• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

1994 Fiscal Year Final Research Report Summary

STUDIES ON EPESTEIN-BARR VIRUS TISSUE DISTRIBUTION AND ITS GENE EXPRESSION

Research Project

Project/Area Number 05454197
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Virology
Research InstitutionHOKKAIDO UNIVERSITY

Principal Investigator

OSATO Toyoro  HOKKAIDO UNIVERSITY,SCHOOL OF MEDICINE,PROFESSOR, 医学部, 教授 (30000912)

Co-Investigator(Kenkyū-buntansha) SUGIURA Makoto  HOKKAIDO UNIVERSITY,SCHOOL OF MEDICINE,INSTRUCTOR, 医学部, 助手 (20241317)
IMAI Shosuke  HOKKAIDO UNIVERSITY,SCHOOL OF MEDICINE,LECTURER, 医学部, 講師 (60232592)
ISHIKURA Hiroshi  HOKKAIDO UNIVERSITY,SCHOOL OF MEDICINE,LECTURER, 医学部, 講師 (70222982)
Project Period (FY) 1993 – 1994
KeywordsEBV / LATENT INFECTION / PCR / GENE EXPRESSION
Research Abstract

Epstein-Barr virus (EBV), a widespread human herpesvirus, establishes a life-long carrier state after primary infection. It is known that EBV infects mainly B lymphocytes and oral epithelia in vivo. However, other potential sites of EBV latent and/or permissive infection in human body have not been fully clarified. To investigate this, systemic autopsied tissues from 18 EBV-seropositiveindividuals without apparent EBV-related dieaseswere examinded for EBV genomic DNA and virus-specific mRNA,by using the polymerase chain reaction technique. EBV DNA was frequently detected in oral mucosa/tongue/salivary gland, esophagus, stomach, lymph node and spleen ; less frequently in bronchi, lung, kidney, adrenal gland, bone marrow and small intestine. In contrast, liver, gall bladder, pancreas, colon, heart muscle and urinary bladder contained no detectable EBV DNA.Reverse transcription-PCR analysis revealed that the latent membrane protein (LMP) 2A gene was expressed in all lymph nodes of the three cases studied, with LMP2B and EBV-determined nuclear antigen (EBNA) 1 transcripts in the lymph node and the LMP2A transcript in the stomach of one case. EBNA2 and LMP1 mRNA were not detected in any of the tissue specimens. The immediate early BZLF1 gene, a key gene for EBV replicative cycle, was also expressed in the lymph nodes, but not in the spleen nor the stomach. These results indicate that EBV preferentially resides in the upper gastrointestinal tract and lympho-hemopoietic tissues where the cells harbor functionally active viral genomes. Moreover, the selective expression of the viral latent infection genes may provide advantages for EBV persistence in the setting of a host immune response. In addition, the localized detection of BZLF1 mRNA suggests that lymph nodes are another possible site of EBV replication in the asymptomatic virus carrier state in vivo.

  • Research Products

    (6 results)

All Other

All Publications (6 results)

  • [Publications] Imai, S., Osato, T.et al: "Gastric carcinoma : Monoclonal epitherial malignant cells expressing Epstein-Barr virus latent infection proteins." Proc.Natl.Acad.Sci.USA. 91. 9131-9135 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Minarovits, J., Osato, T.et al: "Clonality, expression and methylation patterns of the Epstein-Barr virus genomes in lethal midline granulomas classified as peripheral angiocentric T cell lymphomas." J.Gen.Virol.75. 77-84 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Imai, S., Osato, T.et al: "African Burkitt's lymphoma : a plant, Euphorbia tirucalli, reduces Epstein-Barr virus-specific cellular immunity." Anticancer Res.14. 933-6 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tanaka, Y., Osato, T.et al: "Angiocentric immuno-proliferative lesion associated with chronic active Epstein-Barr virus infection in an 11-year-old boy." Am.J.Surg.Pathol.18. 623-31 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Okano, M., Osato, T.et al: "A serological study of Epstein-Barr virus infection in Japanese patients with ataxia telangiectasia." Immunol.Inf.Dis.4. 65-68 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tsutumi, H., Osato, T.et al: "Sequential development of acute meningoencephalitis and transverse myelitis caused by Epstein-Barr virus during infectious mononucleosis." pediatr.Infect.Dis.J.13. 665-7 (1994)

    • Description
      「研究成果報告書概要(欧文)」より

URL: 

Published: 1996-04-15  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi