1994 Fiscal Year Annual Research Report

生物機能の時空間制御を目指した有機無機複合体の開発

Research Project

Project/Area Number	05303008
Research Institution	NARA WOMEN'S UNIVERSITY
Principal Investigator	矢野重信奈良女子大学, 理学部, 教授 (60011186)
Co-Investigator(Kenkyū-buntansha)	藤井有起茨城大学, 理学部, 教授 (50007564) 長野哲雄東京大学, 薬学部, 助教授 (20111552) 桜井弘京都薬科大学, 薬学部, 教授 (30065916) 大塚雅巳京都大学, 化学研究所, 助教授 (40126008) 大倉一郎東京工業大学, 生命理工学部, 教授 (90089821)
Keywords	抗菌活性 / 分子認識 / 配糖錯体 / 活性酸素 / DNA / 血糖値の制御 / インターカレーション / シッフ塩基
Research Abstract	本年度は昨年度からの研究を引き続いて行うとともに各班員により次の様な研究を展開した。矢野、加藤はD-グルコサミンN(II)配糖錯体が病原性酵母Candida albicansの成育を有効に阻止することを明らかにした。青山は水素結合や金属配位結合を利用することにより結晶構造を規制した。大倉はスルホ基数の異なる亜鉛フタロシアニンを合成し、光化学療法における有用性を検討した。大塚はピリジンとヒスチジンからなる人口配位子を合成し、DNAのκB配列に特異的に結合する蛋白質NF-κBおよびHIV-EP1の活性化やDNAとの結合の阻害を明らかにした。小谷は水素液中で金属錯体が関与した弱い相互作用の強さとその構造依存性を調べた。後藤は白金(II)錯体における配位子間相互作用を調べた。桜井はセレン及びバナジル錯体による血糖値制御を議論した。谷口は金属タンパク質の速い電子移動の制御を生体機能の解明に関連して行った。千喜良はDNAファイバーESRにより、過酸化水素存在下でのDNA切断反応は解離した銅(II)イオンによることを推定した。中井はEu(III)錯体を触媒とする不斉アルドール反応では、「分子認識に基づく触媒的立体化学制御」が効果的に達成されることを明らかにした。長野はsuperoxide dismutase活性を有する鉄錯体(Fe・TPENとその類縁体)を合成し、活性と構造の相関を検討し反応機構の解析を行った。西田は各種鉄キレートを動物に注射し、その効果を細胞死と発ガン作用との関連性から検討した。廣瀬はPt(1R,2R-diaminocyclohexane)-DNA化合物に対するモノクローナル抗体を調製した。藤井は5位にトリアルキルアンモニウムメチル基を導入したカチオン性salen型シッフ塩基を配位子とする錯体を合成し、DNAとの結合定数を求めた。一方、DNA切断の観点から、亜鉛(II)-トリアミン錯体の燐酸エステル加水分解能について検討した。山村はCys-X-Y-Cys型のペプチドの水銀と亜鉛錯体の構造と蛋白質中の配位と配座の相関について考察した。

Research Products

(16 results)

All Other

All Publications (16 results)

[Publications] Y.Sugihara: "The Structure of Diethyl(3-pyridyl)borane" J.Chem.Soc.,Chem.Commun.1925-1926 (1994)
[Publications] K.Kobayashi: "The Crystal Structure of a Bisresorcinol Derivative of Anthracene Hydrogen-Bonded Network Formation in Orga ic Crystals as Effected by Perpendicular and Divergent Hydroxyl Groups" Tetrahedron Lett.34. 7929-7932 (1993)
[Publications] T.Takeno: "Characterization of Sulfonated Zinc Phthalocyaninesas Photosensitizers for Photodynamic Therapy" Lasers in the Life Sciences. 6. 173-179 (1994)
[Publications] T.Sugiyama: "Transition-Metal Binding Site of Bleomycin.Significance of the β-Aminoalaninamide Appendagein Regulating Oxygen Activation" Heterocycles. 37. 275-282 (1994)
[Publications] Y.Funahashi: "Spectroscopic Evidence for the Redox Reaction between Copper(II)and a Pterin Cofactor Model・・・・" Chem.Lett.385-388 (1994)
[Publications] M.Goto: "Dependence of Dehydrogenation of Amines towards Coordination Geometry:Oxidation Products of Tricyano〔di(2-pyridylmethyl)amine〕ferrate(II)from mer and fac Isomers" J.Chem.Soc.,Chem.Commun.2015-2016 (1994)
[Publications] H.Sakurai: "Unusual generation of hydroxyl radicals in hepatic copper-metallothionein of LEC(Long-Evans Cinnamon)rats in the presence of hydrogen peroxide" Biochem.Biophys.Res.Commun.199. 313-318 (1994)
[Publications] I.Taniguchi: "Potential-Dependent Rectified Electron Transfer at a Meldola's Blue Incorporated Decanethiol Modified Electrodeas a Model of Biological Membrane" Microchem.J.49. 340-354 (1994)
[Publications] M.Chikira: "Orientations of Copper(II)Complexes of 1,10-Phenanthroline and Its Derivatives on DNA-fibers" Mag.Res.Medicine. 6. 214-215 (1995)
[Publications] T.Nakai: "[2.3]Wittig Sigmatropic Rearrangement" Organic Reactions. 46. 105-109 (1994)
[Publications] H.Hayakawa: "Long-Tem Administration of L-Arginine Improves Nitric Oxide Release from Kidney in DOCA Salt Hypertensive Rats" Hypertension. 23. 752-756 (1994)
[Publications] Y.Nishida: "Iron Chelates in Biological Systems.Its Relevance to Induction of Pathogenesis of Tissue Damage and Carcinogenesis" Chem.Lett.641-644 (1994)
[Publications] K.Sato: "Stereospecific Binding of Chemically Modified Salen-type Schiff Base Complexes of Copper(II)with DNA" J.Chem.Soc.,Chem.Commun.625-626 (1994)
[Publications] J.Hirose: "Characterization of Ascorbate Oxidase from Acremoniumsp. HI-25" J.Biochem.115. 811-813 (1994)
[Publications] T.Yamamura: "Confomation Control of Model Peptides by Metal Ions I.Cys-X-Y-Cys and Linear Coordination" Phys.Chem.
[Publications] T.Tanase: "Chiral Inversion around the Seven-Coordinated Cobalt Cneter Induced by an Interaction between Sugars and Tetrahedral Oxoanions." Inorg.Dhem.33. 5-6 (1994)

1994 Fiscal Year Annual Research Report

生物機能の時空間制御を目指した有機無機複合体の開発

Principal Investigator

矢野 重信 奈良女子大学, 理学部, 教授 (60011186)

Research Products

[Publications] Y.Sugihara: "The Structure of Diethyl(3-pyridyl)borane" J.Chem.Soc.,Chem.Commun.1925-1926 (1994)

[Publications] K.Kobayashi: "The Crystal Structure of a Bisresorcinol Derivative of Anthracene Hydrogen-Bonded Network Formation in Orga ic Crystals as Effected by Perpendicular and Divergent Hydroxyl Groups" Tetrahedron Lett.34. 7929-7932 (1993)

[Publications] T.Takeno: "Characterization of Sulfonated Zinc Phthalocyaninesas Photosensitizers for Photodynamic Therapy" Lasers in the Life Sciences. 6. 173-179 (1994)

[Publications] T.Sugiyama: "Transition-Metal Binding Site of Bleomycin.Significance of the β-Aminoalaninamide Appendagein Regulating Oxygen Activation" Heterocycles. 37. 275-282 (1994)

[Publications] Y.Funahashi: "Spectroscopic Evidence for the Redox Reaction between Copper(II)and a Pterin Cofactor Model・・・・" Chem.Lett.385-388 (1994)

[Publications] M.Goto: "Dependence of Dehydrogenation of Amines towards Coordination Geometry:Oxidation Products of Tricyano〔di(2-pyridylmethyl)amine〕ferrate(II)from mer and fac Isomers" J.Chem.Soc.,Chem.Commun.2015-2016 (1994)

[Publications] H.Sakurai: "Unusual generation of hydroxyl radicals in hepatic copper-metallothionein of LEC(Long-Evans Cinnamon)rats in the presence of hydrogen peroxide" Biochem.Biophys.Res.Commun.199. 313-318 (1994)

[Publications] I.Taniguchi: "Potential-Dependent Rectified Electron Transfer at a Meldola's Blue Incorporated Decanethiol Modified Electrodeas a Model of Biological Membrane" Microchem.J.49. 340-354 (1994)

[Publications] M.Chikira: "Orientations of Copper(II)Complexes of 1,10-Phenanthroline and Its Derivatives on DNA-fibers" Mag.Res.Medicine. 6. 214-215 (1995)

[Publications] T.Nakai: "[2.3]Wittig Sigmatropic Rearrangement" Organic Reactions. 46. 105-109 (1994)

[Publications] H.Hayakawa: "Long-Tem Administration of L-Arginine Improves Nitric Oxide Release from Kidney in DOCA Salt Hypertensive Rats" Hypertension. 23. 752-756 (1994)

[Publications] Y.Nishida: "Iron Chelates in Biological Systems.Its Relevance to Induction of Pathogenesis of Tissue Damage and Carcinogenesis" Chem.Lett.641-644 (1994)

[Publications] K.Sato: "Stereospecific Binding of Chemically Modified Salen-type Schiff Base Complexes of Copper(II)with DNA" J.Chem.Soc.,Chem.Commun.625-626 (1994)

[Publications] J.Hirose: "Characterization of Ascorbate Oxidase from Acremoniumsp. HI-25" J.Biochem.115. 811-813 (1994)

[Publications] T.Yamamura: "Confomation Control of Model Peptides by Metal Ions I.Cys-X-Y-Cys and Linear Coordination" Phys.Chem.

[Publications] T.Tanase: "Chiral Inversion around the Seven-Coordinated Cobalt Cneter Induced by an Interaction between Sugars and Tetrahedral Oxoanions." Inorg.Dhem.33. 5-6 (1994)

矢野重信奈良女子大学, 理学部, 教授 (60011186)