1994 Fiscal Year Final Research Report Summary
An environmental medical study on the induction of metallothionein under stressful conditions
| Project/Area Number |
05454605
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
環境影響評価(含放射線生物学)
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| Research Institution | HOKKAIDO UNIVERSITY |
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Principal Investigator |
KOJIMA Yutaka Hokkaido Univ., Grad. School of Env. Earth Sci., Prof., 大学院・地球環境科学研究科, 教授 (50135555)
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| Co-Investigator(Kenkyū-buntansha) |
KURASAKI Masaaki Hokkaido Univ., Grad. School of Env. Earth Sci., Inst., 大学院・地球環境科学研究科, 助手 (80161727)
NIIOKA Tadashi Hokkaido Univ., Grad. School of Env. Earth Sci., Assoc. Prof., 大学院・地球環境科学研究科, 助教授 (20123953)
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| Project Period (FY) |
1993 – 1994
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| Keywords | metallothionein / metal-binding protein / stress / high-performance-liquid-chromatography / isoforms / noise / immobilization / vibration |
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| Research Abstract |
The goal of this study was firstly to determine whether the synthesis of metallothioneins was induced by various environmental stressors such as noise, vibration, immobilization, or starvation. The goal of the study was secondly to elucidate the role of the proteins on stressful conditions by examining natures of metallothioneins, composition of the isoforms of the proteins, distribution of the proteins in the body, and variations in tissues. The method for the identification and quantification of the isometallothioneins was established by the combination of the bio-chromatography system and ion-exchange chromatography. We demonstrated that the synthesis of metallothioneins was induced and the content of the protein increased in the livers of rats exposed to noise, vibration, immobilization, and starvation.
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