1994 Fiscal Year Final Research Report Summary
Establishment of Molecular Pharmacological assay for Ca^<2+> blocker acting on human brain
| Project/Area Number |
05557119
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| Research Category |
Grant-in-Aid for Developmental Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
応用薬理学・医療系薬学
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| Research Institution | KYOTO UNIVERSITY |
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Principal Investigator |
SATOH Masamichi Kyoto Univ., Faculty of Pharm, Sci, Professor, 薬学部, 教授 (80025709)
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| Co-Investigator(Kenkyū-buntansha) |
KANEKO Shuji Kyoto Univ., Faculty of Pharm, Sci, Associate Professor, 薬学部, 助教授 (60177516)
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| Project Period (FY) |
1993 – 1994
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| Keywords | human brain / Ca channel / Ca blocker / Xenopus laevis / oocytes / cDNA / molecular cloning / beta subunit |
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| Research Abstract |
1.Complementary DNA for beta subunit of voltage dependent Ca^<2+> channel was cloned from human brain cDNA library and the nucleic acid sequence was determined.
2.Using Xenopus oocytes in which Ca^<2+> channel alpha_1 and beta subunits were coexpressed, practical system for measuring Ca^<2+> channel activity was developed.
3.The beta subunit was nessary for functional expression of Ca^<2+> channels in Xenopus oocytes when they were injected with alpha_1 subunit for neuronal Ca^<2+> channels. Regulatory mechanisms of Ca^<2+> channels by GTP-binding proteins was also clarified.
4.When kappa opioid receptors and Ca^<2+> channels were coexpressed in the oocytes, activity of Ca^<2+> channels was negatively medulated by kappa opioid receptors. Its mechanism was clarified.
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