1995 Fiscal Year Annual Research Report

アブラナ科自家不和合性の優劣性の発現に関する研究

Research Project

Project/Area Number	05454038
Research Institution	TOHOKU UNIVERSITY
Principal Investigator	日向康吉東北大学, 農学部, 教授 (00005589)
Co-Investigator(Kenkyū-buntansha)	渡辺正夫東北大学, 農学部, 助手 (90240522)
Keywords	アブラナ科植物 / 自家不和合性 / 優性 / 劣性 / S遺伝子 / 自他認識 / SLG / SRK
Research Abstract	アブラナ科自家不和合性は胞子体的に働く1座位のS複対立遺伝子によって説明される。S遺伝子の間では共優性に発現するものが多いが、優性・劣性の関係もある。本研究では、このS遺伝子間の共優性、優劣性の関係の実体を明らかにし、さらに、自家不和合性関連遺伝子を単離して、その優性・劣性の発現機構を分析することを目的としている。まず、日本とトルコのBrassica campestris集団から単離した25のS遺伝子ホモ系統を用いて、S遺伝子間の優劣性について検討した。柱頭側でSヘテロ接合の場合共優性グループと優性グループの2つのグループに分けられるが、花粉側でヘテロ接合の時には共優性グループ、優性グループ、劣性グループの3グループに分かれた。花粉で見られる優劣性と柱頭の優劣性とは関係がなかった。さらに、本研究では、14のS遺伝子組み合わせにおいて、共劣性現象ともいうべき現象が見られた。すなわち、S遺伝子がヘテロ接合となった場合、自家不和合性を示すが、いずれの親とも和合となる現象である。この様な現象は花粉側にのみ見いだされた。以上の優劣性をもとに、いくつかのS遺伝子系統に由来するcDNAライブラリーまたはゲノミックライブラリーから自家不和合性関連遺伝子SLG,SRKの単離・解析を行った。S9系統のSLGとSRKのSドメインとプロモーター領域は、約1.7kbにわたって完全な一致が見られ、この高い相同性が柱頭側の優劣性に関与していると考えられた。また、花粉側で劣性のS29系統からSLGおよびSRBを単離した。これらの間には相互に高い相同性が見られた。

Research Products
(15 results)

All Other

All Publications (15 results)

[Publications] Yamakawa,S.,Watanabe,M.,Isogai,A.,Takayama,S.,Satoh,S.,Hinata,K.and Suzuki,A.: "The cDNA sequence of NS3-glycoprotein from Brassica campestris and its homology to related proteins." Plant Cell Physiol.34. 173-175 (1993)
[Publications] Nou,I.S.,Watanabe,M.,Isuzugawa,K.,Isogai,A.and Hinata,K.: "Isolation of S-allele from a wild population of Brassica campestris L.at Balcesme,Turkey and their characterization by S-glycoprotein." Sex.Plant Reprod.6. 71-78 (1993)
[Publications] Nou,I.S.,Watanabe,M.,Isogai,A.and Hinata,K.: "Comparison of S-alleles and S-glycoproteins between wild populations of Brassica campestris in Turkey and Japan." Sex.Plant Reprod.c. 6. 79-86 (1993)
[Publications] Hinata,K.,Watanabe,M.,Toriyama,K.and Isogai,A.: "A review of recent studies on homomorphic self-incompatibility." Inter.Rev.Cytol.143. 257-296 (1993)
[Publications] Wong,K.C.,Watanabe,M.and Hinata,K.: "Protein profiles in pin and thrum floral organs of distylous Averrhoa carambola L." Sex Plant Reprod.7. 7. 107-115 (1994)
[Publications] Wong,K.C.,Watanabe,M.and Hinata,K.: "Fluorescnese and scanning electron microscopic studies on self-incompatibility in disty lous Averrhoa carambola L." Sex.Plant Reprod.7. 116-121 (1994)
[Publications] Yamakawa,S.,Shiba,H.,Watanabe,M.,Shiozawa H.,Takayama,S.,Hinata,K.,Isogai,A.and Suzuki,A.: "The sequence of S-glycoproteinsinvolved in self-incompatibility of Brassica campestris and their distribution among Brassicaceae." Biosci.Biotech.Biochem.58. 921-925 (1994)
[Publications] Watanabe,M.,Takasaki,T.,Toriyama,K.,Yamakawa,S.,Isogai,A.,Suzuki,A.and Hinata,K.: "A high degree of homology exists between the protein encoded by SLG and the S recept or domain encoded by SRK in self-incompatible Brassica compestris L." Plant Cell Physiol.35. 1221-1229 (1994)
[Publications] Hinata,K.,Watanabe,M.,Isogai A.,Yamakawa,S.,Satta,Y.and Takahata,N.: "Genetic studies of self-incompatible Brassica campestris." “Pollen-pistil interactions and pollen tube growth(Current Topics in Plant Physiology:An American Society of Plant Physiologists Series.). 12. 103-110 (1994)
[Publications] Matsushita,M.,Watanabe,M.,Yamakawa,S.,Isogai,A.and Hinata,K.: "Base sequence variation of the S-glycoprotein with the same S12-allele in Brassica campestris." “Pollen-pistil interactions and pollen tube growth(Current Topics in Plant Physiology:An American Society of Plant Physiologists Series.). 12. 313-315 (1994)
[Publications] Yamakawa,S.,Watanabe,M.,Hinata,K.,Suzuki,A.and Isogai,A.: "The sequences of S-receptor kinase(SRK) involved in self-incompatibility and their homologies to S-locus glycoprotein of Brassica compestris." Biosci.Biotech.Biochem.59. 161-162 (1995)
[Publications] Hinata,K.,Watanabe,M.,Yamakawa,S.,Satta,Y.and Isogai,A.: "Evolutionary aspects of the S-related genes of the Brassica self-incompatibility system:synonymous and nonsynonyomous base substitutions." Genetics. 140. 1099-1104 (1995)
[Publications] Suzuki,G.,Watanabe,M.,Toriyama,K.,Isogai,A.and Hinata,K.: "Molecular cloning of members of the S-multigene family in self-incompatible Brassica campestris L." Plant Cell Physiol.36. 1273-1280 (1995)
[Publications] 渡辺正夫・鳥山欽哉: "花粉学事典(日本花粉学会編,分担執筆)" 朝倉書店, (1994)
[Publications] Hinata,K.,Isogai,A.and Isuzugawa,K.,: "Genetic control of self-incompatibility and reproductive development in flowering plants"eds.by Williams,E.G.,Clarke,A.E.and Knox,R.B.," Kluwer Academic Publishers,102-115 (1994)

1995 Fiscal Year Annual Research Report

アブラナ科自家不和合性の優劣性の発現に関する研究

Principal Investigator

日向 康吉 東北大学, 農学部, 教授 (00005589)

Research Products

[Publications] Yamakawa,S.,Watanabe,M.,Isogai,A.,Takayama,S.,Satoh,S.,Hinata,K.and Suzuki,A.: "The cDNA sequence of NS3-glycoprotein from Brassica campestris and its homology to related proteins." Plant Cell Physiol.34. 173-175 (1993)

[Publications] Nou,I.S.,Watanabe,M.,Isuzugawa,K.,Isogai,A.and Hinata,K.: "Isolation of S-allele from a wild population of Brassica campestris L.at Balcesme,Turkey and their characterization by S-glycoprotein." Sex.Plant Reprod.6. 71-78 (1993)

[Publications] Nou,I.S.,Watanabe,M.,Isogai,A.and Hinata,K.: "Comparison of S-alleles and S-glycoproteins between wild populations of Brassica campestris in Turkey and Japan." Sex.Plant Reprod.c. 6. 79-86 (1993)

[Publications] Hinata,K.,Watanabe,M.,Toriyama,K.and Isogai,A.: "A review of recent studies on homomorphic self-incompatibility." Inter.Rev.Cytol.143. 257-296 (1993)

[Publications] Wong,K.C.,Watanabe,M.and Hinata,K.: "Protein profiles in pin and thrum floral organs of distylous Averrhoa carambola L." Sex Plant Reprod.7. 7. 107-115 (1994)

[Publications] Wong,K.C.,Watanabe,M.and Hinata,K.: "Fluorescnese and scanning electron microscopic studies on self-incompatibility in disty lous Averrhoa carambola L." Sex.Plant Reprod.7. 116-121 (1994)

[Publications] Yamakawa,S.,Shiba,H.,Watanabe,M.,Shiozawa H.,Takayama,S.,Hinata,K.,Isogai,A.and Suzuki,A.: "The sequence of S-glycoproteinsinvolved in self-incompatibility of Brassica campestris and their distribution among Brassicaceae." Biosci.Biotech.Biochem.58. 921-925 (1994)

[Publications] Watanabe,M.,Takasaki,T.,Toriyama,K.,Yamakawa,S.,Isogai,A.,Suzuki,A.and Hinata,K.: "A high degree of homology exists between the protein encoded by SLG and the S recept or domain encoded by SRK in self-incompatible Brassica compestris L." Plant Cell Physiol.35. 1221-1229 (1994)

[Publications] Yamakawa,S.,Watanabe,M.,Hinata,K.,Suzuki,A.and Isogai,A.: "The sequences of S-receptor kinase(SRK) involved in self-incompatibility and their homologies to S-locus glycoprotein of Brassica compestris." Biosci.Biotech.Biochem.59. 161-162 (1995)

[Publications] Hinata,K.,Watanabe,M.,Yamakawa,S.,Satta,Y.and Isogai,A.: "Evolutionary aspects of the S-related genes of the Brassica self-incompatibility system:synonymous and nonsynonyomous base substitutions." Genetics. 140. 1099-1104 (1995)

[Publications] Suzuki,G.,Watanabe,M.,Toriyama,K.,Isogai,A.and Hinata,K.: "Molecular cloning of members of the S-multigene family in self-incompatible Brassica campestris L." Plant Cell Physiol.36. 1273-1280 (1995)

[Publications] 渡辺正夫・鳥山欽哉: "花粉学事典(日本花粉学会編,分担執筆)" 朝倉書店, (1994)

[Publications] Hinata,K.,Isogai,A.and Isuzugawa,K.,: "Genetic control of self-incompatibility and reproductive development in flowering plants"eds.by Williams,E.G.,Clarke,A.E.and Knox,R.B.," Kluwer Academic Publishers,102-115 (1994)

日向康吉東北大学, 農学部, 教授 (00005589)