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1995 Fiscal Year Final Research Report Summary

Design of Membrane Surfaces for Molecular Recognition and Signal Transduction, and Their Application for Chemical Sensing

Research Project

Project/Area Number 05403016
Research Category

Grant-in-Aid for General Scientific Research (A)

Allocation TypeSingle-year Grants
Research Field 分離・精製・検出法
Research InstitutionThe University of Tokyo

Principal Investigator

UMEZAWA Yoshio  The University of Tokyo Graduate School of Science Professor, 大学院・理学系研究科, 教授 (80011724)

Co-Investigator(Kenkyū-buntansha) BUHLMANN Philippe  The University of Tokyo Graduate School of Science Assistant, 大学院・理学系研究科, 助手 (20262149)
TOHDA Koji  The University of Tkoyo Graduate School of Science Assistant, 大学院・理学系研究科, 助手 (60212065)
SUGAWARA Masao  The University of Tokyo Graduate School of Science Associate Professor, 大学院・理学系研究科, 助教授 (50002176)
Project Period (FY) 1993 – 1995
KeywordsCharge separation / Ion channel / Multitopic molecular recognition / Active transport / Condensed monolayer / Second harmonic generation (SHG) / Bilayer lipid membrane / Infrared attenuated total reflection (ATR-IR)
Research Abstract

1. Chemical Sensing Based on Charge Separation
1) New liquid membrane potentiometric sensors for the discrimination of organic analytes based on host-guest recognition of charged groups, hydrogen bonding groups, or steric shapes of nonpolar moieties were developed. A membrane sensor using a cytosine-pendant triamine host showed potentiometric discrimination between guanosine and adenosine nucleotides by ditopic recognition, i.e., complementary base pairing and phosphate-ammonium charge-charge binding interactions at the membrane surface. A calix [6] arene derivative having a well-defined inclusion cavity for organic guests was synthesized and used as a sensory element for the potentiometric discrimination of the steric shapes of nonpolar moieties of dopamine.
2) Permselective ion transport for metal cations at the interface (^-mum depth) of ionophore-based liquid membranes was successfully observed by infrared attenuated total reflection spectrometry (ATR-IR). Furthermore, optical second … More harmonic generation (SHG) was shown to be the first optical technique available for the selective observation of charge separation processes at the surface of liquid membranes. By observing the SHG signals from ionophore-based liquid membranes, the existence of SHG-active, oriented cation complexes at the membrane surface was shown.
2. Chemical Sensing Based on Changes in Membrane Permeability
The control of membrane permeability through intermolecular channels by host-guest complexation was exploited by horizontal tough cyclic voltammetry with condensed monolayrs of long alkyl derivatives of beta-cyclodextrin formed at the air-water interface. Comparing the permeabilities of three kinds of electroactive markers, rigid evidence was obtained that this cyclodextrin derivative functions as an intramolecular channel. The selectivity of permeability inhibition by several organic guest was found to reflect the ability of the quests to block the channel entrance by host-guest complexation.
3. Chemical Sensing Based on Active Transport
Based on highly selective recognition and following efficient transport of D-glucose by Na^+/D-glucose contransporter, a bilayer lipid membrane sensing system for D-glucose was constructed. This active transport sensor enables detection of D-glucose concentrations as low as 10^<-9>M. Less

  • Research Products

    (45 results)

All Other

All Publications (45 results)

  • [Publications] K.Yagi: "Channel Mimetic Sensing Membranes for Alkali Metal Cations Based on Oriented Monolayers of Calixarene Esters." J.Electroanal.Chem.and Interfacial Electrochem.(in press).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M.Sugawara: "Concentration of Dopamine by Proton-Driven Uphill Transport Using Lasalocid A as the Carrer." Anal.Sci.(in press).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] S.Nishizawa: "Anion Recognition by Urea and Thiourea Groups : Remarkably Simple Neutral Receptors for Dihydrogenphosphate." Tetrahedron Letters. 36. 6483-6486 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] P.Buhlmann: "Studies on the Phase Boundaries and the Significance of Ionic Sites of Liquid Membrane Ion-Selective Electrodes." Electroanalysis. 7. 811-816 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] P.Buhlmann: "EMF Response of Neutral Carrier Based Ion-Selective Field Effect Transistors with Membranes Free of Ionic Sites" Electrochim.Acta. 40. 3021-3027 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M.Namba: "Molecular Resolution Images of a Calix [6] arene from Atomic Force Microscopy." Langmuir. 11. 635-638 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y.Umezawa: "Selectivity Coefficients for Ion-Selective Electrodes : Recommended Methods for Reporting K<pot【chemical formula】,B> Values." Pure & Appl.Chem.67. 507-518 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.Tohda: "Cation Permselectivity at the Phase Boundary of Ionophore-Incorporated Solvent Polymeric Membranes as Studied by Optical Second Harmonic Generation." Anal.Chem.67. 570-577 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.Odashima: "Channel Mimetic Sensing Membranes Based on Host-Guest Molecular Recognition by Synthetic Receptors." Interfacial Design and Chemical Sensing. 123-134 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y.Tani: "Non-Stoichiometric Dissolution of Lanthanum Fluoride (LaF_3) and Its Relevance to a Process of Ion-Selective Charge Separation at the Solid/ Solution Interface." J.Electroanal.Chem.and Interfacial Electrochem.378. 205-213 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M.Sugawara: "Factors Affecting Background Permeabilities of Ordered Mono-,Bi-,and Multilayers as Channel Mimetic Sensing Membranes." Anal.Sci.10. 343-347 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.Odashima: "Chemical Sensing Based on Membrane Potential Change Induced by Host-Guest Complexation at Membrane Surface." Supramol.Chem.4. 101-113 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.Odashima: "Potentiometric Discrimination of Organic Amines by a Liquid Membrane Electrode Based on a Long Alkyl Chain Derivative of β-Cyclodextrin." Mikrochim.Acta. 113. 223-238 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y.Umezawa: "Development of Biological and Synthetic Receptor-Embedded Lipid Membrare Assemblies with Molecular Recognition and Signal Transduction/Amplification Functions." New Functionality Materials B. 111-116 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] R.Naganawa: "Potentiometric Discrimination Between Planar and Octahedral Anionic Metal Cyano Complexes by Liquid Membrane Electrodes Based on Lipophilic Macrocyclic Dioxopolyamines." Electroanalysis. 5. 731-738 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.Umezawa: "Cation Permselectivity in the Phase Boundary of Ionophore-Incorporated Solvent Polymeric Membranes as Studied by Fourier Transform Infrared Attenuated Total Reflection Spectrometry." Anal.Chim.Acta. 282. 247-257 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.Odashima: "Voltammetric Study on a Condensed Monolayer of a Long Alkyl Cyclodextrin Derivative as a Channel Mimetic Sensing Membrane." Anal.Chem.65. 927-936 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.Odashima: "Potentiometric Discrimination of Organic Amines by a Liquid Membrane Electrode Based on a Lipophilic Hexaester of Calix [6] arene." Anal.Chem.65. 1074-1083 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] S.Yoshiyagawa: "Optical Ion Sensor Based on Second-Harmonic Generation at the Surface of an Ionophore-Incorporated Poly (vinyl chloride) Liquid Membrane." Anal.Sci.9. 715-718 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] N.Sugao: "Na^+/D-Glucose Cotransporter Based Bilayer Lipid Membrane Sensor for D-Glucose." Anal.Chem.65. 363-369 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.Tohda: "Liquid Membrane Electrodes for Nucleotides Based on Sapphyrin,Cytosine-Pendant Triamine and Neutral Cytosine Derivative as Sensory Elements." Sensors and Actuators B. 13-14. 669-672 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.Tohda: "Liquid Membrane Electrode for Guanosine Nucleotides Using a Cytosine-Pendant Triamine Host at the Sensory Element." Anal.Chem.64. 960-964 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.Yagi: "Channel Mimetic Sensing Membranes for Alkali Metal Cations Based on Oriented Monolayrs of Calixarene Esters." J.Electroanal.Chem.and Interfacial Electrochem.(in press).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M.Sugawara: "Concentration of Dopamine by Proton-Driven Uphill Transport Using Lasalocid A as the Carrier." Anal.Sci.(in press).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S.Nishizawa: "Anion Recognition by Urea and Thiourea Groups : Remarkably Simple Neutral Receptors for Dihydrogenphosphate." Tetrahedron Letters. 36, No.36. 6483-6486 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] P.Buhlmann: "Studies on the Phase Boundaries and the Significance of Ionic Sites of Liquid Membrane Ion-Selective Electrodes." Electroanalysis. 7. 811-816 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] P.Buhlmann: "EMF Response of Neutral Carrier Based Ion-Selective Field Effect Transistors with Membranes Free of Ionic Sites." Electrochim.Acta. 40, No.18. 3021-3027 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M.Namba: "Molecular Resolution Images of a Calix [6] arene from Atomic Force Microscopy." Langmuir. 11. 635-638 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y.Umezawa: "Selectivity Coefficients for Ion-Selective Electrodes : Recommended Methods for Reporting K^<pot>_<A.B> Values." Pure & Appl.Chem.67. 507-518 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.Tohda: "Cation Permselectivity at the Phase Boundary of Ionophore-Incorporated Solvent Polymeric Membranes as Studied by Optical Second Harmonic Generation." Anal.Chem.67. 570-577 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.Odashima: "Channel Mimetic Sensing Membranes Based on Host-Guest Molecular Recognition by Synthetic Receptors." Interfacial Design and Chemical Sensing, T.E.Mallouk and D.J.Harrison (eds.). Chap.11. 123-134 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y.Tani: "Non-Stoichiometric Dissolution of Lanthanum Fluoride (LaF_3) and Its Relevance to a Process of Ion-Selective Charge Separation at the Solid/Solution Interface." J.Electroanal.Chem.and Interfacial Electrochem.378. 205-213 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M.Sugawara: "Factors Affecting Background Permeabilities of Ordered Mono-, Bi-, and Multilayrs as Channel Mimetic Sensing Membranes." Anal.Sci.10. 343-347 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.Odashima: "Chemical Sensing Based on Membrane Potential Change Induced by Host-Guest Complexation at Membrane Surface." Supramol.Chem.4. 101-113 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.Odashima: "Potentiometric Discrimination of Organic Amines by a Liquid Membrane Electrode Based on a Long Alkyl Chain Derivative of beta-Cyclodextrin." Mikrochim.Acta. 113. 223-238 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y.Umezawa: "Development of Biological and Synthetic Receptor-Embedded Lipid Membrare Assemblies with Molecular Recognition and Signal Transduction/Amplification Functions." New Functionality Materials B. 111-116 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] R.Naganawa: "Potentiometric Discrimination Between Planar and Octahedral Anionic Metal Cyano Complexes by Liquid Membrane Electrodes Based on Lipophilic Macrocyclic Dioxopolyamines." Electroanalysis. 5. 731-738 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.Umezawa: "Cation Permselectivity in the Phase Boundary of Ionophore-Incorporated Solvent Polymeric Membranes as Studied by Fourier Transform Infrared Attenuated Total Reflection Spectrometry." Anal.Chim.Acta. 282. 247-257 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.Odashima: "Voltammetric Study on a Condensed Monolayer of a Long Alkyl Cyclodextrin Derivative as a Channel Mimetic Sensing Membrane." Anal.Chem.65. 927-936 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.Odashima: "Potentiometric Discrimination of Organic Amines by a Liquid Membrane Electrode Based on a Lipophilic Hexaester of Calix [6] arene." Anal.Chem.65. 1074-1083 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S.Yoshiyagawa: "Optical Ion Sensor Based on Second-Harmonic Generation at the Surface of an Ionophore-Incorporated Poly (vinyl chloride) Liquid Membrane." Anal.Sci.9. 715-718 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] N.Sugao: "Na^+/D-Glucose Cotransporter Based Bilayer Lipid Membrane Sensor for D-Glucose." Anal.Chem.65. 363-369 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y.Adachi: "Na^+, K^+-ATPase-Based Bilayer Lipid Membrane Sensor for Adenosine 5'-Triphosphate." Anal.Chim.Acta. 281. 577-584 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.Tohda: "Liquid Membrane Electrodes for Nucleotides Based on Sapphyrin, Cytosine-Pendant Triamine and Neutral Cytosine Derivative as Sensory Elements." Sensors and Actuators B. 13-14. 669-672 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.Tohda: "Liquid Membrane Electrode for Guanosine Nucleotides Using a Cytosine-Pendant Triamine Host as the Sensory Element." Anal.Chem.64. 960-964 (1992)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-03-09   Modified: 2021-04-07  

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