1995 Fiscal Year Annual Research Report

植物ホルモン機能の分子機構

Research Project

Project/Area Number	05276102
Research Institution	Hokkaido University
Principal Investigator	山本興太朗北海道大学, 大学院・地球環境科学研究科, 助教授 (80142008)
Co-Investigator(Kenkyū-buntansha)	山口淳二名古屋大学, 生物分子応答研究センター, 助教授 (10183120) 山口五十麿東京大学, 大学院・農学生命科学研究科, 助教授 (00012013) 森仁志名古屋大学, 農学部, 助教授 (20220014) 佐藤隆英千葉大学, 園芸学部, 助教授 (60125929) 高橋陽介東京大学, 大学院・理学系研究科, 助教授 (90183855)
Keywords	植物ホルモン / 遺伝子発現調節 / エチレン生合成 / オーキシン応答シス因子 / ジベレリン結合タンパク / 伸長成長 / 突然変異体(植物ホルモン)
Research Abstract	オーキシンによる伸長成長機構を明らかにするために、下胚軸にオーキシンを与えても偏差成長を起こさない突然変異体を一種類単離した。同変異体は地上部に特異的なオーキシン非感受性突然変異体であったので、オーキシン信号伝達機構は器官別に重複して存在していることが分かった。そこで、下胚軸特異的オーキシン非感受性を指標にして突然変異体を選択したところ、あらたに数種類の変異体を得た。これら変異体の分子遺伝学的研究によって、オーキシンの信号伝達機構を明らかにできると考えられる。オーキシン作用機構研究のもう一つの方向として、オーキシン誘導性遺伝子(ARG)の単離とその機能解析、およびARGプロモーターの誘導機構の研究を行った。本年度は下胚軸伸長成長系で新たに5種類のオーキシン誘導性遺伝子を単離したほか、ARG1と2については、プロモーター領域のクローニングを行った。ARGであるparBのプロモーター解析を行った結果、50から100bpの長さの二つのオーキシン応答性要素がオーキシン誘導に必要であることが分かった。また、もう一つのARGであるarcAの機能を明らかにするために、arcA産物と相互作用するタンパク因子をクローニングした。シベレリン作用に関しては、アミラーゼ遺伝子のジベレリン応答シス配列を解析したほか、伸長反応系でジベレリン誘導性遺伝子を単離した。アズキ上胚軸から可溶性ジベレリン結合タンパクを2,200倍精製しリガンド特異性を調べたところ、各種ジベレリンとの間に高い活性相関があったことから、本標品の生理的意義が高いことが分かった。エチレン生合成調節機構に関しては、個々のACC合成酵素アイソザイムの発現レベルを調べるために特異抗体を作成したほか、同酵素遺伝子プロモーターとレポーター遺伝子の融合遺伝子による形質転換体を作成し、同遺伝子発現調節機構を解析する実験系を作成した。

Research Products

(23 results)

All Other

All Publications (23 results)

[Publications] Watahiki,M.K.: "Inhibitory effects of auxins and their related substances on enzymatic activity of an Arabidopsis glutathione S-transferase isozyme expressed in Escherichia coli." Physiol.Plant.94. 566-574 (1995)
[Publications] Ishida,S.: "The mode of expression and promoter analysis of auxin-regulated arcA gene." Plant Cell Physiol.(印刷中). (1996)
[Publications] Kumagai,F.: "The involvement of protein synthesis elongation factor 1a in the organization of microtubules on the perinuclear region during the cell cycle transition from M phase." Botanica Acta. (印刷中). (1996)
[Publications] Takahashi,Y.: "Identification of auxin-responsive elements of parB and their expression in apices of shoot and root." Proc.Natl.Acad.Sci.USA. 92. 6359-6363 (1995)
[Publications] Takahashi,Y.: "Auxin-regulated genes." Plant Cell Physiol.36. 383-390 (1995)
[Publications] Takahashi,Y.: "Expression of the auxin-regulated parA gene in transgenic tobacco and nuclear localization of its gene products." Planta. 196. 111-117 (1995)
[Publications] Miki,T.: "Nucleotide sequence of a cDNA for 1-aminocyclopropane-1-carboxylate synthase from melon fruits." Plant Physiol.107. 297-298 (1995)
[Publications] Yamamoto,M.: "The synthesis of ethylene in melon fruit during the early stage of ripening." Plant Cell Physiol.36. 591-596 (1995)
[Publications] Nakamura,S.: "Cloning and sequencing of a cDNA for poplar end-1,4-beta-glucanase." Plant Cell Physiol.36. 1229-1235 (1995)
[Publications] Hasegawa,M.: "Immunohistochemistry of gibberellin in rice anthers." Biosci.Biotech.Biochem.59. 1925-1929 (1995)
[Publications] Hasegawa,M.: "Endogenous levels of gibberellins in normal and male sterile anthers of rice(Oryza sativa cv.Nihonmasari)." Biosci.Biotech.Biochem.59. 1716-1720 (1995)
[Publications] Honma,T.: "Endogenous gibberellins in the pollen of Cryptomeria japonica." J.Jpn.For.Soc.77. 358-365 (1995)
[Publications] Yang,Y.-Y.: "Metabolism and translocation of gibberellins in seedlings of Pharbitis nil.(I)Effect of photoperiod on stem elongation and endogenous gibberellins in cotyledons..." Plant Cell Physiol.36. 221-227 (1995)
[Publications] Nakayama,M.: "Qualitative and quantitative analysis of endogenous gibberellins in Raphanus sativus L.during cold treatment and the subsequent growth." Biosci.Biotech.Biochem.59. 1121-1125 (1995)
[Publications] Yang,Y.-Y.: "Metabolism and translocation of gibberellins in the seedlings of Pharbitis nil.(II)Photoperiodic effects on metabolism and translocation of gibberellins..." Plant Cell Physiol.(印刷中). (1996)
[Publications] Oyama,N.: "Gibberellins and antheridiogens in prothallia and sporophytes of Anemiya phyllitidis." Biosci.Biotech.Biochem.(印刷中). (1996)
[Publications] Guglielminetti,L.: "Amylolytic activities in cereal seeds under aerobic and anaerobic conditions." Plant Physiol.109. 1069-1076 (1995)
[Publications] Hatano,S.: "Polymorphism of nuclear DNA from various rice cultivars,as detected by staining of gels after pulsed-field gel electrophoresis." Breeding Sci.45. 205-209 (1995)
[Publications] Itoh,K.: "Developmental and hormonal regulation of rice a-amylase(RAmy1A)-gusA fusion genes in transgenic rice seeds." Plant Physiol.107. 25-31 (1995)
[Publications] Mitsui,T.: "Physicochemical and serological characterization of rice a-amylase isoforms and identification of their corresponding genes." Plant Physiol.(印刷中). (1996)
[Publications] Noda,K.: "Seventh international symposium on pre-harvest sprouting in cereals 1995" 学会出版センター(印刷中), (1996)
[Publications] Lampe,K.: "Rice genetics III" International Rice Research Institute(印刷中), (1996)
[Publications] 種子生理生化学研究会: "種子のバイオサイエンス" 学会出版センター, 454 (1995)

1995 Fiscal Year Annual Research Report

植物ホルモン機能の分子機構

Principal Investigator

山本 興太朗 北海道大学, 大学院・地球環境科学研究科, 助教授 (80142008)

Research Products

[Publications] Watahiki,M.K.: "Inhibitory effects of auxins and their related substances on enzymatic activity of an Arabidopsis glutathione S-transferase isozyme expressed in Escherichia coli." Physiol.Plant.94. 566-574 (1995)

[Publications] Ishida,S.: "The mode of expression and promoter analysis of auxin-regulated arcA gene." Plant Cell Physiol.(印刷中). (1996)

[Publications] Kumagai,F.: "The involvement of protein synthesis elongation factor 1a in the organization of microtubules on the perinuclear region during the cell cycle transition from M phase." Botanica Acta. (印刷中). (1996)

[Publications] Takahashi,Y.: "Identification of auxin-responsive elements of parB and their expression in apices of shoot and root." Proc.Natl.Acad.Sci.USA. 92. 6359-6363 (1995)

[Publications] Takahashi,Y.: "Auxin-regulated genes." Plant Cell Physiol.36. 383-390 (1995)

[Publications] Takahashi,Y.: "Expression of the auxin-regulated parA gene in transgenic tobacco and nuclear localization of its gene products." Planta. 196. 111-117 (1995)

[Publications] Miki,T.: "Nucleotide sequence of a cDNA for 1-aminocyclopropane-1-carboxylate synthase from melon fruits." Plant Physiol.107. 297-298 (1995)

[Publications] Yamamoto,M.: "The synthesis of ethylene in melon fruit during the early stage of ripening." Plant Cell Physiol.36. 591-596 (1995)

[Publications] Nakamura,S.: "Cloning and sequencing of a cDNA for poplar end-1,4-beta-glucanase." Plant Cell Physiol.36. 1229-1235 (1995)

[Publications] Hasegawa,M.: "Immunohistochemistry of gibberellin in rice anthers." Biosci.Biotech.Biochem.59. 1925-1929 (1995)

[Publications] Hasegawa,M.: "Endogenous levels of gibberellins in normal and male sterile anthers of rice(Oryza sativa cv.Nihonmasari)." Biosci.Biotech.Biochem.59. 1716-1720 (1995)

[Publications] Honma,T.: "Endogenous gibberellins in the pollen of Cryptomeria japonica." J.Jpn.For.Soc.77. 358-365 (1995)

[Publications] Yang,Y.-Y.: "Metabolism and translocation of gibberellins in seedlings of Pharbitis nil.(I)Effect of photoperiod on stem elongation and endogenous gibberellins in cotyledons..." Plant Cell Physiol.36. 221-227 (1995)

[Publications] Nakayama,M.: "Qualitative and quantitative analysis of endogenous gibberellins in Raphanus sativus L.during cold treatment and the subsequent growth." Biosci.Biotech.Biochem.59. 1121-1125 (1995)

[Publications] Yang,Y.-Y.: "Metabolism and translocation of gibberellins in the seedlings of Pharbitis nil.(II)Photoperiodic effects on metabolism and translocation of gibberellins..." Plant Cell Physiol.(印刷中). (1996)

[Publications] Oyama,N.: "Gibberellins and antheridiogens in prothallia and sporophytes of Anemiya phyllitidis." Biosci.Biotech.Biochem.(印刷中). (1996)

[Publications] Guglielminetti,L.: "Amylolytic activities in cereal seeds under aerobic and anaerobic conditions." Plant Physiol.109. 1069-1076 (1995)

[Publications] Hatano,S.: "Polymorphism of nuclear DNA from various rice cultivars,as detected by staining of gels after pulsed-field gel electrophoresis." Breeding Sci.45. 205-209 (1995)

[Publications] Itoh,K.: "Developmental and hormonal regulation of rice a-amylase(RAmy1A)-gusA fusion genes in transgenic rice seeds." Plant Physiol.107. 25-31 (1995)

[Publications] Mitsui,T.: "Physicochemical and serological characterization of rice a-amylase isoforms and identification of their corresponding genes." Plant Physiol.(印刷中). (1996)

[Publications] Noda,K.: "Seventh international symposium on pre-harvest sprouting in cereals 1995" 学会出版センター(印刷中), (1996)

[Publications] Lampe,K.: "Rice genetics III" International Rice Research Institute(印刷中), (1996)

[Publications] 種子生理生化学研究会: "種子のバイオサイエンス" 学会出版センター, 454 (1995)

山本興太朗北海道大学, 大学院・地球環境科学研究科, 助教授 (80142008)