro Tokyo Univ.of Agriculture and Engineering, 農学部, 教授 (50014957)
OKADA Yoshimi Teikyo Univ.School of Science and Engineering, 理工学部, 教授 (30011703)
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| Project Period (FY) |
1993 – 1995
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| Keywords | plant virus / plant / plasmodesmata / movement protein / phosphorylation / virus resistance |
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| Research Abstract |
We have done several projects by this collaboration. 1) Analysis of tobamovirus Ob which can overcome the tobacco N gene action. The action is known to inhibit tobamovirus spread by formation of local necrotic lesion (NLL) at the virus entry region. New variants originated from Ob formed NLLs on tobacco plants even with N gene. The molecular recombination techniques revealed that replicase region of Ob decide whether or not a specific strain cause NLLs. 2) Utilization of green fluorescent protein (GFP) as a molecular tag to chase the spread of tabamovirus. GFP is now known as a useful tool to visualize a specific molecule inside the cell at the living condition. We substituted coat protein gene by GFP gene, giving rise to a new tobamovirus which emit GFP fluorescence after spread and illumination by UV.This strategy enabled us several projects with various tobamoviruses. 3) TMV-R is described as a new tobamovirus which can infect rakkyo (a kind of shallot), monocot crop, while common strain of TMV cannot. Molecular recombination studies with GFP tags showed us that replicase regions of each strain dictated the preference of host range. 4) The GFP gene was inserted just after movement gene (MP) in the virus construct to produce fusion protein MP : GFP.This new virus can replicate and spread in planta nearly as efficiently as wild type virus. GFP tag visualized us the location where MP is being synthesized, how late the virus moves, what kind of subcellular locations MP is showing in plants. We are now observing time-course change of MP localization using a fluorescent microscopy. 5) We have mapped the phosphorylation sites of ToMV movement protein. There were two target serine sites, one of which phosphorylation is critical for function. Substitution of serine into alanine resulted in non-viable virus as well as no phosphorylation. The localization of mutant MP : GFP was different from that of wild type MP : GFP.Still in progress.
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Research Products
(12 results)
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[Publications] Chng, G., Wong, S.M., Loh, C.S., Goh, C.J., Khiaw, H., Chung, M., Watanabe, Y.: "The Complete nacleotide sequence of a Singapore isolate of ORSV and its comparison with 8ther tobamoviruses" Gene. (in press). (1996)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Watanabe, Y., Ogawa, T., Takahashi, H., Ishida, I., Takeuchi, Y., Yamamoto, Y., Okada, Y.: "Resistance against multiple plant viruses in plants mediated by a ds-RNA specific ribonuclease" FEBS Lett.372. 165-168 (1995)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Padgett, H., Epel, B., Kahn, T., Heinlein, M., Watanabe, Y., Beachy, R.: "Subcellular distribation of tobamovirus movement protein in infected leaves and implications for cell-to-cell spread of infection" Plant J.(in press). (1996)
Description
「研究成果報告書概要(欧文)」より
