1994 Fiscal Year Final Research Report Summary
Molecular Mechanisms of Spontaneous Mutagenesis Caused by Oxygen Radicals
| Project/Area Number |
05454639
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Molecular biology
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| Research Institution | Nara Institute of Science and Technology (1994)
The University of Tokyo (1993) |
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Principal Investigator |
HISAJI Maki Nara Institute of Science and Technology, バイオサイエンス研究科, 教授 (20199649)
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| Project Period (FY) |
1993 – 1994
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| Keywords | Esherichia coli / Molecular Biology / Mutator / Mismatch repair / DNA replication |
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| Research Abstract |
8-Oxo-dGTP (8-oxo-7,8-dihydrodeoxyguanosine triphosphate) is a potent mutagenic substrate for DNA synthesis, and organisms possess enzymes specifically hydfolyzing 8-oxo-dGTP to the monophosphate form. The accumulatiom of 8-oxo-dGTP in the nucleotide pool induces G : C*T : A transversion as well as A : T*C : G transversion, and Escherichia coli cells possess mechanisms for preventing such mutations. From analyzes of forward mutations induced in cells lacking 8-oxo-dGTPase (MutT protein) and/or repair enzymes that suppress mutations caused by 8-oxoguanine DNA (MutM and MutY proteins), cooperative functions of these proteins in control of the spontaneous mutagenesis became evident. In mutator strains lacking MutT and/or MutM proteins, 8-oxoguanine of DNA increased to a concentration expected from the increased frequency of mutation.
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