1994 Fiscal Year Final Research Report Summary
DEVELOPMENT OF BIOLOGICAL SPECIMEN PREPARATION TECHNIQUE FOR ATOMIC FORCE MICROSCOPE
| Project/Area Number |
05507002
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| Research Category |
Grant-in-Aid for Developmental Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
Bacteriology (including Mycology)
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| Research Institution | KYUSHU UNIVERSITY |
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Principal Investigator |
AMAKO Kazunobu KYUSHU UNIVERSITY , Fuculty of Medicine, PROFESSOR., 医学部, 教授 (20078752)
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| Co-Investigator(Kenkyū-buntansha) |
FUJITA Koichi NISSEI SANGYO CO., LTD.HEAD, 係長
MORIYA Tetsuhiro KYUSHU UNIVERSITY,Fuculty of Medicine, Research Associate., 医学部, 助手 (10140790)
UMEDA Akiko KYUSHU UNIVERSITY,Fuculty of Medicine, Assistant Professor., 医学部, 講師 (30078604)
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| Project Period (FY) |
1993 – 1994
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| Keywords | AFM / STM / PROBE MICROSCOPE / BIOLOGICAL SPECIMENS / PLASMA COATING TECHNIQUE / PHAGE |
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| Research Abstract |
The most important problem to be solved in the observation of biological specimens by AFM is to stabilize the specimens aginst the scanning force of the probe of the microscope. Without any specific treatment the biological specimens such as phage particles or the bacterial flagella has disappeared from the image after 1st or 2nd scanning. To stabilize the specimen we tried to use the thin membrane of methane or butane. After coating the specimen with these thin membrane we could have good image even after 5-8 scanning with the probe. The thichness of the membrane can be controlled less than 1nm. In such thin membrane we could see the fine structure of bacterial surface layr, the arrangement of protein subunits of S-layr. Our conclusion is that the coating of the membrane is the useful technique for the observation of biological specimens by AFM.
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