1993 Fiscal Year Annual Research Report

細胞の機能分化な関わる遺伝子制御ネットワーク-転写因子間相互作用-

Research Project

Project/Area Number	05273101
Research Institution	Tokyo Institute of Technology
Principal Investigator	半田宏東京工業大学, 生命理工学部, 教授 (80107432)
Co-Investigator(Kenkyū-buntansha)	村松正実埼玉医科大学, 教授 (10035454) 藤沢順一東京大学, 医科学研究所, 助手 (40181341) 藤井義明東北大学, 理学部, 教授 (00098146) 長田重一大阪バイオサイエンス研究所, 部長 (70114428) 石井俊輔理化学研究所, 主任研究員 (00124785) 審良静男大阪大学, 細胞生体工学センター, 助手 (50192919)
Keywords	遺伝子発現 / エンハンサー / 転写制御因子 / RNAポリメラーゼ / 受容体 / シグナル伝達 / コアクチベター / インヒビター
Research Abstract	(1)TFIIAの37kDサブユニットのcDNAをクローン化し、基本転写因子ではなくコアクチベーターとして働くことを示した。F4TF1サブユニットのcDNAをクローン化した(半田)。(2)NF-1L6欠損マウスを作製した。それはリステリア感染に著しく抵抗性が低下し、死亡した。1L-6反応性エレメントに結合するNF-1L6以外の転写因子APRFを精製クローン化した。APRFはISGF3p91と著しい相同性を示した(審良)。(3)c-MybのインヒビターcDNAを得た。Lassin(leucine zipper associated inhibitor)と名付けたインヒビターは68kDのタンパク質で3つのロイシンジッパー構造を持ち、LassinがMybに結合するとMybのDNA結合が阻害されることを明らかにした(石井)。(4)G-CSFは好中球の前駆細胞に作用し、その増殖・分化を誘導する。好中球で特異的に発現される酵素の遺伝子発現を指標として、G-CSF受容体の細胞質領域には、増殖および分化のシグナルを伝達する領域が存在することを示した(長田)。(5)薬物によるP4501A1遺伝子の誘導に働くエンハンサー(XRE)に結合する転写因子Ahレセプター(AhR)とArnt(AhR nuclear translocator)をin vitroで合成した。AhRとArntは3メチルコラントレンなどの誘導剤添加に依存してヘテロ2量体を形成し、XREに結合した。誘導剤によりHSP90からAhRが解離されArntとヘテロ2量体を形成し核へ移行することも示した(藤井)。(6)Taxが転写因子CREB・CREM,NFkBおよびSRFを介して、Taxによって活性化される3種類の異なったエンハンサーに間接的に結合することを明らかにし、架橋因子として転写を活性化する可能性を示した(藤沢)。(7)精製Pollに含まれる53kDタンパク質をクローン化しPAF53と名付けた。これに対する抗体はPollによる転写を特異的に阻害する。UGFとの相互作用も証明され、開始複合体形成に重要な役割を果たす因子と考えられる。rDNAの転写を特異的に抑制する因子を静止期の細胞核内に同定した(村松)。

Research Products
(24 results)

All Other

All Publications (24 results)

[Publications] J-I.Swada et al.: "Transcriptional activation through the tetrameric complex formation of E4TF1 subunitsa." EMBO J.(in press).
[Publications] D.Ma et al.: "Isolation of a cDNA encoding the largest subunit of TFIIA reveals functions imortant for activated transcription." Gens Dev.7. 2246-2257 (1993)
[Publications] H.Watanabe et al.: "cDNA cloning of transcription factor E4TF1 subunits with ETS and NOTCH motifs." Mol.Cell.Biol.13. 1385-1391 (1993)
[Publications] T.Nakajima et al.: "Phosphorylation at threonine 235 by a ras-dependent MAP kinase is essential for transcriptional activation of NF-IL6." Proc.Natl.Acad.Sci.USA. 90. 2207-2211 (1993)
[Publications] Y.Nishio et al.: "A nuclear factor for interlekin6 expression(NF-IL6)and the glucocorticoid receptor synergistically activate transcription of rat α1-acid glycoprotein gene via direct protein-protein interaction." Mol.Cell.Biol.13. 1854-1862 (1993)
[Publications] T.Matsusaka et al.: "NF-IL6 and NF-kB synergistically activate transcription of the inflammatory cytokines,IL-6 and IL-8." Proc.Natl,Acad.Sci.USA. 90. 10193-10197 (1993)
[Publications] T.Kishimoto et al.: "Interactions of cytokines with the receptor family." Cell. (in press).
[Publications] T.Nomura: "Negative autoregulation of c-Myb activity by homodimer formation through the leucine zipper." J.Biol.Chem.168. 21914-21923 (1993)
[Publications] I.Tanikawa et al.: "Recognition of specific DNA sequences by the c-myb proto-oncogene product:role of three repeat units in the DNA binding domain." Proc.Natl.Acad.Sci.USA. 90. 9320-9324 (1993)
[Publications] N.Sakai et al.: "Lassin:a leucine repeat-associated inhibitor of Myb." Genes Dev.(in press).
[Publications] E.Ishizaka-Ikeda et al.: "Signal transduction mediated by the growth hormone receptor and its chimeric molecules with the G-CSF receptor." Proc.Natl.Acad.Sci.USA. 90. 123-127 (1993)
[Publications] J.Ogasawara et al.: "Lethal effect of the anti-Fas antibody in mice." Nature. 364. 806-809 (1993)
[Publications] R.Fukunaga et al.: "Growth and differentiation signals mediated by two different regions in the cytoplasmic domain of G-CSF receptor." Cell. 74. 1079-1087 (1993)
[Publications] T.Suda et al.: "Molecular cloning and expression of the Fas ligand,a novel member of the tumor necrosis factor family." Cell. 75. 1169-1178 (1993)
[Publications] K.Sogawa et al.: "cDNA cloning and transcriptional properties of a novel GC box-binding protein BTEB2." Nucleic Acids Res.21. 1527-1532 (1993)
[Publications] N.Watanabe et al.: "A novel cAMP-dependent regulatory region including a sequence like the cAMP-responsive element upstream of the human CYP21A2 gene." Eur.J.Biochem.214. 521-531 (1993)
[Publications] M.Matsushita et al.: "A factor binding to the xenobiotic responsive element(XRE)of P-4501A1 gene consists of at least two helix-loop-helix proteins,Ah receptor and Arnt." J.Biol.Chem.268. 21002-21006 (1993)
[Publications] J.Fujisawa et al.: "The trans-activator Tax of human T-cell leukemia virus type 1(HTLV-1)interacts with cAMP-responsive element(CRE)binding and CRE modulator proteins that bind to the 21-base-pair enhancer of HTLV-1" Proc.Natl.Acad.Sci.USA. 90. 610-614 (1993)
[Publications] T.Suzuki et al.: "A trans-activator Tax of human T-cell leukemia virus type 1 bimds to NF-kB p50 and serum response factor(SRF)and associates with enhancer DNAs of the NF-kB site and CArG box." Oncogene. 8. 2391-2397 (1993)
[Publications] M.Watanabe et al.: "HTLV-1 encoded Tax in association with NF-kB precursor p105 enhancers nuclear localization of NF-kB p50 and p65 in transfected cells" Oncogene. 8. 2949-2958 (1993)
[Publications] Y.Maeda et al.: "Nuclear targeting of transcription factor UBF directed by both HMG boxes and acidic tail." EMBO J.11. 3695-3704 (1993)
[Publications] Y.Mishima et al.: "Transcription of mouse ribosomal RNA gene with inactivate extracts is activated by NAD in vitro." J.Biochem.113. 36-42 (1993)
[Publications] M.Kermckchiev et al.: "Presence of an inhibitor of RNA polymerase I transcription in extracts from growth arrested mouse cells." Nucleic Acids Res.21. 447-453 (1993)
[Publications] T.Wada et al.: "Methods in Enzymology" DNA affinity chromatography, ((in press))

1993 Fiscal Year Annual Research Report

細胞の機能分化な関わる遺伝子制御ネットワーク-転写因子間相互作用-

Principal Investigator

半田 宏 東京工業大学, 生命理工学部, 教授 (80107432)

Research Products

[Publications] J-I.Swada et al.: "Transcriptional activation through the tetrameric complex formation of E4TF1 subunitsa." EMBO J.(in press).

[Publications] D.Ma et al.: "Isolation of a cDNA encoding the largest subunit of TFIIA reveals functions imortant for activated transcription." Gens Dev.7. 2246-2257 (1993)

[Publications] H.Watanabe et al.: "cDNA cloning of transcription factor E4TF1 subunits with ETS and NOTCH motifs." Mol.Cell.Biol.13. 1385-1391 (1993)

[Publications] T.Nakajima et al.: "Phosphorylation at threonine 235 by a ras-dependent MAP kinase is essential for transcriptional activation of NF-IL6." Proc.Natl.Acad.Sci.USA. 90. 2207-2211 (1993)

[Publications] Y.Nishio et al.: "A nuclear factor for interlekin6 expression(NF-IL6)and the glucocorticoid receptor synergistically activate transcription of rat α1-acid glycoprotein gene via direct protein-protein interaction." Mol.Cell.Biol.13. 1854-1862 (1993)

[Publications] T.Matsusaka et al.: "NF-IL6 and NF-kB synergistically activate transcription of the inflammatory cytokines,IL-6 and IL-8." Proc.Natl,Acad.Sci.USA. 90. 10193-10197 (1993)

[Publications] T.Kishimoto et al.: "Interactions of cytokines with the receptor family." Cell. (in press).

[Publications] T.Nomura: "Negative autoregulation of c-Myb activity by homodimer formation through the leucine zipper." J.Biol.Chem.168. 21914-21923 (1993)

[Publications] I.Tanikawa et al.: "Recognition of specific DNA sequences by the c-myb proto-oncogene product:role of three repeat units in the DNA binding domain." Proc.Natl.Acad.Sci.USA. 90. 9320-9324 (1993)

[Publications] N.Sakai et al.: "Lassin:a leucine repeat-associated inhibitor of Myb." Genes Dev.(in press).

[Publications] E.Ishizaka-Ikeda et al.: "Signal transduction mediated by the growth hormone receptor and its chimeric molecules with the G-CSF receptor." Proc.Natl.Acad.Sci.USA. 90. 123-127 (1993)

[Publications] J.Ogasawara et al.: "Lethal effect of the anti-Fas antibody in mice." Nature. 364. 806-809 (1993)

[Publications] R.Fukunaga et al.: "Growth and differentiation signals mediated by two different regions in the cytoplasmic domain of G-CSF receptor." Cell. 74. 1079-1087 (1993)

[Publications] T.Suda et al.: "Molecular cloning and expression of the Fas ligand,a novel member of the tumor necrosis factor family." Cell. 75. 1169-1178 (1993)

[Publications] K.Sogawa et al.: "cDNA cloning and transcriptional properties of a novel GC box-binding protein BTEB2." Nucleic Acids Res.21. 1527-1532 (1993)

[Publications] N.Watanabe et al.: "A novel cAMP-dependent regulatory region including a sequence like the cAMP-responsive element upstream of the human CYP21A2 gene." Eur.J.Biochem.214. 521-531 (1993)

[Publications] M.Matsushita et al.: "A factor binding to the xenobiotic responsive element(XRE)of P-4501A1 gene consists of at least two helix-loop-helix proteins,Ah receptor and Arnt." J.Biol.Chem.268. 21002-21006 (1993)

[Publications] J.Fujisawa et al.: "The trans-activator Tax of human T-cell leukemia virus type 1(HTLV-1)interacts with cAMP-responsive element(CRE)binding and CRE modulator proteins that bind to the 21-base-pair enhancer of HTLV-1" Proc.Natl.Acad.Sci.USA. 90. 610-614 (1993)

[Publications] T.Suzuki et al.: "A trans-activator Tax of human T-cell leukemia virus type 1 bimds to NF-kB p50 and serum response factor(SRF)and associates with enhancer DNAs of the NF-kB site and CArG box." Oncogene. 8. 2391-2397 (1993)

[Publications] M.Watanabe et al.: "HTLV-1 encoded Tax in association with NF-kB precursor p105 enhancers nuclear localization of NF-kB p50 and p65 in transfected cells" Oncogene. 8. 2949-2958 (1993)

[Publications] Y.Maeda et al.: "Nuclear targeting of transcription factor UBF directed by both HMG boxes and acidic tail." EMBO J.11. 3695-3704 (1993)

[Publications] Y.Mishima et al.: "Transcription of mouse ribosomal RNA gene with inactivate extracts is activated by NAD in vitro." J.Biochem.113. 36-42 (1993)

[Publications] M.Kermckchiev et al.: "Presence of an inhibitor of RNA polymerase I transcription in extracts from growth arrested mouse cells." Nucleic Acids Res.21. 447-453 (1993)

[Publications] T.Wada et al.: "Methods in Enzymology" DNA affinity chromatography, ((in press))

半田宏東京工業大学, 生命理工学部, 教授 (80107432)