1994 Fiscal Year Final Research Report Summary
INTRACEREBRAL GRAFTING OF ENGINEERED CELLS
| Project/Area Number |
05454399
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Cerebral neurosurgery
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| Research Institution | OKAYAMA UNIVERSITY |
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Principal Investigator |
OHMOTO Takashi OKAYAMA UNIV.DEPT.OF NEUROL.SURGERY PROFESSOR, 医学部, 教授 (60032900)
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| Co-Investigator(Kenkyū-buntansha) |
DATE Isao OKAYAMA UNIV.DEPT.OF NEUROL.SURGERY STAFF, 医学部・付属病院, 助手 (70236785)
FURUTA Tomohisa OKAYAMA UNIV.DEPT.OF NEUROL.SURGERY LECTURER, 医学部・付属病院, 講師 (30181457)
ASARI Shoji OKAYAMA UNIV.DEPT.OF NEUROL.SURGERY ASSOC.PROF., 医学部, 助教授 (40175857)
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| Project Period (FY) |
1993 – 1994
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| Keywords | gene transfer / neural transplantation / DNA / Parkinson's disease / beta galactosidase |
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| Research Abstract |
We examined the possibility of the gene therapy of neurodegenerative disease such as Parkinson's disese, by continuous inoculation of DNA-liposome complex into the brain.
DNA-liposome complex was formed by mixing the plasmid vector pRSV beta gal containing the E.coli beta galactosidase gene as a reporter with the cationic lipid TRANSFECTAM at the rate of 1 : 3. Five to fifty mu g of DNA was inoculated continuously to the right striatum of 3 to 6 month old male Sprague-Dawley rats by using Osmotic Pump for various periods of time. Four days after the inoculation was completed, the animals were sacrificed and the expression of the gene ws analyzed by X-gal histohemical staining and anti E.coil beta galactosidase immunocytochemical staining. Considerable numbers of stained cells were observed in the limited area around the cannula. The expression of the gene was most intensive in animals with 7 days inoculation, whereas little with 1 day or less with 14 days inoculation. The larger amount of DNA was inoculated, the more intensive expression was obtained, and no obvious cytotoxicity was observed up to 5 mu g of DNA,We suggest this method to be one of the useful options of gene transfer to central nervous system.
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