1994 Fiscal Year Final Research Report Summary
Study on Bioreactor with Immobilized mRNA for protein Production
| Project/Area Number |
05453110
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
生物・生体工学
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| Research Institution | Tokyo Institute of Technology |
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Principal Investigator |
AIZAWA Masuo Tokyo Inst.of Tech., Dept.of Bioengine.Proffesor, 生命理工学部, 教授 (00016742)
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| Co-Investigator(Kenkyū-buntansha) |
KOBATAKE Eiry Tokyo Inst.of Tech., Dept.of Bioengine.Lecture, 生命理工学部, 講師 (00225484)
KOBATAKE Eiry Tokyo Inst.of Tech., Dept.of Bioengine.Lecture (00225484)
KOBATAKE Eiry Tokyo Inst.of Tech., Dept.of Bioengine.Lecture (00225484)
KOBATAKE Eiry Tokyo Inst.of Tech., Dept.of Bioengine.Lecture (00225484)
KOBATAKE Eiry Tokyo Inst.of Tech., Dept.of Bioengine.Lecture (00225484)
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| Project Period (FY) |
1993 – 1994
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| Keywords | Cell free protein production / Reactor / Protein A / Luciferase |
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| Research Abstract |
In the first year of the project, protein A was produced by a cell free protein synthesis system which we developed. An important characteristic of our system is that a mRNA is immobilized on the beads through the end of mRNA chain. In the result, it was proved that such the immobilized mRNA has a high resistance for mRNA degradation enzymes.
In the last year of the project, we investigated the repetition use of immobilized mRNA on the production of luciferase. Production profile of the luciferase system is able to observed by monitoring the luminescence of produced luciferase.
In conclusion, unstability of mRNA in production cell can be largely overcome by the immobilization of mRNA.All the results of our project suggest that the cell free protein production system could utilized for commercially protein production.
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