1994 Fiscal Year Final Research Report Summary
Study of receptors, second messengers and DNA binding proteins on noxio usstimulation
| Project/Area Number |
05454551
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Surgical dentistry
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| Research Institution | Osaka Dental University |
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Principal Investigator |
UEDADA Yutaka Osaka Dental University, Dept.of Anesthesilolgy, Professor, 歯学部, 教授 (10067001)
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| Co-Investigator(Kenkyū-buntansha) |
MOMODA Yoshihiro Osaka Dental University, Dept.of Anesthesilolgy, Assistant, 歯学部, 助手 (60247880)
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| Project Period (FY) |
1993 – 1994
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| Keywords | GABA / GABA_A receptor / gamma subunit / Substantia gelatinosa / Glutamate / Primary sensory afferent system / AMPA type receptor / Pain |
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| Research Abstract |
This study aimed at a demonstration for regulation of trigeminal sensory system by amino acids with using some histochemical technics, in situ hybridization histochemistry (ISH), immunohistochemistry and combination method of immunohistochemistry and retrograde tracer technic. We examined the expression of gamma-aminobutyric acid (GABA)_A receptor gamma1 and gamma2 subunit mRNAs in the rat trigeminal ganglion (TG) by ISH.Most TG cells expressed both gamma1 and gamma2 mRNAs simultaneously. Furthermore, expression of the gamma1 subunit in the primary sensory afferents was confirmed at the protein level by immunohistochemistry using antiserum against the gamma1 subunit. These findings indicate that primary sensory afferents receive GABAergic input, probably axo-axonically via GABA_A receptors expressing gamma1 and gamma2 subunits. We demonstrated that a few neurons projecting to the thalamus in the TNC (trigeminal nucleus caudalis) were labeled by antiserum that recognizes GluR1 and GluR2/3 subunits of the AMPA type receptor. However, expression of the gamma1 and gamma2 subunits of the GABA_A receptors indicated that several neurons projecting to the thalamus in the TNC receive GABAergic inputs via a GABA_A receptor which at contains least gamma1 and/or gamma2 subunits.
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