1994 Fiscal Year Final Research Report Summary
Study on the structure-function correlation of CO_2-fixation enzymes toward the industrial application
| Project/Area Number |
05453122
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
工業物理化学
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| Research Institution | Osaka University |
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Principal Investigator |
KAI Yasushi Osaka University Fuculty of Engineering Professor, 工学部, 教授 (40029236)
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| Co-Investigator(Kenkyū-buntansha) |
INOUE Tsuyoshi Osaka University Fuculty of Engineering Research Assistant, 工学部, 助手 (20263204)
KANEHISA Nobuko Osaka University Fuculty of Engineering Research Assistant, 工学部, 助手 (20177538)
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| Project Period (FY) |
1993 – 1994
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| Keywords | X-ray structure analysis / CO_2-fixation enzymes / spinach / 2.0 A resolution / RuBisCo / phosphoenolpyrubicacidcarboxylase (PEPC) / reflection-intensity-measurement |
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| Research Abstract |
The spinach RuBisCO (ribulose-1,5-bisphosphate carboxylase oxygenase) were purified and crystallized by the use of polyethylene glycol as a salt for salting out during the purification process and a precipitant for crystallization. The X-ray diffraction data were measured by the use of synchrotron radiation in Tsukuba KEK.The refinement of the crystal structure was proceeded up to the current R value of 19.0% for 2.0 ? 81 resolution data. The comparison of the molecular structure obtained by the present work with the spinach RuBisCO obtained by use of ammonium sulfate as a salt and precipitant are under investigation. The difference in the enzymatic function between these RuBisCO is expected to understand from the structure-function correlation based on the high resolution molecular structures.
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[Publications] T.Inoue, N.Shibata, H.Nakanishi, S.Koyama, H.Ishii, Y.Kai, S.Harada, N.Kasai, Y.Ohshiro, S.Suzuki, T.Kohzuma, K.Yamaguchi, S.Shidara and H.Iwasaki: "Structure of Azurin from Achromobacter xylosoxidans NCIB11015 at 2.5? 81 Resolution" J.Biochem.116. 1193-1197 (1994)
Description
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