1995 Fiscal Year Final Research Report Summary
Immunological properties of a heat schock protein from Actinobacillus actinomycetemcomitans.
| Project/Area Number |
05454524
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Conservative dentistry
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| Research Institution | Department of Periodontology, School of Dentistry, Aichigakuin University |
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Principal Investigator |
NOGUCHI Toshihide School of dentistry, Aichigakuin University, Professor, 歯学部, 教授 (50014262)
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| Co-Investigator(Kenkyū-buntansha) |
AMANO Katsuhiko School of dentistry, Aichigakuin University, Assistant Lecturer, 歯学部, 講師 (90231117)
FUKUDA Mitsuo School of Dentistry, Aichigakuin University, Assistant professor (40156790)
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| Project Period (FY) |
1993 – 1995
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| Keywords | Periodontitis / Actinobacillus actinomycetemcomitans / Heat shock protein / GroEL-like protein |
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| Research Abstract |
Actinobacillus actinomycelemcomitans is a facultative gram-negative rod that is considered to be associated with human periodontal diseases. Heat shock proteins are a group of proteins with a conserved structure that are synthesized in response to a sudden increase in temperature or to other environmental stress. Among heat shock proteins, the 64-kDa GroEL-like protein has been implicated in the etiology of autoimmune diseases such as rheumatoid arthritis. In this study, we have examined biochemical and immunological properties of a GroEL-like protein from A.actinomycelemcomitans and other periodontopathogenic bacteria.
SDS-polyacrylamide gel electrophoresis of SDS extracts from whole cells of A.actinomycelemcomitans Y4 showed that the shift from 30ﾟC to 42ﾟC or 50ﾟC brought about a significant increase in the relative amount of a 64-kDa protein. Monoclonal antibodies to the 64-kDa protein of A.actinomycelemcomitans cross-reacted with 62 to 64-kDa proteins of A.actinomycelemcomitans serotype a and c.Haemophilus aphrophilus, Haemophilus influenzae. Haemophilus paraphrophilus. and Escherichia coli. Rabbit antisera to whole cells of strain Y4 strongly reacted with the 64-kDa protein of A.actinomycelemcomitans Y4. Western blot analysis showed that alomost no sera from 18 healthy individuals reacted with the 64-kDa protein of A.actinomycelemcomitans Y4.although one serum from one healthy subject did react very weakly with the 64-kDa protein. Both sera from 2 localized juvenile periodontitis patients strongly reacted with the 64-kDa protein. In addition, 6 sera from 19 rapidly progressive periodontitis patients reacted with the 64-kDa protein. These results suggest that the 64-kDa protein of A.actinomycelemcomitans may be an immunodominant antigen of the organism.
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