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1995 Fiscal Year Final Research Report Summary

Characterization of Anaerobic Petroleum Degrading Bacterium strain HD-1.

Research Project

Project/Area Number 05454037
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 生物・生体工学
Research InstitutionOsaka University

Principal Investigator

IMANAKA Tadayuki  Osaka University, Graduate School of Engineering, Professor, 工学部, 教授 (30029219)

Co-Investigator(Kenkyū-buntansha) MORIKAWA Masaaki  Osaka University, Graduate School of Engineering, Assoc.Professor (20230104)
Project Period (FY) 1993 – 1995
KeywordsPetroleum Degradation / Anaerobic Condition / Bacteria / Tetradecane / 1-Dodecene / Cracking / アルデヒドデカルボニラーゼ
Research Abstract

We isolated a new type of Gram-negative mixotrophic bacterium, Pseudomonas sp. HD-1, which is a facultative anaerobe. Cells were grown on CO_2 as a sole carbon source in the presence of H_2. Anaerobic growth of the strain was enhanced by the addition of aliphatic as well as aromatic hydrocarbons. When cells were grown on n-alkane anaerobically, many oil vacuoes formed in the cells and about 40-50% of the dry cell weight was lipid and oil. The total increase in the hydrophilic components of the cells indicated the anaerobic assimilation of aliphatic hydrocarbon. Electron microscopic observation showed that the cell surface was highly wavy and that the membrane had a multi-layr structure irrespective of the presence of oil.
The strain was demonstrated to degrade tetradecane (C_<14>) under anaerobic condition and one of the major metabolic intermediates was identified as 1-dodecene (C_<12>). This result demonstrates that alkanes are biodegradable without molecular oxygen via a pathway different from beta-oxidation. The strain was found to grow on CO_2 in the presence of tetradecane and absence of H_2 gas and light. These facts strongly indicate that the bacterium is able to utilize alkane as an energy source anaerobically.

  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] 森川正章、今中忠行: "石油を分解する細菌" 化学. 48. 692-693 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 森川正章、今中忠行: "微生物による石油生産" 蛋白質・核酸・酵素. 40. 52-60 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 今中忠行、森川正章: "微生物による石油分解と石油生成" バイオサイエンスとインダストリー. 52. 36-38,898-900 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 森川正章、今中忠行: "CO_2を単一炭素源として生育する石油代謝細菌" 化学と生物. 32. 690-691 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M. Morikawa & T. Imanaka: "Isolation of a uew mixotrophic bacterium which canfix CO_2 and assimilate aliphatic and aromatic hydrocarbons anaerobically" Journal of Fermentation and Bioeugineering. 76. 280-283 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M. Morikawa,M. Kanemoto,and T. Imanaka: "Biological oxidation and cracking of a alkane under anaerobic condition." Journal of Fermentation and Bioengineering. (発表予定).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M.Morikawa & T.Imanaka.: "Isolation of a new mixotrophic bacterium which can fix CO_2 and assimilate aliphatic and aromatic hydrocarbons anaerobically." J.Ferment.Bioeng.76. 280-283 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M.Morikawa & T.Imanaka.: "A petroleum degrading bacterium." CHEMISTRY. 48. 692-693 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Imanaka & M.Morikawa.: "Microbial degradation and synthesis of petroleum." Bioscience & Industry. 52. 898-900 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M.Morikawa & T.Imanaka.: "Petroleum metabolizing bacterium which can grow on CO_2 as a sole carbon source." Kagaku to Seibutsu. 32. 690-691 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M.Morikawa & T.Imanaka.: "Petroleum production by microorganisms." Protein, Nucleic acid and Enzyme. 40. 52-60 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M.Morikawa, M.Kanemoto & T.Imanaka.: "Biological oxidation and cracking of alkane under anaerobic condition." (submitted).

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1997-03-04  

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