1994 Fiscal Year Final Research Report Summary
Development of schistosomiasis vaccines by using squirrel monkeys
| Project/Area Number |
05557018
|
|---|
| Research Category |
Grant-in-Aid for Developmental Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
寄生虫学(含医用動物学)
|
|---|
| Research Institution | The University of Tokyo |
|---|
Principal Investigator |
KOJIMA Somei The University of Tokyo, Institute of Medical Science, Professor, 医科学研究所, 教授 (00009622)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
HATTORI Shosaku The University of Tokyo, Institute of Medical Science, Lecturer, 医科学研究所, 講師 (00164864)
FURUTA Takahisa The University of Tokyo, Institute of Medical Science, Research Assistant, 医科学研究所, 教務職員 (30143514)
MATSUMOTO Naoki The University of Tokyo, Institute of Medical Science, Research Associate, 医科学研究所, 助手 (40239108)
KITA Kiyoshi The University of Tokyo, Institute of Medical Science, Associate Professor, 医科学研究所, 助教授 (90134444)
|
|---|
| Project Period (FY) |
1993 – 1994
|
| Keywords | schistosomiasis japonica / monoclonal IgE / paramyosin / vaccine / squirrel monkey |
|---|
| Research Abstract |
We have demonstrated in previous works that paramyosin is the target molecule of a mouse monoclonal IgE antibody(SJ18epsilon.1)that induces passive immunity against Schistosoma japonicum infection. We used SJ18epsilon.1 for screening of a lambdagt11 library obtained from S.japonicum and finally determined the nucleotide sequence of full length cDNA coding for the target molecule. In the present study, we determined the B cell epitope of paramyosin recognized by SJ18epsilon.1 by using a series of deletion mutants expressed in E.coli and also by using synthetic peptides. The results indicated that the antibody reacts with the part of recombinant paramyosin containing 71 amino acids (Asp^<343>-Ala^<413>). We carried out further mapping of the epitope by using heptameric peptides prepared by multi-pin system and found that the sequence consisting of four amino acids is the epitope of this protective monoclonal antibody. Furthermore, the recombinant paramyosin including this epitope was found to be immunogenic in squirrel monkeys. We also examined if protective immunity to a challenge infection is induced in squirrel monkeys immunized with irradiated cercariae. In immunized monkeys, the egg granuloma formation was small in size as well as in number, while a significant infiltration of neutrophils in the granulomas was observed in control animals with association of severe hemorrhage in surrounding tissues. Thus, the squirrel monkey was considered as a useful vaccine model for schistosomiasis japonica.
|
