1994 Fiscal Year Final Research Report Summary
ESTABLISHMENT OF A QUANTIFICATION METHOD OF N-MYC ONCOPROTEIN FOR CHILDFOOD TUMOR AND ITS CLINICAL APPLICATION
| Project/Area Number |
05454483
|
|---|
| Research Category |
Grant-in-Aid for General Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
小児外科
|
|---|
| Research Institution | TOHO UNIVERSITY |
|---|
Principal Investigator |
SHIMATAKE Hiroyuki Toho University School of Medicine, Professor & Chairman, 医学部, 教授 (40010110)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
MAKINO Shunichi Jichi Medical College, Associate Professor, 医学部, 助教授 (30157169)
SAJI Tsutomu Toho University School of Medicine, Associate Professor, 医学部, 助教授 (50120275)
INOUE Akira Toho University School of Medicine, Associate Professor, 医学部, 講師 (10151599)
HEMMI Hiromichi Toho University School of Medicine, Associate Professor, 医学部, 助教授 (90165514)
|
|---|
| Project Period (FY) |
1993 – 1994
|
| Keywords | myc oncogenes / N-myc oncogene / Anti N-Myc antibody / Anti pan-Myc antibody / Standwich-type ELISA / Neuroblastoma / Childhood tumor |
|---|
| Research Abstract |
Aberrant expression of human myc gene family members such as c-myc, N-myc, and L-myc occurs in many malignant tumors. Since N-myc gene amplification in neuroblastoma correlates its poor prognosis, amplification is believed to be important as a tumor marker. It is also important to quantitate N-Myc oncoprotein, because there is neuroblastoma of which N-myc gene is not amplified.
In this study, establishment of a quantification method for the gene products of myc gene family, especially N-Myc oncoprotein, and its clinical application were investigated. In first year (1994) supported by this grant, two polyclonal antibodies (anti N-Myc antibody and anti pan-Myc antibody) against N-Myc oncoprotein were prepared by the injection of antigens engineered by genetically. The specificity of these antibodies was studied by Western blot. Anti N-Myc antibody recognizes only N-Myc protein and anti pan-Myc antibody does N-Myc and c-Myc proteins. N-pan-Myc fusion protein was prepared by a similar procedure to that of the antigens and used as a standard protein.
In 1994, using the polyclonal antibodies and the standard protein, conditions for establishing an ELISA (enzyme-linked immunosorbent assay) system and preparation of monoclonal antibody were investigated. Three monoclonal antibodies were obtained by immunization with N-Myc fusion protein. However, these antibodies were not available for ELISA.Standard protein was detectable between 10 to 200 ng per ml. Then the contents of N-myc protein in human neuroblastoma cell lines were measured. Higher amount of the oncoprotein in neuroblastoma cells with amplification or without was detected than in a no N-myc expressing cell line.
Though it is required for an improvement for the ELISA system, it would be able to measure the N-Myc protein contents in clinical samples.
|
-
-
-
-
-
-
-
-
[Publications] Shimatake, H., Shindo, H., Naruki Y., Ohtsuka, S., Nakagawa, C., Hemmi, H.: "Heterogeneous expression of N-myc oncoprotein : Relationship to cell cycle." Clin Chem Enz Commun. 5. 219-225 (1993)
Description
「研究成果報告書概要(欧文)」より
-
[Publications] Tsuchida, Y., Yokomori, K., Taniguchi, F., Hemmi, H., Shimatake, H., Yamamoto, K., and Hanada, R.: "Tumor markers in childhood tumors." Gan-no-Rinsho. 40.
Description
「研究成果報告書概要(欧文)」より
-
-
[Publications] Hemmi, H., Inoue, A., Obana, K., Yamada, K., Hasan, Z., Tsuchida, Y., and Shimatake, H.: "Recent advances in measuring the copy number and the product of N-myc oncogene" Jpn J Pediatr Sug. 27 (in press). (1995)
Description
「研究成果報告書概要(欧文)」より
-
[Publications] Tsuchida, Y., Hemmi, H., Inoue, A., Obana, K., Taniguchi, F., Hayashi, Y., Kanda, N., and Shimatake, H.: "Genetic clinical markers of human neuroblastoma with special reference to N-myc oncogene ; Amplified or not amplified" Tumor Biol. (in press).
Description
「研究成果報告書概要(欧文)」より
-
[Publications] Shimatake, H., Nakagawa, C., Tsukahara, T., and Hemmi, H.: "Storage and active forms of N-myc oncoprotein in mammalian embryos and their role in tumorigenesis and embryogenesis." Recent Advance in Chemistry and Molecular Biology of Cancer Res (Eds.Q.Dai, M.Armor, and Q.Zhena) Springer-Verlag, Heidelberg. 91-96 (1993)
Description
「研究成果報告書概要(欧文)」より
-
[Publications] Shindo, H., Nakagawa, C., Hemmi, H., Ootsuka S., and Shimatake, H.: "Pleiotypic changes in expression of the N-myc gene in neuroblastoma cell lines treated with anti-cancer drugs." Recent Advance in Chemistry and Molecular Biology of Cancer Res (Eds.Q.Dai, M.Armor, and Q.Zhena) Springer-Verlag, Heidelberg. 149-154 (1993)
Description
「研究成果報告書概要(欧文)」より
