1994 Fiscal Year Annual Research Report

慢性関節リウマチの病因遺伝子に関する研究

Research Project

Project/Area Number	05404028
Research Institution	St. Marianna University School of Medicine
Principal Investigator	西岡久寿樹聖マリアンナ医科大学, 難病治療研究センター, 教授 (60049070)
Co-Investigator(Kenkyū-buntansha)	丸山征郎鹿児島大学, 医学部・臨床検査医学講座, 教授 (20082282)
Keywords	アポトーシス / HTLV-I tax遺伝子 / 単クローン性滑膜細胞 / 抗Fas抗体
Research Abstract	HTLV-I tax遺伝子の病因遺伝子とその特性を次の点より明らかにした。 1)正常滑膜細胞にtax遺伝子を導入したところ強力な増殖能を獲得するとともに正常の細胞増殖に必要なプロトオンコジーンやサイトカイン遺伝子の発現を誘導した。 2)tax遺伝子を導入した滑膜細胞の系においてclass II分子及びCTLの誘導が確認された。この事実はtax遺伝子が滑膜細胞を免疫の標的細胞に誘導することを初めて明らかにしたものである。 3)RA滑膜細胞や関節滑膜の浸潤T細胞に抗Fas抗体の感受性が存在することを明らかにした。 4)RAの滑膜固有細胞に単クローン性の細胞集団が存在することを明らかにしその細胞株を樹立した。

Research Products
(11 results)

All Other

All Publications (11 results)

[Publications] Dohi-M,Yamamoto-K,et.al.: "Accumulation of multiple T cell clonotypes in lungs of healthy individuals and patients with pulmonary sarcoidsis." J.Immunol.152. 1983-1988 (1994)
[Publications] Hasunuma-T,Nakajima-T,et.al.: "Establishment and characterzation of synovial cell clones with integrated human T-cell leukemia virus type-I." Clin.Immunol.Immunopathol.72. 90-97 (1994)
[Publications] Yagi-J,Nakata-M,et.al.: "Superantigen-like properties of the bispecific antibody specific for MHC class II molecule and V βdomain of the T cell antigen receptor." J.Immunol.152. 3833-3841 (1994)
[Publications] Okubo-M,Kurokawa-M,et.al.: "Clonotype analysis of the peripheral blood T cell and the autoantigenreactive T cells from patients with mixed connective tissue disease." J.Immunol.153. 3784-3790 (1994)
[Publications] Kato-T,Suzuki-S,et.al.: "An improved bacterial expression vector producing a thrombincleavable fusion protein." Mol.Biol.13. 85-88 (1994)
[Publications] Kato-T,Suzuki-S,Nishioka-K,Yamamoto-K.: "A method to construct a gene that encodes repetitive amino acid sequence by PCR." Anal.Bio.Chem.220. 428-428 (1994)
[Publications] Kato,T.,Suzuki,S.,et.al.: "Comparison of the J beta gene among different T cell receptor V beta familiesin spleens of C57BL/6 mice." Eur.J.Immunol.24. 2410-2414 (1994)
[Publications] Miura,H.,Yamanaka,H.,et.al.: "Identification of commonly recognized epitopes on a rare autoantigen:poly(ADP-ribose)polymerase." Clin.Immunol.Immunopathol.73. 290-295 (1994)
[Publications] Nakajima,T.,Aono,H.,et.al.: "Apoptosis and functional fas antigen in rheumatoid Arthritis synoviocytes" Arthritis Rheum.(in press).
[Publications] Yamamoto,K.,Aono,H.,et.al.: "Oligoclonal proliferation of human T-cell leukemia virus type I infected lymphocytes in lesions of virus-induced arthropathy." Bio.Bio.Res.Commun.(in press).
[Publications] 和田攻、大内尉義、木佐木友成編: "最新の慢性関節リウマチ診療" メディカル葵出版, 141 (1995)

1994 Fiscal Year Annual Research Report

慢性関節リウマチの病因遺伝子に関する研究

Principal Investigator

西岡 久寿樹 聖マリアンナ医科大学, 難病治療研究センター, 教授 (60049070)

Research Products

[Publications] Dohi-M,Yamamoto-K,et.al.: "Accumulation of multiple T cell clonotypes in lungs of healthy individuals and patients with pulmonary sarcoidsis." J.Immunol.152. 1983-1988 (1994)

[Publications] Hasunuma-T,Nakajima-T,et.al.: "Establishment and characterzation of synovial cell clones with integrated human T-cell leukemia virus type-I." Clin.Immunol.Immunopathol.72. 90-97 (1994)

[Publications] Yagi-J,Nakata-M,et.al.: "Superantigen-like properties of the bispecific antibody specific for MHC class II molecule and V βdomain of the T cell antigen receptor." J.Immunol.152. 3833-3841 (1994)

[Publications] Okubo-M,Kurokawa-M,et.al.: "Clonotype analysis of the peripheral blood T cell and the autoantigenreactive T cells from patients with mixed connective tissue disease." J.Immunol.153. 3784-3790 (1994)

[Publications] Kato-T,Suzuki-S,et.al.: "An improved bacterial expression vector producing a thrombincleavable fusion protein." Mol.Biol.13. 85-88 (1994)

[Publications] Kato-T,Suzuki-S,Nishioka-K,Yamamoto-K.: "A method to construct a gene that encodes repetitive amino acid sequence by PCR." Anal.Bio.Chem.220. 428-428 (1994)

[Publications] Kato,T.,Suzuki,S.,et.al.: "Comparison of the J beta gene among different T cell receptor V beta familiesin spleens of C57BL/6 mice." Eur.J.Immunol.24. 2410-2414 (1994)

[Publications] Miura,H.,Yamanaka,H.,et.al.: "Identification of commonly recognized epitopes on a rare autoantigen:poly(ADP-ribose)polymerase." Clin.Immunol.Immunopathol.73. 290-295 (1994)

[Publications] Nakajima,T.,Aono,H.,et.al.: "Apoptosis and functional fas antigen in rheumatoid Arthritis synoviocytes" Arthritis Rheum.(in press).

[Publications] Yamamoto,K.,Aono,H.,et.al.: "Oligoclonal proliferation of human T-cell leukemia virus type I infected lymphocytes in lesions of virus-induced arthropathy." Bio.Bio.Res.Commun.(in press).

[Publications] 和田 攻、大内尉義、木佐木友成 編: "最新の慢性関節リウマチ診療" メディカル葵出版, 141 (1995)

西岡久寿樹聖マリアンナ医科大学, 難病治療研究センター, 教授 (60049070)

[Publications] 和田攻、大内尉義、木佐木友成編: "最新の慢性関節リウマチ診療" メディカル葵出版, 141 (1995)