1993 Fiscal Year Annual Research Report

がん研究に要する研究資材開発

Research Project

Project/Area Number	05151017
Research Institution	The University of Tokyo
Principal Investigator	黒木登志夫東京大学, 医科学研究所, 教授 (90006073)
Co-Investigator(Kenkyū-buntansha)	渋谷正史東京大学, 医科学研究所, 教授 (10107427) 橋本嘉幸東北大学, 薬学部, 教授 (90072412) 吉田迪弘北海道大学, 理学部, 教授 (60001765) 青木國雄愛知がんセンター, 総長 (60073102) 豊島久真男大阪大学, 微生物病研究所, 教授 (90029760)
Keywords	遺伝情報 / がん遺伝子 / 単クローン抗体 / 染色体マッピング / 化学発がん物質 / コホート集団 / 移植腫瘍 / ペプチド合成
Research Abstract	がん研究のためには各種の研究資材が必要である。本研究班はがん研究に必要な資材を調整し、分与し、あるいはそれらの所在情報を、がん研究者に広く供給することを目的としている。具体的には次の8つの事業を行った。(平成5年1-12月期の実績を示す。) 1.移植腫瘍・細胞の分与(工藤俊雄):358株を供給した。移植腫瘍の所在情報を平成5年6月刊行した。 2.化学物質(黒木登志夫):IQ,MeIQ,NitroIQの供給はなかった。 3.単クローン抗体(橋本嘉幸、高橋利忠):本年度は所在情報パンフレットを作成しなかった。 4.遺伝子(豊島久真男、渋谷正史、山本雅):プローブ3件配布した。ヒト・マウスハイブリトーマDNA1件配布した。 5.染色体マッピング(吉田迪弘、中村祐輔):マッピング用ハイブリドーマ細胞4件。in situ hybridization法によるマッピング18件およびマッピング用プローブ200件分与した。予研遺伝子バンクに染色体クローン985件登録した。 6.遺伝情報(宮田隆、五條堀孝、伊藤彬):遺伝子のコンピューター解析26件、VAXおよび後継機種AXPによる分析3341件、および超遺伝子族データベースのパンフレットを作成した。 7.DNA保存(黒木登志夫、青木國雄):コホートの末梢血DNAを100μgずつ約1000件収集した。 8.ペプチド合成(富田基郎、吉田光昭、黒木登志夫):班員の要望に応じて56件のペプチドを合成した。

Research Products

(13 results)

All Other

All Publications (13 results)

[Publications] Osada,M.: "Predominant expression of nPKC〓,a Ca^<2+>-independent isoform of protein kinase C in epithelial tissues,in association with epithelial differentiation." Cell Growth & Differentiation. 4. 167-175 (1993)
[Publications] Kudo,T.: "A novel human monoclonal antibody directed to a tumor-associated antigen." Jpn.J.Cancer Res.84. 760-769 (1993)
[Publications] Emi,N.: "Isolation of a novel cDNA showing marked similarity to ME491/CD63 superfamily." Immunogenetics. 37. 193-198 (1993)
[Publications] Asano,H.: "The mouse Mel-18 “RING-finger" gene:genomic organization,promoter analysis and chromosomal assignmtnt." DNA Sequence-. 3. 369-377 (1993)
[Publications] Nakamura,Y.: "Isolation and mapping of 328 new cosmid markers on human chromosome 8:construction of a high-resolution cytogenetic map of chromosome 8 with 416 markers." Cytogenetics and Cell Genetics. 65. 115-118 (1994)
[Publications] Hamada,F.: "Association of immunoglobulin G Fc recptor II with Src-like protein-tyrosine kinase Fgr in neutrophils." Proc.Natl.Acad.Sci.USA. 90. 6305-6309 (1993)
[Publications] Gojobori,T.: "Genetic variability of major histocompatibility complex in human populations." Human Population Genetics(ed.by P.P.Majumder)Plenum Press,New York. 67-79 (1993)
[Publications] Jinno,A.: "Testis-specific Mak protein kinase is expressed specifically in the meiotic phase in suspermatogenesis and is associated with a 210-kilodalton cellular phosphoprotein." Mol.Cell.Biology. 13. 4146-4156 (1993)
[Publications] Shirasawa,S.: "Alered growth of human colon cancer cell lines disrupted at activated Ki-ras." Science. 260. 85-88 (1993)
[Publications] Kuma,K.: "Motifs of cadherin-and fibronectin Type III-related sequences and evolution of the receptor-Type-protein tyrosine kinases:Sequence similarity between proto-oncogene ret and cadherin family." Mol.Biol.Evol.10. 539-551 (1993)
[Publications] Sugita,Y.: "Determination of a carboxyl-terminal residue and disulfide bonds of MACIF(CD59),a glycosyl-phosphatidyl inositol-anchored" J.Biochem.114. 473-477 (1993)
[Publications] Pinak,M.: "Energy deposition in structural parts of DNA by monoenergetic electrons." J.Radiat.Res.34. 221-234 (1993)
[Publications] Suzuki,T.: "The trans-activator tax of human T-cell leukemia virus type1(HTLV-1)interacts with cAMP-responsive element(CRE)binding and CRE modulator proteins that bind to the 21-base-pair enhancer of HTLV-1." Proc.Natl.Acad.Sci.USA. 90. 610-614 (1993)

1993 Fiscal Year Annual Research Report

がん研究に要する研究資材開発

Principal Investigator

黒木 登志夫 東京大学, 医科学研究所, 教授 (90006073)

Research Products

[Publications] Osada,M.: "Predominant expression of nPKC〓,a Ca^<2+>-independent isoform of protein kinase C in epithelial tissues,in association with epithelial differentiation." Cell Growth & Differentiation. 4. 167-175 (1993)

[Publications] Kudo,T.: "A novel human monoclonal antibody directed to a tumor-associated antigen." Jpn.J.Cancer Res.84. 760-769 (1993)

[Publications] Emi,N.: "Isolation of a novel cDNA showing marked similarity to ME491/CD63 superfamily." Immunogenetics. 37. 193-198 (1993)

[Publications] Asano,H.: "The mouse Mel-18 “RING-finger" gene:genomic organization,promoter analysis and chromosomal assignmtnt." DNA Sequence-. 3. 369-377 (1993)

[Publications] Nakamura,Y.: "Isolation and mapping of 328 new cosmid markers on human chromosome 8:construction of a high-resolution cytogenetic map of chromosome 8 with 416 markers." Cytogenetics and Cell Genetics. 65. 115-118 (1994)

[Publications] Hamada,F.: "Association of immunoglobulin G Fc recptor II with Src-like protein-tyrosine kinase Fgr in neutrophils." Proc.Natl.Acad.Sci.USA. 90. 6305-6309 (1993)

[Publications] Gojobori,T.: "Genetic variability of major histocompatibility complex in human populations." Human Population Genetics(ed.by P.P.Majumder)Plenum Press,New York. 67-79 (1993)

[Publications] Jinno,A.: "Testis-specific Mak protein kinase is expressed specifically in the meiotic phase in suspermatogenesis and is associated with a 210-kilodalton cellular phosphoprotein." Mol.Cell.Biology. 13. 4146-4156 (1993)

[Publications] Shirasawa,S.: "Alered growth of human colon cancer cell lines disrupted at activated Ki-ras." Science. 260. 85-88 (1993)

[Publications] Kuma,K.: "Motifs of cadherin-and fibronectin Type III-related sequences and evolution of the receptor-Type-protein tyrosine kinases:Sequence similarity between proto-oncogene ret and cadherin family." Mol.Biol.Evol.10. 539-551 (1993)

[Publications] Sugita,Y.: "Determination of a carboxyl-terminal residue and disulfide bonds of MACIF(CD59),a glycosyl-phosphatidyl inositol-anchored" J.Biochem.114. 473-477 (1993)

[Publications] Pinak,M.: "Energy deposition in structural parts of DNA by monoenergetic electrons." J.Radiat.Res.34. 221-234 (1993)

[Publications] Suzuki,T.: "The trans-activator tax of human T-cell leukemia virus type1(HTLV-1)interacts with cAMP-responsive element(CRE)binding and CRE modulator proteins that bind to the 21-base-pair enhancer of HTLV-1." Proc.Natl.Acad.Sci.USA. 90. 610-614 (1993)

黒木登志夫東京大学, 医科学研究所, 教授 (90006073)