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1995 Fiscal Year Final Research Report Summary

Comparative Study on Molecular Mechanism of Photoreception in the Retina and Pineal Gland

Research Project

Project/Area Number 05454027
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 動物生理・代謝
Research InstitutionThe University of Tokyo

Principal Investigator

FUKADA Yoshitaka  The University of Tokyo, Graduate School of Science, Professor, 大学院・理学系研究科, 教授 (80165258)

Co-Investigator(Kenkyū-buntansha) SHICHIDA Yoshinori  Kyoto University, Graduate School of Science, Associate Professor, 大学院・理学研究科, 助教授 (60127090)
Project Period (FY) 1993 – 1995
KeywordsRod / Cone / Pineal Gland / Photon-Signal Transduction / Photoreceptor Protein / Pinopsin / G-Protein / Transducin
Research Abstract

Many vertebrates have several types of photoreceptive cells ; rods and cones in the retina, and pinealocytes in the pineal gland. It is important to understand the difference in molecular mechanism responsible for the diverged cellular functions ; that is, rods for scotopic vision, cones for color vision, and pinealocytes for circadian regulation of biological clock. This project aimed at comparative studies on the photon-signal transduction processes in the three types of photoreceptive cells, particularly at the structures and functions of photoreceptive pigments and signal-transducing G-proteins in the cells.
[Rod] To identify the physiological roles of lipid modifications of transducin alpha-and betagamma-subunits (Talpha and Tbetagamma), we studied the mechanism of the subunit interaction and the membrane association. It ws clearly shown that the isoprenyl and methyl groups of Tgamma regulate the efficiency of photon-signal transduction by affecting 1) Talpha-Tbetagamma coupling th … More rough direct contact with alpha-subunit, and 2) the interaction between transducin and cellular membranes. Monoclonal antibodies recognizing the structure of the N-terminal fatty acid of Talpha stained almost all of the rod outer segments in a bovine retina, suggesting that a single rod cell contains the four isoforms of Talpha modified with four kinds of fatty acids. The target of recoverin was shown to be rhodopsin kinase. The complex of calcium-recoverin-rhodopsin kinase was stabilized by the membrane association, by which the kinase activity was effectively inhibited.
[Cone] Comparative studies on cone and rod photoreceptive pigments by low temperature spectroscopy, laser flash photolysis, and circular dichroism showed that the life times of the active intermediates of cone pigments are much faster than that of rhodopsin. This clearly indicates that the molecular property of cone pigment is one of the major determinants for physiological photoresponse of cones. Photochemical reactions of chimeric mutants composed of rod-and cone-type pigments demonstrated that the structures of helices 1 through 3 determines the life time of the active intermediate of photoreceptive pigments.
[Pinealocytes] Little is known about the photon-signal transduction process in a photosensitive pineal gland. We found opsin-like immunoreaction in the gland by immunohistochemistry. Then we isolated the cDNA encoding a pineal specific opsin-like protein. Reconstitution experiment with 11-cis-retinal revealed its blue-light sensitivity, and thus we named it "pinopsin" after pineal opsin. This is the first demonstration of an opsin expressed in non-retinal tissues. Less

  • Research Products

    (74 results)

All Other

All Publications (74 results)

  • [Publications] Daisuke Kojima: "Purification and low temperature spectroscopy of gecko visual pigments green and blue." Biochemistry. 34. 1096-1106 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kamon Sanada: "Role of heterogeneous N-terminal acylation of recoverin in rhodopsin phosphorylation." J.Biol.Chem.270. 15459-15462 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Rika Morishita: "Primary structure of a γ subunit of G protein, γ12, and its phosphorylation by protein kinase C." J.Biol.Chem.270. 29469-29475 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Toshiyuki Okano: "Molecular basis for tetrachromatic color vision." Comp.Biochem.Physiol.112B. 405-414 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Daisuke Kojima: "Molecular properties of chimerical mutants of Gecko blue and bovine rhodopsin." Biochemistry. (in press). (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Osamu Shono: "Preparation and characterization of monoclonal antibodies specific for lauroylated isoform of bovine transducin α-subunit." J.Neurochem.(in press). (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 深田吉孝: "生物時計の時刻合わせをする脳内タンパク質" 生物の科学 遺伝. 49. 8-9 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 深田吉孝: "分子レベルにおける生物時計機構の解析" 生化学. 67. 1137-1142 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 岡野俊行: "脳は光を感じる「第三の目」" ニュートン. 15. 102-107 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 深田吉孝: "視物質の多様化と視覚の進化" 細胞工学. 14. 1007-1014 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 岡野俊行: "光受容タンパク質の分子進化" 生物の科学 遺伝. 49. 28-34 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 深田吉孝: "光受容体の構造と機能調節" 実験医学. 14. 63-67 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 真田佳門: "シグナル伝達蛋白質における脂肪修飾の役割" 膜. (印刷中). (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kamon Sanada: "Unique lipids and unique of properties retinal proteins." Behavioral and Brain Sciences. 18. 486-487 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yoshitaka Fukada: "Effects of carboxyl methylation of photoreceptor G protein Y-subunit in visual transduction." J.Biol.Chem.269. 5163-5170 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Steven W.Lin: "What makes red visual pigments red? A resonance Raman microprobe study of retinal chromophore structure in iodopsin." Biochemistry. 33. 2151-2160 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hiroko Masuda: "Visual pigments in the pineal complex of the Japanese quail,Japanese grass lizard and bullforg: Immunocytochemistry and HPLC analysis." Tissue & Cell. 26. 101-113 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tetsuji Okada: "Circular dichroism of metaiodopsin II and its binding to transducin:A comparative study between meta II intermediates of iodopsin and rhodopsin." Biochemistry. 33. 4940-4946 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yoshinori Shichida: "Is chicken green-sensitive cone visual pigment a rhodopsin-like pigment?" Biochemistry. 33. 9040-9044 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Satoshi Tamotsu: "Localization of iodopsin and rod-opsin immunoreactivity in the retina and pineal complex of the river lamprey,Lampetra japonica." Cell Tissue Res.278. 1-10 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Toshiyuki Okano: "Pinopsin is a chicken pineal photoreceptive molecule." Nature. 372. 94-97 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tomoko Yoshikawa: "Immunoreactivities to rhodopsin and rod/cone transducin antisera in the retina,pineal complex and deep brain of the bullfrog,Rana catesbeiana." Zool.Sci.11. 675-680 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Takahiko Matsuda: "Characterization of interactions between transducin α/β γ-subunits and lipid membranes." J.Biol.Chem.269. 30363-30358 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yoshinori Shichida: "Nanosecond laser photolysis of iodopsin,a chicken red-sensitive cone visual pigment." Biochemistry. 32. 10832-10838 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Koichi Kokame: "Identification of the α-subunits of rod and cone transducin in chicken photoreceptor" Exp.Eye Res.57. 135-140 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tomiko Asano: "Purification of four forms of the βγ subunit complex of G proteins containing different γ subunits." J.Biol.Chem.268. 20512-20519 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kimihiko Kameyama: "Activation by G protein βγ subunits of β-adrenergic and muscarinic receptor kinase." J.Biol.Chem.268. 7753-7758 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 深田吉孝: "ピノプシンによる光シグナル伝達と生物時計.Newメディカルサイエンス『細胞内シグナル伝達のしくみ』" 羊土社(印刷中), (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 深田吉孝: "ニワトリ錐体および桿体視物質の抽出と精製.生物薬学科学実験講座9巻『ホルモン・生理活性物質II』" 廣川書店, 502(378-395) (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yoshitaka Fukada: "Methods in Enzymology: “Lipid Modifications of Proteins"(Casey,P.J.and Buss,J.E.,eds),担当項名:Prenylation and carboxylmethylation of G protein γ subunit." Academic Press, 15 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 深田吉孝: "日本薬理学雑誌、103巻 特集『三量体Gタンパク質研究の現状』、担当項名:三量体Gタンパク質の脂質修飾." 日本薬理学会, 10 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 岡野俊行: "生物科学ニュース、278、脳内光受容体ピノプシンと生物時計." 生物科学ニュース社, 3 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 深田吉孝: "蛋白質・核酸・酵素、G蛋白質の多様な脂質修飾とその意義." 共立出版, 14 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 深田吉孝: "生物薬科科学実験講座IV-4巻『生体膜の構造と機能〜GTP結合蛋白質と膜酵素活性〜』担当項目:視細胞トランスデューシン." 廣川書店(印刷中), (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 深田吉孝: "視覚の分子生物学.図説・分子病態学" 中外医学者, 553(400-409) (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 深田吉孝: "アデニル酸シクラーゼ.タンパク質化学講座第4巻『酵素-リアーゼ』" 廣川書店(印刷中), (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yoshitaka Fukada: "“Frontiers of Photobiology"(A.Shima ct al.,eds.)担当項名:Roles of lipids linked to α-and γ-subunits of photoreceptor G protein." Elsevier, 6 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] D.Kojima, H.Imai, T.Okano, Y.Fukada, F.Crescitelli, T.Yoshizawa and Y.Shichida: "Purification and low temperature spectroscopy of gecko visual pigments green and blue." Biochemistry. 34. 1096-1106 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.Sanada, T.Takao, Y.Shimonishi, T.Yoshizawa and Y.Fukada.: "Role of heterogeneous N-terminal acylation of recoverin in rhodopsin phosphorylation." J.Biol.Chem.270. 15459-15462 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] R.Morishita, H.Nakayama, T.Isobe, T.Matsuda, Y.Hashimoto, T.Okano, Y.Fukada, K.Mizuno, S.Ohno, O.Kozawa, K.Kato and T.Asano.: "Primary structure of a gamma subunit of G protein, gamma_<12>, and its phosphorylation by protein kinase C." J.Biol.Chem.270. 29469-29475 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Okano, Y.Fukada and T.Yoshizawa.: "Molecular basis for tetrachromatic color vision." Comp.Biochem.Physiol.112B. 405-414 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] D.Kojima, T.Oura, O.Hisatomi, F.Tokunaga, Y.Fukada, T.Yoshizawa and Y.Shichida.: "Molecular properties of chimerical mutants of Gecko blue and bovine rhodopsin." Biochemistry. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] O.Shono, K.Kokame, M.Araki, T.Takao, Y.Shimonishi, M.Murata, T.Yoshizawa and Y.Fukada.: "Preparation and characterization of monoclonal antibodies specific for lauroylated isoform of bovine transducin alpha-subunit : Immunohistochemical analysis of bovine retinas." J.Neurochem.(in press).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y.Fukada and T.Okano: "Brain photoreceptor resetting a biological clock." THE IDEN. 49. 8-9 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y.Fukada and T.Okano: "Molecular analysis of biological clock mechanism." SEIKAGAKU. 67. 1137-1142 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Okano and Y.Fukada: "Brain is the third eye." Newton. 15. 102-107 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y.Fukada and T.Okano: "Evolution of vision inferred from variety of visual pigments in vertebrates." Cell Technology. 14. 1007-1014 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Okano and Y.Fukada: "Molecular evolution of the photoreceptive proteins." THE IDEN. 49. 28-34 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y.Fukada and K.Sanada: "Structure and functional regulation of photoreceptors." EXPERIMENTAL MEDICINE. 14. 63-67 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.Sanada and Y.Yoshitaka: "Lipid modifications of signal-transducing proteins." Membrane. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.Sanada and Y.Fukada: "Unique lipids and unique properties of retinal proteins." Behavioral and Brain Sciences. 18. 486-487 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y.Fukada and T.Okano: "Photon-signal transduction mediated by pinopsin and its implication in biological clock." New Medical Science. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y.Fukada: "Extraction and purification of chicken cone and rod visual pigments." Experimental Manuals for Bio-Pharmaceutical Sciences. Vol.18. 378-395 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y.Fukada, T.Matsuda, K.Kokame, T.Takao, Y.Shimonishi, T.Akino and T.Yoshizawa: "Effects of carboxyl methylation of photoreceptor G protein gamma-subunit in visual transduction." J.Biol.Chem.269. 5163-5170 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S.W.Lin, Y.Imamoto, Y.Fukada, Y.Shichida, T.Yoshizawa and R.A.Mathies: "What makes red visual pigments red? A resonance Raman microprobe study of retinal chromophore structure in iodopsin." Biochemistry. 33. 2151-21260 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H.Matsuda, T.Oichi, M.Ohtani, M.Michinomae, Y.Fukada, Y.Shichida and T.Yoshizawa: "Visual pigments in the pineal complex of the Japanese quail, Japanese grass lizard and bullfrog : Immunocytochemistry and HPLC analysis." Tissue & Cell. 26. 101-113 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Okada, T.Matsuda, H.Kandori, Y.Fukada, T.Yoshizawa and Y.Shichida: "Circular dichroism of metaiodopsin II and its binding to transducin : A comparative study between meta II intermadiates of iodopsin and rhodopsin." Biochemistry. 33. 4940-4946 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y.Shichida, H.Imai, y.Imamoto, Y.Fukada and T.Yoshizawa: "Is chicken green-sensitive cone visual pigment a rhodopsin-like pigment? A comparative study of the molecular properties between chicken green and rhodopsin." Biochemistry. 33. 9040-9044 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S.Tamotsu, T.Oishi, K.Nakao, Y.Fukada, Y.Shichida, T.Yoshizawa and Y.Morita: "Localization of iodopsin and rod-opsin immunoreactivity in the retinaand pineal complex of the river lamprey, Lampetra japonica." Cell Tissue Res.278. 1-10 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Okano, T.Yoshizawa and Y.Fukada: "Pinopsin is a chicken pineal photoreceptive molecule." Nature. 372. 94-97 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y.Yoshikawa, Y.Yashiro, T.Oishi, K.Kokame and Y.Fukada: "Immunoreactivities to rhodopsin and rod/cone transducin antisera in the retina, pineal complex and deep brain of the bullfrog, Rana catesbeiana." Zool.Sci.11. 675-680 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Matsuda, T.Takao, Y.Shimonishi, M.Murata, T.Asano, T.Yoshizawa and Y.Fukada: "Characterization of interactions between transducin a/betagamma-subunits and lipid membranes." J.Biol.Chem.269. 30358-30363 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y.Fukada: "Prenylation and carboxylmethylation of G protein gamma subunit. in : "Methods in Enzymology" Vol.250 (P.J.Casey and J.E.Buss, eds) Academic Press" 91-105 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y.Fukada and K.Kokame: "Covalent lipid modifications of heterotrimeric G Proteins." Folia Pharmacol.Jpn.103. 263-272 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Okano and Y.Fukada: "Brain photoreceptor pinopsin and biological clock." Biological Science News. 278. 14-16 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y.Fukada and T.Matsuda: "Biological significance of lipid modifications of G-proteins." Protein, Nucleic Acid and Enzyme. 40. 13-26 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y.Fukada: "Photoreceptor G protein transducin." Experimental Manuals for Bio-Pharmaceuticaal Sciences. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y.Fukada and T.Okano: "Molecular biology of vision." Molecular Biology of Disease. 400-409 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y.Fukada: "Adenylyl cyclase." Manuals for Protein Chemistry. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y.Shichida, T.Okada, H.Kandori, Y.Fukada and T.Yoshizawa: "Nanosecond laser photolysis of iodopsin, a chicken red-sensitive cone visual pigment." Biochemistry. 32. 10832-10838 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.Kokame, Y.Fukada, Y.Shichida, M.Okada, Y.Honda and T.Yoshizawa: "Identification of the alpha-subunits of rod and cone transducin in chicken photoreceptor cells." Exp.Eye Res.57. 135-140 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Asano, R.Morishita, T.Matsuda, Y.Fukada, T.Yoshizawa and K.Kato: "Purification of four forms of the betagamma subunit complex of G proteins containing different gamma subunits." J.Biol.Chem.268. 20515-21519 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.Kameyama, K.Haga, T.Haga, K.Kontani, T.Katada and Y.Fukada: "Activation by G protein betagamma subunits of beta-adrenergic and muscarinic receptor kinase." J.Biol.Chem.268. 7753-7758 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y.Fukada: "Elsevier" Roles of lipids linked to alpha-and gamma-subunits of photoreceptor G protein. in : "Frontiers of Photobiology" (A.Shima et al., eds.). 1993. 195-200

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1997-03-04  

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