1994 Fiscal Year Final Research Report Summary
GENE TRANSFER AND THE EXPRESSION OF A FOREIGNGENE IN VIVO IN POST-MITOTIC NEURONS OF THE ADULT RAT BRAIN
| Project/Area Number |
05454659
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Nerve anatomy/Neuropathology
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| Research Institution | NARA INSTITUTE OF SCIENCE & TECHNOLOGY |
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Principal Investigator |
SHIOSAKA Sadao NARA INSTITUTE OF SCIENCE & TECHNOLOGY PROFESSOR, バイオサイエンス研究科, 教授 (90127233)
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| Project Period (FY) |
1993 – 1994
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| Keywords | Gene transfer / Central nervous system / Neuronal cell / Post-mitotic cells / Mammalian expression vector / 中枢神経系 |
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| Research Abstract |
Neurons in the adult rat brain were transfected in vivo with a simple plasmid that harvored the gene forbeta-galactosidase from Escherichia coli under control of a chiken beta-actin promoter by use of the hemagglutinating virus of Japan (HVJ) and loposomes. Cells that expressed beta-galactosidase were detected only in the target area of the central nervous system for 10 days by light microscopic analysis. Since electron microscopic analysis revealed that the products of the histochemical reaction were predominantly associated with the nuclear membrane and the endoplasmic reticulum of positive cells, it appeard that the products were translated endogenously and had not been entrapped by endocytocis. Furthermore, the products were observed in typical neuronal cells with a large, round, and pale nuclei, and with direct axo-somatic and axo-dendritic synaptic contacts. This report suggests the possibility of introducing functionally sgnificant genes neurons in targeted areas of the adult central nervous system.
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[Publications] Chen, Z-L., Yoshida, S., Kato, K., Momota, Y., Suzuki, J., Tanka, T., Ito, J., Nishino, H., Aimoto, S., Kiyama, H.and Shiosaka, S.: "Expression and activity-dependent changes of a novel limbic-serine protease gene in the hippocampus" J.Neurosci. in press. (1995)
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「研究成果報告書概要(欧文)」より
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[Publications] Yoshida, S., LiN,L-P., Chen, Z-L., Momota, Y., Kato, K., Tanaka, T., Wanaka, T., and Shiosaka, S.: "Basal magnocellular and pontine cholinergic neurons coexpress FGF receptor mRNA" Heurosci. Res.20. 35-42 (1994)
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「研究成果報告書概要(欧文)」より
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[Publications] Takami, K., Kiyota, Y., Iwane, M., Miyamoto, M., Tsukuda, R., Igarashi, K., Shino, A., Wanaka, A., Shiosaka, S.and Tohyama, M.: "Upreguration of fibroblast growth factor-receptor messenger RNA expression in rat following transient forebrain ischemia" Exp/Brain Res.97. 185-194 (1993)
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「研究成果報告書概要(欧文)」より
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[Publications] Imaizumi, K., Iwata, H., Yoshida, S., Sun, G., Okumura, N,and Shiosaka, S.: "Coexistense of amygdaloid b-protein precursor and basic fibroblast growth factor in single cells of the rat parietal cortex, hippocampus and basal magnocellular nucleus" J.Chem.Neuroanat.6. 159-165 (1993)
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「研究成果報告書概要(欧文)」より
