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1994 Fiscal Year Final Research Report Summary

Establishment of Molecular Pharmacological assay for Ca^<2+> blocker acting on human brain

Research Project

Project/Area Number 05557119
Research Category

Grant-in-Aid for Developmental Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 応用薬理学・医療系薬学
Research InstitutionKYOTO UNIVERSITY

Principal Investigator

SATOH Masamichi  Kyoto Univ., Faculty of Pharm, Sci, Professor, 薬学部, 教授 (80025709)

Co-Investigator(Kenkyū-buntansha) KANEKO Shuji  Kyoto Univ., Faculty of Pharm, Sci, Associate Professor, 薬学部, 助教授 (60177516)
Project Period (FY) 1993 – 1994
Keywordshuman brain / Ca channel / Ca blocker / Xenopus laevis / oocytes / cDNA / molecular cloning / beta subunit
Research Abstract

1.Complementary DNA for beta subunit of voltage dependent Ca^<2+> channel was cloned from human brain cDNA library and the nucleic acid sequence was determined.
2.Using Xenopus oocytes in which Ca^<2+> channel alpha_1 and beta subunits were coexpressed, practical system for measuring Ca^<2+> channel activity was developed.
3.The beta subunit was nessary for functional expression of Ca^<2+> channels in Xenopus oocytes when they were injected with alpha_1 subunit for neuronal Ca^<2+> channels. Regulatory mechanisms of Ca^<2+> channels by GTP-binding proteins was also clarified.
4.When kappa opioid receptors and Ca^<2+> channels were coexpressed in the oocytes, activity of Ca^<2+> channels was negatively medulated by kappa opioid receptors. Its mechanism was clarified.

  • Research Products

    (5 results)

All Other

All Publications (5 results)

  • [Publications] Kaneko S.et al.: "Ca^<2+>channel inhibition of κ opioid veceptors expressed in Xenopus ooctfes" Neun Reort.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kaneko S.et al.: "Ca^<2+>channel inhibition of κ opioid veceptors expressed in Xenopus ooctfes" Neun Reort. 5.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kaneko S.et al.: "Ca^<2+>channel inhibition of κ opioid veceptors expressed in Xenopus ooctfes" Neun Reort. 5. (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kaneko S.et al.: "Ca^<2+>channel inhibition of κ opioid veceptors expressed in Xenopus ooctfes" Neun Reort. 5. (1994)2506-2508

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kaneko S,et al.: "Ca^<2+> channel inhibition of kappa opioid receptors expressed in Xenopus oocytes" Neuro Report. 5. 2506-2508 (1994)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1996-04-15  

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