1996 Fiscal Year Annual Research Report

植物細胞形態構築の制御機構

Research Project

Project/Area Number	05276103
Research Institution	Osaka University
Principal Investigator	柴岡弘郎大阪大学, 大学院・理学研究科, 教授 (60087054)
Co-Investigator(Kenkyū-buntansha)	福田裕穂東京大学, 理学部, 教授 (10165293) 保尊隆享大阪市立大学, 理学部, 助教授 (70135771) 西谷和彦鹿児島大学, 教養部, 助教授 (60164555) 園部誠司姫路工業大学, 理学部, 助教授 (30197024) 馳澤盛一郎東京大学, 理学研究科, 助教授 (40172902)
Keywords	植物ホルモン / 細胞形態形成 / 細胞伸長 / 細胞壁 / 微小管 / 細胞分化 / 遺伝子発現
Research Abstract	植物ホルモンは細胞伸長および細胞伸長の方向を制御することにより細胞一個一個の形を制御しているが、それだけでなく、細胞分化にも大きな影響を与えている。本研究は植物ホルモンの細胞伸長制御機構、伸長方向制御機構、細胞分化制御機構の解明に重点を置き行われたものである。植物ホルモンによる細胞伸長方向の制御には細胞質表層微小管が関与している。柴岡は、アズキ上胚軸の表皮細胞を用いて表層微小管の方向制御に関する研究を行い、オーキシンによる微小管の縦方向から横方向への方向変更が、蛋白質脱リン酸化酵素の阻害剤であるオカダ酸により阻害されることを見いだし、微小管の縦方向から横方向への方向変更に蛋白質の脱リン酸化が必要であることを明らかにした。馳沢は、タバコBY-2細胞を用いて微小管重合中心活性を持つ蛋白質の探索を行い、候補として、100kDaおよび60kDaの蛋白質を得た。園部は、タバコ培養細胞の脱液胞プロトプラストより得た蛋白質分画に、微小管を細胞膜に架橋させる活性があることを明らかにした。西谷は、シロイヌナズナのエンド型キシログルカン転移酵素遺伝子が、どの器官においても成熟した細胞で高い発現を示すことを明らかにし、この酵素が、二次細胞壁の構築に関与している可能性が高いことを示した。林は、ポプラ培養細胞において、オーキシンがエンド-1,4-β-グルカナーゼ活性を増加させること、また、細胞壁キシログルカンの可溶化を生じさせることを明らかにした。保尊は、アズキ上胚軸中に存在する新しいキシログルカン分解酵素を発見したが、この酵素はエンド型キシログルカン転移酵素であるが、より低分子のアクセプターを要求するため結果的に加水分解作用を持つことを明らかにした。福田は、ヒャクニチソウ単離葉肉細胞の管状要素細胞への分化の後期過程に起きる遺伝子発現に、細胞中で合成されるブラシノステロイドが必要であることを明らかにした。また、管状要素分化に伴って発現する遺伝子のアンチセンスDNAを導入した形質転換根を作成し、遺伝子機能の解析を試みた。

Research Products

(21 results)

All Other

All Publications (21 results)

[Publications] SHIBAOKA,H.: "The use of madel systems prepared from tobacco BY-2 cells for studies of the plant cytoskeleton" J.Microscopy. 181. 145-152 (1996)
[Publications] MAYUMI,K.: "The cyclic reorientation of cortical microtubules on walls with a crossed polylamellate structure" Protoplasma. 195. 112-122 (1996)
[Publications] ASADA,I.: "TKRP125,a kinesin-related protein involved in the centrosome-independent organization of the cytokinetic apparatus in tebacco BY-2 cells" J.Cell Sci.110. 179-189 (1997)
[Publications] HIGASHIYAMA,T.: "Identification of novel 70 KDa protein in cultured tobacco cells that is immunologically related to MAP4" Cytologia. 61. 229-233 (1996)
[Publications] MITSUI,H.: "Cell cycle-dependent accumulation of a kinesin-like protein,Kat B/C,in synchron:Zed tobacco BY-2 celts" Plant Mol.Biol.30. 177-181 (1996)
[Publications] MIYAKE,T.: "Role of cytoskeletal components in the migration of nucki during the cell cycle trausition from G1 phase to S phase of tobacco BY-2 cells" J.Plant Physiol.(in press). (1997)
[Publications] SONOBE,S.: "Studies on the plant cytoskeleton using miniprotoplasts of tobacco BY-2 cells" J.Plant Res.109. 437-448 (1996)
[Publications] SONOBE,S.: "Cell model systems in plant cytoskeleton studies" Int.Rev.Cytol.(in press). (1997)
[Publications] NISHITANI,K.: "The role of endoxylogiucan transferase in the organization of plant cell walls" Int.Rev.Cytol.173(in press). 157-206 (1997)
[Publications] NISHITANI,K.: "Implication of xyloglucan related protein(XRP)family in regulation of plant growth and development" Trends Glycosci,Glycotech.(in press). (1997)
[Publications] TAKEDA,T.: "Xyloglucan endotrans-glycosylation in suspensim-cedtured poplar cells" Biosci.Biotech.Biochem.60. 1950-1955 (1996)
[Publications] MATSUMOTO,T.: "Purification of xyloglucanase from auxin-treated pea stems" Wood Res.83. 21-22 (1996)
[Publications] TAKEDA,T.: "Gene expression of endo-1,4-β-glucanase in suspension cultured poplar cells" Biosci.Biotech.Biochem.1997 ((in press))
[Publications] MATSUMOTO,T.: "A xyloglucan-specific endo-1,4-β-glucanase isolated from auxin-treated pea stems" Plant Physiol.1997 ((in press))
[Publications] HOSON,T.: "Ettects of hypergravity on growth and cell wall properties of cress hypocotyls" J.Exp.Bot.47. 513-518 (1996)
[Publications] TABUCHI,A.: "Purification of xylogluean hydrolase/endutransferase from cell walls of azulki bean epicotyls" Plant Cell Physiol.38(in press). (1997)
[Publications] FUKUDA,H.: "Xylogenesis:Initiation,progression and cen death" Annu.Rev.Plant Physiol.Plant Mol.Biol.47. 299-325 (1996)
[Publications] SATO,K.: "Purification and characterization of a mitochondrial DNA polymerase from cultured tobacco cells" Plant Cell Physiol.37. 989-995 (1996)
[Publications] YOSHIMURA,T.: "Differential expression of three genes for different β-tubulin isofornis during the initial culture of Zinria mesophyll cells that divide and differaentiate into tracueary elements" Plant Cell Physiol.37. 1167-1176 (1996)
[Publications] DEMURA,T.: "In situ hybridization to cellular RNA using ^<35>S-labeled cRNA probes" Plant Tissue Culture Letters. 13. 343-349 (1996)
[Publications] SATO,Y.: "Changes in the activity and mRNA of ciunamyl alcouol dehydrogenase during tracheary element differentiation in Zinnia elegans" Plant Physiol.(in press). (1997)

1996 Fiscal Year Annual Research Report

植物細胞形態構築の制御機構

Principal Investigator

柴岡 弘郎 大阪大学, 大学院・理学研究科, 教授 (60087054)

Research Products

[Publications] SHIBAOKA,H.: "The use of madel systems prepared from tobacco BY-2 cells for studies of the plant cytoskeleton" J.Microscopy. 181. 145-152 (1996)

[Publications] MAYUMI,K.: "The cyclic reorientation of cortical microtubules on walls with a crossed polylamellate structure" Protoplasma. 195. 112-122 (1996)

[Publications] ASADA,I.: "TKRP125,a kinesin-related protein involved in the centrosome-independent organization of the cytokinetic apparatus in tebacco BY-2 cells" J.Cell Sci.110. 179-189 (1997)

[Publications] HIGASHIYAMA,T.: "Identification of novel 70 KDa protein in cultured tobacco cells that is immunologically related to MAP4" Cytologia. 61. 229-233 (1996)

[Publications] MITSUI,H.: "Cell cycle-dependent accumulation of a kinesin-like protein,Kat B/C,in synchron:Zed tobacco BY-2 celts" Plant Mol.Biol.30. 177-181 (1996)

[Publications] MIYAKE,T.: "Role of cytoskeletal components in the migration of nucki during the cell cycle trausition from G1 phase to S phase of tobacco BY-2 cells" J.Plant Physiol.(in press). (1997)

[Publications] SONOBE,S.: "Studies on the plant cytoskeleton using miniprotoplasts of tobacco BY-2 cells" J.Plant Res.109. 437-448 (1996)

[Publications] SONOBE,S.: "Cell model systems in plant cytoskeleton studies" Int.Rev.Cytol.(in press). (1997)

[Publications] NISHITANI,K.: "The role of endoxylogiucan transferase in the organization of plant cell walls" Int.Rev.Cytol.173(in press). 157-206 (1997)

[Publications] NISHITANI,K.: "Implication of xyloglucan related protein(XRP)family in regulation of plant growth and development" Trends Glycosci,Glycotech.(in press). (1997)

[Publications] TAKEDA,T.: "Xyloglucan endotrans-glycosylation in suspensim-cedtured poplar cells" Biosci.Biotech.Biochem.60. 1950-1955 (1996)

[Publications] MATSUMOTO,T.: "Purification of xyloglucanase from auxin-treated pea stems" Wood Res.83. 21-22 (1996)

[Publications] TAKEDA,T.: "Gene expression of endo-1,4-β-glucanase in suspension cultured poplar cells" Biosci.Biotech.Biochem.1997 ((in press))

[Publications] MATSUMOTO,T.: "A xyloglucan-specific endo-1,4-β-glucanase isolated from auxin-treated pea stems" Plant Physiol.1997 ((in press))

[Publications] HOSON,T.: "Ettects of hypergravity on growth and cell wall properties of cress hypocotyls" J.Exp.Bot.47. 513-518 (1996)

[Publications] TABUCHI,A.: "Purification of xylogluean hydrolase/endutransferase from cell walls of azulki bean epicotyls" Plant Cell Physiol.38(in press). (1997)

[Publications] FUKUDA,H.: "Xylogenesis:Initiation,progression and cen death" Annu.Rev.Plant Physiol.Plant Mol.Biol.47. 299-325 (1996)

[Publications] SATO,K.: "Purification and characterization of a mitochondrial DNA polymerase from cultured tobacco cells" Plant Cell Physiol.37. 989-995 (1996)

[Publications] YOSHIMURA,T.: "Differential expression of three genes for different β-tubulin isofornis during the initial culture of Zinria mesophyll cells that divide and differaentiate into tracueary elements" Plant Cell Physiol.37. 1167-1176 (1996)

[Publications] DEMURA,T.: "In situ hybridization to cellular RNA using ^<35>S-labeled cRNA probes" Plant Tissue Culture Letters. 13. 343-349 (1996)

[Publications] SATO,Y.: "Changes in the activity and mRNA of ciunamyl alcouol dehydrogenase during tracheary element differentiation in Zinnia elegans" Plant Physiol.(in press). (1997)

柴岡弘郎大阪大学, 大学院・理学研究科, 教授 (60087054)