1995 Fiscal Year Annual Research Report

救急医療をめぐる臨床法医中毒学的緊急薬毒物スクリーニング

Research Project

Project/Area Number	05404027
Research Institution	Kanazawa University
Principal Investigator	市村藤雄金沢大学, 医学部・附属病院, 教授 (40143911)
Co-Investigator(Kenkyū-buntansha)	高安達典金沢大学, 医学部, 助教授 (80154912) 吉田豊金沢大学, 医学部・附属病院, 助手 (30201023) 大島徹金沢大学, 医学部, 教授 (40183024) 橋本琢磨金沢大学, 医学部, 教授 (10124710) 永野耐造科学警察研究所, 所長 (20073679)
Keywords	救急医療 / 薬物動態学 / 精度管理 / 臨床法医学 / 法医中毒学 / 迅速スクリーニングツシステム / REMEDi‐HSシステム
Research Abstract	1.薬毒物動態の観点から腹膜透過性について検討し、11種類のバルビタール酸系薬剤の脂溶性と腹膜透過速度との間に相関関係が成り立つこと、並びにモノカルボン酸化合物の腹膜透過には特殊輸送系が存在する可能性を示唆する結果を得ている。現在、抗悪性腫瘍剤について検討中であるが,腹膜内に貯留傾向を示す薬剤や、腹膜に特殊輸送系が存在する可能性を示唆される薬剤についての研究結果も得られており、今後更に薬物の性状と腹膜透過性についての研究を進めてゆく予定である。 2.REMEDiシステム等を用いて,本年度は以下の点で新知見を得た. (1)REMEDiシステムの充実・改善:昨年度に引き続きフェニトロチオン等計4種の薬物をライブラリーに新規追加した.更にヒドロキシジン等計10種の薬物の検量線を作成し,同時的に定性・定量できるようにした.更に、REMEDiシステムのパラメーターも検討し、これにより薬物同定の信頼性の向上及び迅速定量が可能になった。 (2)ボランティアによる服用実験:ジフェンヒドラミンをボランティアに服用させ血液、尿を分析した.測定値がジフェンヒドラミンの検出限界値(40ng/ml)より高く、薬物治療モニタリングの可能性が明らかになり、更に測定値をGC-MSのそれと比較した所、高い相関が得られ、REMEDiシステム有用性が示された。 (3)覚醒剤に関する実験的検討:法医学的に重要な覚醒剤のメタンフェタミン及びアンフェタミンと、腐敗した試料にしばしば発現するフェネチルアミンとの識別が重要である。REMEDiシステムではフェネチルアミンとアンフェタミンは識別不可能であったが、メタンフェタミンとは十分識別可能であり、更に、検出限界値は150ng/mlで覚醒剤メタンフェタミンの分析が可能であった。

Research Products

(23 results)

All Other

All Publications (23 results)

[Publications] E.Nakashima et al.: "Systematic approach to a dosage regimen for phenytoin based on one‐point, steady‐state plasma concentration." Ther. Drug Monit.17. 12-18 (1995)
[Publications] E.Nakashima et al.: "Age‐related changes in pharmacokinetic parameters of phenytoin after liver resection in rats." Pharm. Res.12. 1530-1534 (1995)
[Publications] E.Nakashima et al.: "Reversal of drug sensitivity in MDR subline of P388 leukemia by gene‐targeted antisense oligonucleotide." J.Pharm. Sci.84. 1205-1209 (1995)
[Publications] E.Nakashima et al.: "Human MCAF gene transfer enhances the metastatic capacity of a mouse cachectic adenocarcinoma cell line in vivo." Pharm. Res.12. 1598-1604 (1995)
[Publications] E.Nakashima et al.: "Quantitative relationship between structure and peritoneal membrane transport based on physiological pharmacokinetic concepts for acidic drugs." Drug Metab. Dispos.23. 1220-1224 (1995)
[Publications] 西部万千子他: "ルミパス1200によるCA125測定の検討" 日本臨床検査自動化学会会誌. 20. 803-806 (1995)
[Publications] 橋本琢磨: "内分泌検査のQuick Return Service" 臨床病理. 43. 1034-1037 (1995)
[Publications] 橋本琢磨: "甲状腺機能検査法の臨床的評価" 日本医事新報. 3734. 21-25 (1995)
[Publications] A.Tomoda et al.: "Variation of urinary pH and bicarbonate conentrations of students in metropolitan and rural areas of Japan." Arch. Environ. Health.50. 457-461 (1995)
[Publications] T.Takayasu et al.: "Analysis of several voiatile anesthetics in biological samples by pulse heating method." Proc. 13th meeting Internatl. Assoc. Forensic Sciences. 5. 146-150 (1995)
[Publications] T.Takayasu et al.: "Screening and determination of methamphetamine and amphetamine in the blood, urine and stomach contents in emergency medical care and autopsy cases." J.Clin. Forensic Med.2. 25-33 (1995)
[Publications] K.Saito et al.: "Determination of the volatile anesthetics halothane, enflurane, isoflurane and sevoflurane in biological specimens by pulse‐heating GC‐MS." J.Anal. Toxicol.19. 115-119 (1995)
[Publications] T.Takayasu et al.: "Screening of volatile substances and determination of toluene (a thinner component)in the blood and urine in emergency medical and autopsy cases by pulse heating method." J.Clin. Forensic Med.2. 65-72 (1995)
[Publications] T.Takayasu et al.: "Postmortem degradation of administered ethanol‐d6 and production of endogenous ethanol‐Experimental studies using rats and rabbits." Forensic Sci. Int.76. 129-140 (1995)
[Publications] T.Takayasu et al.: "Experimental studies on postmortem diffusion of ethanol‐d6 using rats." Forensic Sci. Int.76. 179-188 (1995)
[Publications] 大辻雅彦他: "REMEDi‐HSシステムを用いた薬毒物分析" 法中毒. 13. 39-48 (1995)
[Publications] M.Ohtsuji et al.: "Screening of antihistamine agents (diphenhydramine)with blood and urine samples by REMEDi‐HS System." Proc. of the 13th International Conference on Alcohol, Drugs and Traffic Safety. 1. 321-326 (1995)
[Publications] K.Shibata et al.: "Severe hyponatremia causedby cement ingestion during carbamazepine therapy." Am.J.Emergency Med.13. 245-247 (1995)
[Publications] T.Ohshima et al.: "A case of death due to outbreak of fire during thinner abuse." Jpn.J.Legal Med.50. 33-36 (1996)
[Publications] E.Morishita et al.: "Hypercoagulability and high lipoprotein(a)levels in patients with type II diabetes mellitus." Atherosclerosis. 120. 7-14 (1996)
[Publications] E.Nakashima et al.: "Kinetic evidence for facllitated peritoneal transport of benzoic acid in rats." J.Pharm. Pharmacol.(in press). (1996)
[Publications] M.Ohtsuji et al.: "The use of REMEDi HS in emergency toxicology for a rapid estimate of drug concentrations in urine, serum and gastric samples." J.Forensic Sci.41(in press). (1996)
[Publications] 橋本琢磨: "RIAとIRMA 免疫実験操作法(右田俊介、紺田進、本庶佑、濱岡利之編)" 南江堂, 298-309 (1995)

1995 Fiscal Year Annual Research Report

救急医療をめぐる臨床法医中毒学的緊急薬毒物スクリーニング

Principal Investigator

市村 藤雄 金沢大学, 医学部・附属病院, 教授 (40143911)

Research Products

[Publications] E.Nakashima et al.: "Systematic approach to a dosage regimen for phenytoin based on one‐point, steady‐state plasma concentration." Ther. Drug Monit.17. 12-18 (1995)

[Publications] E.Nakashima et al.: "Age‐related changes in pharmacokinetic parameters of phenytoin after liver resection in rats." Pharm. Res.12. 1530-1534 (1995)

[Publications] E.Nakashima et al.: "Reversal of drug sensitivity in MDR subline of P388 leukemia by gene‐targeted antisense oligonucleotide." J.Pharm. Sci.84. 1205-1209 (1995)

[Publications] E.Nakashima et al.: "Human MCAF gene transfer enhances the metastatic capacity of a mouse cachectic adenocarcinoma cell line in vivo." Pharm. Res.12. 1598-1604 (1995)

[Publications] E.Nakashima et al.: "Quantitative relationship between structure and peritoneal membrane transport based on physiological pharmacokinetic concepts for acidic drugs." Drug Metab. Dispos.23. 1220-1224 (1995)

[Publications] 西部万千子 他: "ルミパス1200によるCA125測定の検討" 日本臨床検査自動化学会会誌. 20. 803-806 (1995)

[Publications] 橋本琢磨: "内分泌検査のQuick Return Service" 臨床病理. 43. 1034-1037 (1995)

[Publications] 橋本琢磨: "甲状腺機能検査法の臨床的評価" 日本医事新報. 3734. 21-25 (1995)

[Publications] A.Tomoda et al.: "Variation of urinary pH and bicarbonate conentrations of students in metropolitan and rural areas of Japan." Arch. Environ. Health.50. 457-461 (1995)

[Publications] T.Takayasu et al.: "Analysis of several voiatile anesthetics in biological samples by pulse heating method." Proc. 13th meeting Internatl. Assoc. Forensic Sciences. 5. 146-150 (1995)

[Publications] T.Takayasu et al.: "Screening and determination of methamphetamine and amphetamine in the blood, urine and stomach contents in emergency medical care and autopsy cases." J.Clin. Forensic Med.2. 25-33 (1995)

[Publications] K.Saito et al.: "Determination of the volatile anesthetics halothane, enflurane, isoflurane and sevoflurane in biological specimens by pulse‐heating GC‐MS." J.Anal. Toxicol.19. 115-119 (1995)

[Publications] T.Takayasu et al.: "Screening of volatile substances and determination of toluene (a thinner component)in the blood and urine in emergency medical and autopsy cases by pulse heating method." J.Clin. Forensic Med.2. 65-72 (1995)

[Publications] T.Takayasu et al.: "Postmortem degradation of administered ethanol‐d6 and production of endogenous ethanol‐Experimental studies using rats and rabbits." Forensic Sci. Int.76. 129-140 (1995)

[Publications] T.Takayasu et al.: "Experimental studies on postmortem diffusion of ethanol‐d6 using rats." Forensic Sci. Int.76. 179-188 (1995)

[Publications] 大辻雅彦 他: "REMEDi‐HSシステムを用いた薬毒物分析" 法中毒. 13. 39-48 (1995)

[Publications] M.Ohtsuji et al.: "Screening of antihistamine agents (diphenhydramine)with blood and urine samples by REMEDi‐HS System." Proc. of the 13th International Conference on Alcohol, Drugs and Traffic Safety. 1. 321-326 (1995)

[Publications] K.Shibata et al.: "Severe hyponatremia causedby cement ingestion during carbamazepine therapy." Am.J.Emergency Med.13. 245-247 (1995)

[Publications] T.Ohshima et al.: "A case of death due to outbreak of fire during thinner abuse." Jpn.J.Legal Med.50. 33-36 (1996)

[Publications] E.Morishita et al.: "Hypercoagulability and high lipoprotein(a)levels in patients with type II diabetes mellitus." Atherosclerosis. 120. 7-14 (1996)

[Publications] E.Nakashima et al.: "Kinetic evidence for facllitated peritoneal transport of benzoic acid in rats." J.Pharm. Pharmacol.(in press). (1996)

[Publications] M.Ohtsuji et al.: "The use of REMEDi HS in emergency toxicology for a rapid estimate of drug concentrations in urine, serum and gastric samples." J.Forensic Sci.41(in press). (1996)

[Publications] 橋本琢磨: "RIAとIRMA 免疫実験操作法(右田俊介、紺田進、本庶佑、濱岡利之編)" 南江堂, 298-309 (1995)

市村藤雄金沢大学, 医学部・附属病院, 教授 (40143911)

[Publications] 西部万千子他: "ルミパス1200によるCA125測定の検討" 日本臨床検査自動化学会会誌. 20. 803-806 (1995)

[Publications] 大辻雅彦他: "REMEDi‐HSシステムを用いた薬毒物分析" 法中毒. 13. 39-48 (1995)