1994 Fiscal Year Final Research Report Summary
In situ Detection of Virus in Oral Cancer
| Project/Area Number |
05454550
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Surgical dentistry
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| Research Institution | Tsurumi Univ. |
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Principal Investigator |
SATO Junichi Tsurumi University : Dental Medicine, Assistant Professor, 歯学部, 講師 (70187201)
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| Co-Investigator(Kenkyū-buntansha) |
HAMADA Yoshiki Tsurumi University. Dental Medicine, Assistant, 歯学部, 助手 (70247336)
SETO Kanichi Tsurumi University. Dental Medicine, Peofessor, 歯学部, 教授 (60064367)
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| Project Period (FY) |
1993 – 1994
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| Keywords | HPV. / Oral cancer / in situhybridization |
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| Research Abstract |
The sensitivity of human papilloma virus type 16 (HPV-16) DNA detection by DNA in situ hybridization using biotinylated probes (bio-DISH) was estimated by performing this technique on snapfrozen and peraffin embodied tissue of 10 oral squamous cell carcinomas containing increasing amounts of HPV-16 as determined by Southern blot hybridization. We examined the presence of human papillomavirus (HPV) genome in oral squamous cell carcinoma by employing the polymerase chain reaction. we could not detected HPV16DNA by these method in these tissue. But in situhybridization of this section, HPV16 DNA potitive stained carcinoma around cells were present morphologicaly in 2/10 cases.
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