| Co-Investigator(Kenkyū-buntansha) |
HATTA Takashi Research Inst.Technol., Okayama University of Science, Asistant Professor, 技術科学研究所, 講師 (00218497)
TAKIZAWA Noboru Fac.Engineering, Okayama University of Science, Associate Professor, 工学部, 助教授 (50179579)
YAMANKA Kei Fac.Engineering, Okayama University of Science, Professor, 工学部, 教授 (30035951)
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| Research Abstract |
For application of genetically engineered bacteria to cleanup of polluted environments, bacterial genes involved in the degradation of aromatic hydrocarbons (PAHs) and chlorinated armatics were cloned and analyzed. The following results were obtained ; (1) In Pseudomonas putida OUS82 and P.aeruginosa PaK1, all genes encoding enzymes metabolizing naphthalene to salycylate were highly similar to those i the nah operon of plasmid NAH7. Induction of the ability of OUS82 to degrade PAHs was regulated natively, but that of PaK1 was constitutive. While, genes for naphthalene dihydrodiol dehydrogenase in Rhodococcus sp. CIR2 and 3,4-dihydroxyphenanthrene dioxygenase in Alcaligenes faecalis AFK2 were different from the corresponding genes of NAH7. (2) Two enzymes, two components of the dechlorinase and the ring-cleaving dioxygenase, involved in the degradation of 2,4,6-trichlorophenol in P.pickettie DTP0602 were isolated and characterized from the E.coli cells carrying cloned genes, and the fist structures of the proteins were made clear. (3) In the metabolisms of biphenyl, bebzene, toluene, and ethylbenzene in Rhodococcus sp.RHA1, a single dioxygenase was involved in the ring cleavage of thesefour armatics and two different hydrolases mediated the hydrolysis of the ring-fission products.
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