1995 Fiscal Year Final Research Report Summary
Exploitation of a new method of genetic analysis for quantitative characters in rice using RAPD
| Project/Area Number |
05454040
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Breeding science
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| Research Institution | KYOTO UNIVERSITY |
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Principal Investigator |
TAKATOSHI Tanisaka Kyoto Univ., Fac.of Agric.Associate Professor, 農学部, 助教授 (80026591)
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| Co-Investigator(Kenkyū-buntansha) |
INOUE Hiromo Kyoto Univ., Fac.of Agric.Instructer, 農学部, 助手 (40260616)
NAKAZAKI Tetsuya Kyoto Univ., Fac of Agric.Instructer, 農学部, 助手 (60217693)
OKUMOTO Yutaka Kyoto Univ., Fac.of Agric.Lecturer, 農学部, 講師 (90152438)
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| Project Period (FY) |
1993 – 1995
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| Keywords | rice / quantitative character / RAPD / mutation / gene analysis |
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| Research Abstract |
Out of a large number of heading-time, culm length and glume shape mutants which were induced from Japanese rice varieties by mutagenic treatments or the action of the mutator, 100 wereselected and analyzed for the mutant genes. The results indicated that many of the mutants were controlled by a single mutant gene. Of the detected mutant genes for heading time, the majority of the early-heading genes were recessive, whereas the majority of the late-heading genes were dominant. As for the short culm mutant genes, no dominant genes were detected. The genomic DNAs were extracted from the mutants harboring a single mutant gene by CTAB method, then amplified by PCR technique using about 450 kinds of primers to detect the RAPD (random amplified polymorphic DNA) between the mutants and the original variety. Specific DNA bands were observed in about 30% of the mutants. Each primer rarely produced the specific band : the probability of producing the specific band was estimated to be about 10^<-2>. This suggested that the genetic background of a mutant is much similar to that of the original variety. In the F_2 populations from the crosses of the mutants having the specific bands to their respective original varieties, whether the specific bands cosegregate with the mutant genes or not was examined. Three specific DNA bands proved to cosegreagate with mutant genes, but the other 23 bands seemed not to cosegregate with mutant genes. Considering the results of RFLP analysis practiced for the same materials, these results indicate that RAPD is more effective than RFLP in practicing the genetic analysis of quantitative characters in rice, though the PCR technique should be improved to utilize the RAPD in practical breeding.
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