1994 Fiscal Year Final Research Report Summary
Structure and Regulation of the genecluster for CO_2-fixation from Microorganisms
| Project/Area Number |
05454070
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
応用微生物学・応用生物化学
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| Research Institution | The University of Tokyo |
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Principal Investigator |
KODAMA Tohru The University of Tokyo, Faculty of Agricalture, Proffesor, 農学部, 教授 (30011901)
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| Co-Investigator(Kenkyū-buntansha) |
IGARASHI Yasuo The University of Tokyo, Faculty of Agriculture, Associate Proffesor, 農学部, 助教授 (90114363)
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| Project Period (FY) |
1993 – 1994
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| Keywords | RubisCO / Hydrogen bacteria / Regulation of geme expression / Pseudomonas |
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| Research Abstract |
Pseudomonas hydrogenothermophila and Hydrogenovibrio marinus are the fastest growing CO_2-fixing organisms among all kind of living matters. Both microorganisms fix CO_2 via the Calvin-Benson Cycle. The genetic studies on the CO_2 assimilation by these organisms have been achieved.
RubisCO genes (cbbLS) of Pseudomonas hydrogenothermophila have been cloned and sequenced. We could not find a regulatory gene as cbbR at the upstream of cbbLS,but the existence of 2 open reading flames (cbbQ and cbbO) was confirmed at the downstream of cbbLS.Genetic studies strongly suggested both of CbbQ and CbbO function for the assembly and/or stabilization of CbbLS.
Hydrogenovibrio marinus contains two L8S8-type (CbbLS-1, CbbLS-2) and one L2-type (CbbM) RubisCOs in the cells. The strain seems to adapt natural environment by changing the pattern of the expression of three RubisCOs with different characteristics.
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