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1994 Fiscal Year Final Research Report Summary

Genetic and biochemical analyzes of an accelerated internode overgrowth mutant in rice.

Research Project

Project/Area Number 05660009
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Breeding science
Research InstitutionAichi University of Education

Principal Investigator

KITANO Hidemi  Aichi University of Education, Faculty of Education, Associate Professor, 教育学部, 助教授 (50144184)

Co-Investigator(Kenkyū-buntansha) NAKAMURA Ikuko  Iwate Biotechnology Research Center, Deputy General manager, 遺伝子工学研究部, 主席研究員
Project Period (FY) 1993 – 1994
KeywordsOryza sativa L. / Mutant / Gibberellin / Cell wall / Internode / Shoot growth / cDNA / Uniconazole
Research Abstract

A rice internode overgrowth mutant, awa-odori (AO) was induced from a japonica cultivar, Koshi-hikari by gamma-ray seed irradiation. The slender shoot phenotype alike in seedlings infected by "bakanae" disease caused by Gibberella fujikuroi has been expressed by a recessive gene named ao-1(accelerated internode overgrowth 1). The results of examination on responsibility of mutant seedlings to some combined applications of GA_3 and its retardant, Uniconazole suggested that this characteristics of the mutant is not caused by an over product of endogenous gibberellins as shown in "bakanae" disease.
Double mutant analysis with two cross combinations of AO (ao-1) x Kotake-tamanishiki (d-18) and AO (ao-1) x Tangin-bozu(d-35) also supported this suggestion.
The result of analysis on sugar components of shoot showed that sugar contents of hemicellulose I and cellulose fractions increased significantly in the mutant compared with Koshi-hikari.
Two-dimensional analysis of soluble and cell wall proteins extracted from the basal area of seedling shoot of AO mutant and Koshi-hikari was carried out and some specific protein spots in the mutant were detected from both 2D-PAGEs.
205 sequenced clones in the cDNA library isolated from mRNAs extracted from elongating internodes of AO shoot were researched on the homology to the other nuclertide sequences using DNA-database of Blastn E-mail Server (NCBI) and DNASIS-MAC (Hitachi Software Engineering Co., Ltd.). The result showed that 41 clones among them encoded a partial homology (>40% in the amino acid sequence level) to DNA sequences recorded in the databases.
Further experiment for cloning ao-1 gene is now being continued.

  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] Nakamura,I.: "Nucleofide sequence of a rice cDNA encoding a homolog of the eukaryofic ribsomal rrotein S8." Plant Physiol.(in press).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Chen,W-B.: "Indica-Japonica differentiation in chinese rice landraces." Euphytica. 74. 195-201 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Chen,W-B.: "Distribution of deletion type in cpDNA of cultivated and wild rice." Japan.J.Genet.68. 597-603 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kanno,A.: "Variation in chloroplast DNA from.rice(oryza safiva):differences between deletions mediated by short direct-repeat sequences within a single species." Theor.Appl.Genet.86. 579-584 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kitano,H.: "Hierachical requlation of organ differentiation during embryogenesis in rice." Plant J.3. 607-610 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nakamura,I.: "Accumulation of the products of B-conglycinin α'-subunitgene consistutively expressed in seeds and non-seed tissues of the transgenle pefunia plants." Plant Cell Phsiol.34. 865-872 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 渡邊昭: "植物の形を決める分子機構" 秀潤社, 171 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 蓬原雄三: "育種とバイオサイエンス" 養賢堂, 569 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nakamura I., N.Kameya, T.Aoki, T.Tada, E.Norita, H.Kanzaki and H.Uchimiya: "Nucleotide sequence of a rice cDNA encoding a homology of the eukaryotic rebosomal protein S8." Plant Physiol.(in press).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Chin, W-B., I.Nakamura, Y-I.Sat and N.Nikko: "Indict-Japonica differentiation in Chinese rice laundresses." Euphytica. 74. 195-201 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Chin, W-B., I.Nakamura, Y-I.Sat and N.Nikko: "Distribution of deletion type in cpDNA of cultivated and wild rice." Japan.J.Gent.68. 597-603 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Cowan, A., N.Watanabe, I.Nakamura and A.Hiram.: "Variation in chloroplast DNA from rice (Oryza sativa) : differences between deletions mediated by short direct-repeat sequences within a single species." Theory.Apply.Gent.86. 579-584 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kitano, H., Y.Tumor, H.Swept and Y.Nugget.: "Hierarchical regulation of organ differentiation during embryo genesis in rice." Plant J.3. 607-610 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nakamura, I., P.H.Dupe and R.N.Beach.: "Accumulation of the products of beta-conglycinin alpha'-subunit gene consistutively expressed in seeds and non-seed tissues of the transgenic petunia plants." Plant Cell Physiol.34. 865-872 (1993)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1996-04-15  

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