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1994 Fiscal Year Final Research Report Summary

Studies on the effect of oxygensensitive enzymes on ecology and acid production of dental plaque.

Research Project

Project/Area Number 05454496
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Functional basic dentistry
Research InstitutionTOHOKU UNIVERSITY

Principal Investigator

YAMADA Tadashi  Tohoku University School of Dentistry, Professor, 歯学部, 教授 (50005021)

Co-Investigator(Kenkyū-buntansha) ABBE Kazuhiko  Tohoku University School of Dentistry, Associate Prof., 歯学部, 助教授 (40151089)
Project Period (FY) 1993 – 1994
KeywordsDental-caries / Anaerobic-conditions / Mutans-streptococci / Anaerobes / Oral-microorganism / Oxygen-sensitive-enzyme / Periodontal-disease / Ribonucleoside-triphosphate-reductase
Research Abstract

Ribonucleoside triphosphate reductase is an only one enzyme that catalyzes the reaction to synthesize deoxyribonucleotides in anaerobic microorganisms. This enzyme is an essential enzyme for growth and survival of these microorganisms, because deoxyribonucleotides are material for DNA synthesis. The activity of this enzyme has been measured with radio isotopes. However, the accurate measurement of the activity was diffcult, because this enzyme is extremely oxygen-sensitive and usage of radioisotopes in an anaerobic glove box is legally difficult especially in Japan. We could first develop a method to estimate the activity of this enzyme by photometric method with diphenylamine as a color producing reagent in the cell-free extracts of Escherichia coli. With this method we then find the activity of this enzyme in an oral microorganism, Streptococcus mutans, a strong cariogenic bacterium. To estimate the activity more accurately, we uesd high performance liquid chromatography. Then, we found that the enzyme in S.mutans is also highly oxygen-sensitive and that this enzyme has a reversible inactive form. The inactive form of this enzyme was activated by a similar way as found in another highly oxygen-sensitive enzyme, pyruvate formate-lyase. It is possible that the same activating enzyme activates these two oxygen-sensitive enzymes. These findings sugggest that the variable oxygen concentration in dental plaque affects strongly the activities of these enzymes and then the microorganisms involved in the initiation and development of dental caries as well as periodontal diseases.

  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] 山田 正: "トゥースフレンドリー協会の検定基準と市場展開" 食品と開発(健康産業新聞社). 29. 11-13 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 山田 正: "歯垢のpH変化と虫歯-トゥースフレンドリー協会の基本的考え方" 月刊フードケミカル. 1994(4). 38-43 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] N.Takahashi: "The role of the succivate pothway on sorbital fermentation by oral Actinomyces viscusus and Actinomyces naeslundii." Oral Microbiol,Immunol. 9. 218-223 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] S.Kalfas: "Initial catabolism sorbital in Actinomyces naeslundii and Actinomyces viscusus." Oral Microbiol,Immunol. 9. 372-375 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] S.Hojo: "Acid profiles and pH of carious dentin in active and arrested Iesions." J.Dent.Res.73. 1853-1857 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 山田 正: "トゥースフレンドリー協会とは" 健康教室. 517. 79-82 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 荒谷 真平: "一般医化学" 南山堂, 380 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 山田 正: "歯科栄養指導" クインテッセンス出版, 150 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Yamada: "The pH change in dental plaque and dental caries." Food Chemicals. 1994-4. 38-43 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] N.Takahashi, S.Kalfas & T.Yamada: "The role of the succinate pathway on sorbitol fermentation by oral Actinomyces viscusus and Actinomyces naeslundii." Oral Microbiol Immunol. 9. 218-223 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S.Hojo, M.Komatus, R.Okuda.N.Takahashi & T.Yamada: "Acid profiles and pH of carious dentin in active and arrested lesions" J Dent Res. 73. 1853-1857 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S.Kalfas, N.Takahashi, & T.Yamada: "Initial catabolism of sorbitol in Actinomyces naeslundii and Actinomyces viscosus." Oral Microbiol Immunol. 9. 372-375 (1994)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1996-04-15  

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