1994 Fiscal Year Final Research Report Summary
The study of the mecahnism of insulin resistance in polycystic ovarian disease from the view pont of molecular biology.
| Project/Area Number |
05454445
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | MIE UNIVERSITY |
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Principal Investigator |
TOYODA Nagayasu Mie University, Faculty of Medicine, Professor, 医学部, 教授 (40126983)
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| Co-Investigator(Kenkyū-buntansha) |
TABATA Tsutomu Mie University, Faculty of Medicine, Assistant, 医学部, 助手 (40252358)
MINOURA Hiroyuki Mie University, Faculty of Medicine, Assistant, 医学部, 助手 (70242964)
ITO Masazumi Mie University, Hospital, lecturer, 医学部附属病院, 講師 (00159914)
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| Project Period (FY) |
1993 – 1994
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| Keywords | polycystic ovarian disease / insulin resistance / pregnancy / ovulation |
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| Research Abstract |
In order to clarify the pathophysiology of polycystic ovarian disease (PCO), we started the study form the view point of insulin resistance. Lymphocytes were obtained from two cases of PCO which showed moderate hyperinsulinemia and hyperandrogenism, and the DNA sequence of the insulin receptor was determined. However, no abnormal sequences were found in these cases. It was impossible to clarify the further intracellular mechanism of insulin resistance by the present technique, we changed the research to the more basic one on Insulin resistance and sex steroids, and the molecular mechanism of ovulation. On the mechanism of insulin resistance and sex steroids, glucose transporter 4 (GLUT4) mRNA and protein was measured in pregnant rats. Glut 4 mRNA and protein in adipose tissue and skeletal muscle, which were major insulin sensitive tissue, were assayd. Decrease in GLUT4 mRNA and protein in the adipose tissue were observed in the rats near term. On the other hand, molecular mechanism of ovulation was searched by measuring tissue plasminogen activator (TPA) mRNA in the rat ovary. Remarkable changes in TPA mRNA around the ovulation were observed. It suggests the TPA is the one of the very important factors, which were responsible to the process of ovulation. In order to disclose the cause of anovulation in PCO,the further studies combining the both results were in progress.
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