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1995 Fiscal Year Final Research Report Summary

Clarification of pathophysiologic machanisms of anaphylactic shock and its treatment

Research Project

Project/Area Number 05454426
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Anesthesiology/Resuscitation studies
Research InstitutionJichi Medical School

Principal Investigator

MITSUHATA Hiromasa  Jichi Medical Schol, Lecturer, 医学部, 講師 (70108934)

Co-Investigator(Kenkyū-buntansha) SHIMIZU Reiju  Jichi Medical Schol, Professor, 医学部, 教授 (60010253)
Project Period (FY) 1993 – 1995
KeywordsAnaphylaxis / Nitric Oxide / Cardiac performance / Bronchospasm
Research Abstract

1.We assessed LV diastolic and systolic function in IgE-mediated anaphylaxis in dogs. LV diastolic funciton, i.e., isovolumic relaxation, is little impaired in anaphylaxis, and LV systolic function is relatively well preserved during the carly stage following the onset of anaphylaxis.
2.To verify production of nitric oxide (NO) in anaphylaxis, we measured NO in peripheral tissue in anaphylactic rabbits using an NO-sensitive electrode, which was placed between the superficial abdominal fascia and the rectus abdominis fascia. NO production can be detected in anaphylactic rabbits.
3.We investigated whether a No synthase (NOS) inhibitor improves cardiovascular depression associated with anaphylaxis. After induction of anaphylactic circulatory depression, one group received an NOS inhibitor (Group I,n=6), and the other received saline solution (Group II,n=5). Mean arterial pressure and right atrial pressure were significantly higher in Group I than in Group II.Hematocrit was significantly lower in Group I than in Group II.Cardiac output did not differ between the groups. In conclusion, NOS inhibitor attenuates hypotension, but does not improve cardiac depression in anaphylaxis in dogs.
4.Animals pretreated with L-NAME showed lower survival rates than control animals pretreated with normal saline. The survival rate in L-NAME-pretreated animals was increased by the administration of L-arginine after initiation of anaphylaxis. Cardiac output fell significantly in animals pretreated with L-NAME compared with controls, although venous return was increased. In animals pretreated with L-NAME,pulmonary resistance was significantly increased, and administration of arginine attenuated the bronchospasm. In conclusion, these results, along with the low survival rates in the L-NAME-treated animals, suggest that NO production may be beneficial to cardiac depression and bronchospasm in anaphylaxis in vivo.

  • Research Products

    (10 results)

All Other

All Publications (10 results)

  • [Publications] Mitsuhata H,Saitoh J,Takeuchi H,Hasome H,Horiguchi Y,Simizu R: "An inhibitor of the nitric oxide synthase,N-nitro-L-arginine-methyl ester attenuates hypotension but does not improve cardiac depression in anaphylaxis in dogs." Shock. 3. 447-453 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Mitsuhata H,Saitoh J,Horiguchi Y,Hasome N,Takeuchi H,Shimizu R: "Nitric oxide synthase inhibition is detrimental to cardiac function and promotes bronchospasm in anaphylaxsis in rabbits." Shock. 4. 143-148 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Mitsuhata H,Hasome H,Saitoh J,Takeuchi H,Horiguchi Y,Simizu R,: "Evaluation of left ventricular diastolic function in IgE-mediated anaphylaxis in dogs." Acta Anaesthesiol Scand. 39. 791-796 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Mitsuhata H,Saitoh J,Takeuchi H,Hasome H,Horiguchi Y,Shimizu R: "Production of nitric oxide in anaphylaxis in rabbits." Shock. 2. 381-384 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Mitsuhata H,Shimizu R,Yokoyama MM: "Role of nitric oxide in anaphylaxis." J Clin Immunol. 15. 277-283 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Mitsuhata H,Hasome H,Saitoh J,Takeuchi H,Horiguchi Y,Shimizu R: "Evaluation of left ventricular diastolic function in IgE-mediated anaphylaxis in dogs." Acta Anaesthesiol Scand. 39. 791-796 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Mitsuhata H,Saitoh J,Takeuchi H,Hasome H,Horiguchi Y,Shimizu R: "Production of nitric oxide in anaphylaxis in rabbits." Shock. 2. 381-384 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Mitsuhata H,Saitoh J,Takeuchi H,Hasome H,Horiguchi Y,Shimizu R: "An inhibitor of the nitric oxide synthase, N^<omega>-nitro-L-arginine-methyl ester attenuates hypotension but does not improve cardiac depression in anaphylaxis in dogs." Shock. 3. 447-453 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Mitsuhata H,Saitoh J.Horiguchi Y,Hasome N,Takeuchi H,Shimizu R: "Nitric oxide synthase inhibition is detrimental to cardiac function and promotes bronchospasm in anaphylaxsis in rabbits." Shock. 4. 143-148 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Mitsuhata H,Shimizu R,Yokoyama MM: "Role of nitric oxide in anaphylaxis." J Clin Immunol. 15. 277-283 (1995)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1997-03-04  

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