1994 Fiscal Year Final Research Report Summary
Molecular Biology of the Kidney-Molecular Analysis of Structure and Function Relationship
| Project/Area Number |
05304037
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| Research Category |
Grant-in-Aid for Co-operative Research (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
Kidney internal medicine
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
MARUMO Fumiaki Tokyo Medical & Dental Univ.Internal Medicine.Professor, 医学部, 教授 (00050443)
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| Co-Investigator(Kenkyū-buntansha) |
SAKAI Osamu Tokyo Jikei Medical University.Internal Medicine.Professor, 医学部, 教授 (40056560)
KOIDE Hikaru Jyuntendo University.Internal Medicine.Professor, 医学部, 教授 (80052968)
KUROKAWA Kiyoshi Tokyo University.Internal Medicine.Professor, 医学部, 教授 (30167390)
OKUDA Seiya Kyushuu University.Internal Medicine.Assistant Professor, 医学部, 講師 (80158823)
ARAKAWA Masaaki Niigata University.Internal Medicine.Professor, 医学部, 教授 (80069012)
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| Project Period (FY) |
1993 – 1994
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| Keywords | Molecular biology / Nephrology / Cloning / Glomerulus / Nephron / Transporter / Basement Membrane / Hormone |
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| Research Abstract |
We have investigated three major aspects of the kidney.
1) Glomerular matrix and glomerular function. We found a mutations of alpha 5 chain of typelV collagen gene in patients with Alport syndrome. This result confirm the significance of basement membrane collagen in glomerular function. We also showed that glycosilated substances stimulates the transcription of extracellular matrix genes. This stimulation is important for the sclorotic changes of glomerulus frequently observed in diabetes meditus.
2) Regulation of bioactive substances and their receptors. We showed the presence of TGFb mRNA in glomerulus, all nephron segments, small renal arteiries. On the other hand, mRNA of LTBP,which is important for activation of TGFb, is present only in glomerulus and small renal arteiries. We also developed a new acurate method for quantification of RTPCR which is critically needed for small piece of tissues such as dissected nephron segments. Usin this method we showed the nephron localization of mRNA of PGE2 receptor.
3) Cloning and characterization of kidney trnasport proteins. We cloned a kidney collecting duct water channel named AQP3, which is localized at the basolateral membrane. We showed many regulational mechanisms of AQP2 at trnascriptional, protein, and cellular levels. We anso cloned a rat dipeptide transporter in the kidney, and found that this transporter works as a rout for cepharosporin antibiotics.
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