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1995 Fiscal Year Final Research Report Summary

STUDIES OF STRUCTURE AND FUNCTION ON CEREBRAL GABA_B RECEPTOR

Research Project

Project/Area Number 05454152
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field General pharmacology
Research InstitutionKYOTO PREFECTURAL UNIVERSITY OF MEDICINE

Principal Investigator

KURIYAMA Kinya  KYOTO PREFECTURAL UNIVERSITY OF MEDICINE,PROFESSOR, 医学部, 教授 (20079734)

Co-Investigator(Kenkyū-buntansha) TSUJIMURA Atusi  KYOTO PREFECTURAL UNIVERSITY OF MEDICINE,ASSISTANT, 医学部, 助手 (50236890)
HIROUCHI Masaaki  KYOTO PREFECTURAL UNIVERSITY OF MEDICINE,ASSISTANT, 医学部, 助手 (70181196)
Project Period (FY) 1993 – 1995
KeywordsGABA_B receptor / G protein-coupled receptor / Purification / Gi protein / Monoclonal antibody
Research Abstract

The GTP-binding protein was purified from the bovine cerebral cortex and this preparation was found to contain the Gi protein but not Gs and Go proteins. It was reconstituted into the phospholipid vesicles with another two functional proteins, purified GABA_B receptor and purified adenylyl cyclase. These reconstitutionexperimentshave indicated that the GABA_B receptor directly inhibits the adenylyl cyclase activity through the Gi protein. On the other hand, a partial amino acid sequence of the GABA_B receptor was analyzed. It has been suggested a possibility that this sequence may be a part of G protein-coupled receptor. Therefore, the polymerase-chain-reaction (PCR) has been attempted using the transmembrane domains (TM), which have highly homologous sequences among various G protein-coupled receptors. A PCR product (278 bp) was obtained and its deduced amino acid sequences indicated that it might be a region from TM III to TM V.Furthermore, a cDNA library from the rat embryo brain was screened using this PCR product as a probe. As the results of these screenings, several positive clones which contained possible coding regions of a novel G protein-coupled receptor were obtained. The size of these cDNA was found to be 1.5-3.0kb.

  • Research Products

    (6 results)

All Other

All Publications (6 results)

  • [Publications] Nakajima K.: "Immunohistochemical demonstration of GABA_B receptors in the rat gastroinstinal tract" Neurochemical Research. 21. 211-215 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kuriyama K.: "Molecular pharmacology of cerebral GABA_B receptor" Pharmacology & Therapeutics. (印刷中).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nakayasu H.: "Diversity of Interacting Receptors (L.G.Abood & A.Lajtha eds.)" New York Academy of Sciences, 12 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nakajima, K.: "Immunohistochemical demonstration of GABA_B receptors in the rat gastrointestinal tract." Neurochem.Res.21. 221-225 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kuriyama, K.: "Molecular pharmacology of cerebral GABA_B receptor." Pharmacol.& Ther.(in press). (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nakayasu, H.: "Diversity of interesting Receptors (Abood, L,G,& Lajtha, A.eds.)" New York Academy of Sciences. 12 (1995)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1997-03-04  

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