• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

1995 Fiscal Year Final Research Report Summary

Molecular mechanisms of cytoplasmic division in Tetrahymena using cell-division-arrest mutants

Research Project

Project/Area Number 05454025
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 動物生理・代謝
Research InstitutionJoubu University (1994-1995)
University of Tsukuba (1993)

Principal Investigator

WATANABE Yoshio  Joubu University, Department of Commercial Sciences, Professor, 商学部, 教授 (00015918)

Co-Investigator(Kenkyū-buntansha) NUMATA Osamu  University of Tsukuba, Institute of Biological Sciences, Associate Professor, 生物科学系, 助教授 (50189354)
Project Period (FY) 1993 – 1995
KeywordsTetrahymena / cytoplasmic division / cell-division-arrest mutant / p85 gene / F-actin bundling factor / EF-1alpha / Ca^<2+> / calmodulin / calmodulin family protein
Research Abstract

To elucidate the molecular mechanisms of cytoplasmic division in animal cells, we have mainly investigated the roles of mutant gene products of temperature-sensitive cell-division-arrest mutants (cda loci) in ciliated protozoan Tetrahymena.
Tetrahymena cdaA mutant has a defect in the detemination factor of division plane. We demonstrated that the factor was responsible not only for division plane determination but also for the formation of contractile ring microfilaments as a polymerization nucleus. The mutant gene product has been shown to be a 85 kDa protein (designated as p85). In this research period. we succeeded in cloning and sequencing of cDNA for p85. The data base analysis indicates that p85 is a new protein different from proteins known so far, but sharespartly homologous sequences with actin, EF-1alpha, Ca^<2+>/calmodulin-dependent protein kinase, suggesting that p85 is a multifunctional protein playing crucial roles in cell division.
Tetrahymena cdaC mutant is relevant to the direct mechanism of division furrow constriction and the mutant gene product has been shown to be an F-actin bundling factor. In this regard, we proved that Tetrahymena EF-1alpha has remarkable F-actin bundling activity in a physiological condition. Moreover, we demonstrated that the F-actin bundling activity was clearly regulated by Ca^<2+>/calmodulin system.
Concerning the Ca^<2+>-regulation in the constriction of contracatile ring by actomyosin system, we have investigated the functions in cell division of the threo kinds of Tetrahymena calmodulin family proteins, such as calmodulin, TCBP-25, TCBP-23, by purifying these proteins after expressing their genes in E.coli.

  • Research Products

    (28 results)

All Other

All Publications (28 results)

  • [Publications] Katoh,M.,Hirono,M.,Takemasa,T.,Kimura,M.& Watanabe,Y.: "A micronuclear-specific sequence exists in the 5′-upstream region of calmodulin gene in Tetrahymena thermophila." Nucl.Acids Res.21. 2409-2414 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Takagi,T.Iwasa,Yuasa,Shikama,Takemasa,Watanabe: "Primary structure of Tetrahymena hemoglobins." Biochim.Biophys.Acta. 1173. 75-78 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nagata,K.,Takemasa,Alam,Hattori,Watanabe,Nozawa: "Cloning of cDNA encoding a cell-cycle-regulatory GTP-binding low-Mr (GBLM)protein from Tetrahymena thermophila and Tetrahymena pyriformis." Gene. 144. 123-125 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Numata,O.,Suzuki H.,Ohba,H. & Watanabe,Y.: "Yhe mutant gene product of a Tetrahymena cell-division-arrest mutant cdaA ia localized in the accessory structure of specialized basal body close to division furrow." Zool.Sci.12. 133-135 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Takeda,T.,Kurasawa,Watanabe,Numata,o.: "Polymerization of highly puritied Tetrahymena 14-nm filament protein/citrate synthase into filaments and its possible role in requlation of enzymatic activity" J.Biochem. 117. 869-874 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kojima,H.,Chiba,Watanabe,Y. & Numata,O.: "Citrate synthase purified from Tetrahymena mitochondria is identical to Tetrahymena 14-nm filament protein." J.Biochem.118. 189-195 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nunata,O.,Yasada,T. & Watanabe,Y.: "Tetrahymena intramitochondrial filamentous inclusions contain Tetrahymena 14-nm filament protein." Exp.Cell Res.218. 123-131 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hanyu,K.,Takenasa Numata,Takahashi & Watanabe,Y.: "Immunofluorescence iocalization of a 25 kDa Tetrahymena EF-hand Ca^<2+>-binding protein,TCBP-25,to the cell cortex,and possible involvement in conjugation." Exp.Cell Res.219. 487-493 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kurasawa,Y.,Hanyu,K.,Watanabe,Y. & Numata,O.: "F-actin bundling activity of Tetrahymena elongation factor 1α is regulated by Ca^<2+>/calmodulin." J.Biochem.In Press. (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hanyu,K.,Numata,O.,Takahashi,M. & Watanabe,Y.: "Immunofluorescence localization of a 23 kDa Tetrahymena calcium-binding protein,TCBP-23,in the cell cortex." J.Biochem.In Press. (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 渡辺良雄: "繊毛虫の分子生物学" 蛋白質・核酸・酵素. 39. 101-105 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 沼田治・保田友義・渡辺良雄: "原生動物テトラヒメナの細胞質分裂" 細胞. 26. 175-180 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Watanabe,Y. & Berger,J.D.: "“Control of cell Cycle"In Progress in Protozoology,eds.by Hausmann & Hulsmann" Gustay Fischer Verlag,Stuttgart, 97-98 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Watanabe,Y.,Numata,O.,Kurasawa,Y. & Katoh,M.: "“Cultivation of Tetrahymena cells",In Cell Biology: A Laboratory Handbook,ed.by J.E.Celis,Vol.I" Academic Press,San Diego, 389-404 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Watanabe,Y.,Hanyu,K.,Takemasa,T. & Numata,O.: "“TCBP-25" In Guidebook to the Calcium Binding Protein,ed.by M.R.Celio" Oxfrod University Press, 85-88 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Katoh, M., Hirono, M., Takemasa, T., Kimura, M.& Watanabe, Y.: "A micronuclear-specific sequence exists in the 5'-upstream region of calmodulin gene in Tetrahymena thermophila." Nucl.Acids Res.21. 2409-2414 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takagi, T., Iwasa, H., Yuasa, H., Shikama, K., Takemasa, T.& Watanabe, Y.: "Primary structure of Tetrahymena hemoglobins." Biochim.Biophys.Acta. 1173. 75-78 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Watanabe, Y.& Berger, J.D.: "Control of cell cycle." In Progress in Protozoology, eds.by K.Hausmann and N.Hulsmann, Gustav Fischer Verlag, Stuttgart. 97-98 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nagata, K., Takemasa, T., Alam, S., Hattori, T., Watanabe, Y.& Nozawa, Y.: "Cloning of cDNAs encoding a cell-cycle-regulatory GTP-binding low-Mr (GBLM) protein, Ran/TC4, from micronucleated Tetrahymena thermophila and amicronucleated Tetrahymena pyriformis." Gene. 144. 123-125 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Watanabe, Y., Numata, O., Kurasawa, Y.& Katoh, M.: "Cultivation of Tetrahymena cells." In Cell Biology : A Laboratory Handbook, ed, by J.E.Celis, Academic Press, San Diego.Vol.I. 389-404 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Numata, O., Suzuki, H., Ohba, H,& Watanabe, Y.: "The mutant gene product of a Tetrahymena cell-division-arrest mutant cdaA is localized in the accessory structure of specializad basal body close to the division furrow." Zool.Sci.12. 133-135 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takeda, T., Kurasawa, Y., Watanabe, Y.& Numata, O.: "Polymerization of highly purified Tetrahymena 14-nm filament protein/citrate synthase into filaments and its possible role in regulation of enzymatic activity." J.Biochem.117. 869-874 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kojima, H., Chiba, J., Watanabe, Y.& Numata, O.: "Citrate synthase purified from Tetrahymena mitochondria is identical to Tetrahymena 14-nm filament protein." J.Biochem.118. 189-195 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Numata, O., Yasuda, T.& Watanabe, Y.: "Tetrahymena intramitochondrial filamentous inclusions contain Tetrahymena 14-nm filament protein/citrate synthase." Exp, Cell Res.218. 123-131 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hanyu, K., Takemasa, T., Numata, O., Takahashi, M.& Watanabe, Y.: "Immunofluorescence localization of a 25 kDa Tetrahymena EF-hand Ca^<2+>-binding protein, TCBP-25, to the cell cortex and possible involvement in conjugation." Exp.Cell Res.219. 487-493 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Watanabe, Y., Hanyu, K., Takemasa, T.& Numata, O.: "TCBP-25." In Guidebook to the Calcium Binding Proteins, ed, by M.R.Celio, Oxford University Press.85-88 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kurasawa, Y., Hanyu, K., Watanabe, Y.& Numata, O.: "F-actin bunndling activity of Tetrahymens elongation factor 1alpha is regulated by Ca^<2+>/calmodulin." J.Biochem.(In press). (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hanyu, K., Numata, O., Takahashi, M.& Watanabe, Y.: "Immunofluorescence localization of a 23 kDa Tetrahymena calcium-binding protein, TCBP-23, in the cell cortex." J.Biochem.(in press). (1996)

    • Description
      「研究成果報告書概要(欧文)」より

URL: 

Published: 1997-03-04  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi