1994 Fiscal Year Final Research Report Summary
Molecular biological approach for a dignasis and me chanism of sepsis
| Project/Area Number |
05454364
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
General surgery
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| Research Institution | Keio University |
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Principal Investigator |
UEDA Masakazu Keio Univ., Surglry Instructar, 医学部, 助手 (50142419)
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| Co-Investigator(Kenkyū-buntansha) |
KARAHASHI Tsuyoshi Keio Univ., Surglry Instructar, 医学部, 助手 (20245509)
MURAYAMA Yosihiko Keio Univ., Surglry Instructar, 医学部, 助手 (30239553)
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| Project Period (FY) |
1993 – 1994
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| Keywords | Sepsis / Severe Infection Dis. / MRSA / Cytokine on RNA / endothelin / PCR |
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| Research Abstract |
We established a rapid and sensitive diagnostic method for methicillinresistant Staphylococcus aureus (MRSA) bacteremia in postoperative patients using nested polymerase chain reaction.mecA and TSST-1 genes were not detected in the blood samples of any of the 6 normal volunteers, which were negative by blood culture.In all the 12 samples from which MRSA was isolated by blood culture, mecA and TSST-1 genes were detected.In one sample from which S.epidermidis colonies grow after blood culture , mecA was detected but TSST-1 gene was not.Of 28 samples with blood cultures negative for MRSA,2 samples were still positive for mecA and TSST-1 genes.In these 2 patients, MRSA was isolated from specimens other than blood by culture.Although it took at least 48hr to identify MRSA by blood culture method, the presence of mecA and TSST-1 genes was determined by nested PCR method within only 3-4 hr after blood sampling.
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