1994 Fiscal Year Final Research Report Summary
Development of Method for Evaluating Safety of Water Applying PCR
| Project/Area Number |
05453200
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
環境保全
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| Research Institution | THE UNIVERSITY OF TOKYO |
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Principal Investigator |
OHGAKI Shinichiro University of Tokyo, Faculty of Engineering, Department of Urban Engineering, Professor, 工学部, 教授 (20005549)
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| Co-Investigator(Kenkyū-buntansha) |
KAMIKO Naoyuki University of Tokyo, Faculty of Engineering, Department of Urban Engineering, Re, 工学部, 助手 (70251345)
YAMAMOTO Kazuo University of Tokyo, Faculty of Engineering, Department of Urban Engineering, As, 工学部, 助教授 (60143393)
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| Project Period (FY) |
1993 – 1994
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| Keywords | PCR / RNA phage / MPN / Indicator for viruses / Ultraviolet Disinfection / Monitoring |
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| Research Abstract |
PCR method was developed as a quantitative detection of viruses in water, including the investigation of pre-treatment method. Application of PCR method to the water treated by a treatment process (Ultraviolet Irradiation). RNA phage Q-beta was used for the experiment as a model virus.
In order to inprove the quantitatative PCR,optimisation of beffer concentration in both reverse transcription and DNA amplify, and development of nested PCR were done. Result showed the detection limit was 1 PFU.It was suggested that using computer program to calculate MPN lead to the utilization of quantitative PCR date.
Effect of ultraviolet disinfection on the PCR detection was also investigated. Value of surviving phage concentration measured by conventional plapue method and that measured by PCR method were compared. Result showed that PCR measurment detected most of inactivated phages. Quantity of detection was assumed to depend on the ratio of the length of target RNA of PCR detection to the whole lengh of phage RNA.
These results showed that PCR method was hard to be applied to the monitoring the performance of ultraviolet disinfection. Further research was required to utilize the PCR to measure the safety of water.
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