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1994 Fiscal Year Final Research Report Summary

Propeties of Native Cellulose II with Folded-Chain Structure

Research Project

Project/Area Number 05453175
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 生物資源科学
Research InstitutionThe University of Tokyo

Principal Investigator

KUGA Shigenori  The University of Tokyo, Assistant professor, 農学部, 助手 (60012051)

Co-Investigator(Kenkyū-buntansha) KONDO Tetsuo  Forestry & Forest Products Research Institute, Research Associate, 研究員
ISOGAI Akira  The University of Tokyo, Associate professor, 農学部, 助教授 (40191879)
Project Period (FY) 1993 – 1994
KeywordsAcetobater / Native band cellulose / Cellulose II / Folded-chaincrystal / Level-off degree of polymerization / Solid-state NMR
Research Abstract

(1) Culture conditions for the native band-producing strain were established. The bulk material gave the X-ray diffraction pattern of well defined cellulose II.
(2) Leveling-off behavior on acid hydrolysis of native band cellulose was similar to those of other cellulose samples, but the former's leveling-off value (LODP) (18.4) was remarkably lower than others. When the native band cellulose was dissolved in and regenerated from concentrated alkali, its cahracteristic band morphology was lost and LODP changed to ca.50, a value similar to ordinary regenerated celluloses. These results strongly support the proposed model of regular chain folding in the native band with a periodicity of 10 nm.
(3) The typical thickness of native band was determined by shadowing technique as ca.10 nm.
(4) Analysis of the change in solid-state 13C NMR spectrum by acid hydrolysis of native band showed that the chain folding region involved irregular conformations of glucose residues, whose contribution to the spectrum coinciding with that of amorphous regions of other celluloses.
(5) These findings lead to a model of native band cellulose consisting of strand-like structures each composed of ca.16 glucan chains forming a monomolecular sheet, which is subsequently folded regularly.

  • Research Products

    (8 results)

All Other

All Publications (8 results)

  • [Publications] S.Shibazaki,et al.: "Acid hydrolysis behavior of microbial cellulose II." Polymer. 34. 4971-4976 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 空閑重則: "セルロースの生合成はどこまで分ったか" Cellulose Communications. 1. 9-14 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] A.Isogai et al.: "Solid-state CP/MAS 13C-NMR Analysis of cellulose & tri-O-substituted cellulose ethers" Carbohydr.,Polymers. 21. 277-281 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Kondo et al.: "Hydrogen bonds in regioselectively substituted cellulose derivatives" J.Polym.Sci.32. 1229-1236 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shibazaki H,Kuga S,Onabe F,Brown RM Jr.: "Acid hydrolysis behavior of microbial cellulose" Polymer. 34. 4971-4976 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kuga S: "Recent progresses in cellulose biosynthesis study" Cellul.Comm.1. 9-14 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Isogai A,Kato T,Uryu T,Atalla RH: "Solidstate CP/MAS 13C-NMR analysis of cellulose and tri-O-substituted cellulose ethers" Carbohydr.Polym.21. 277-281 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kondo T: "Hydrogen bonds in regioselectively substituted cellulose derivatives" J.Polym.Sci.32. 1229-1236 (1994)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-03-09  

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