1995 Fiscal Year Final Research Report Summary
Studies on the breeding of Zingiber species used as condiment vegetables in Japan
| Project/Area Number |
05660037
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
園芸・造園学
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| Research Institution | University of the Ryukyus |
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Principal Investigator |
ADANIYA Shinich University of the Ryukyus, Agriculture, Associate Professor, 農学部, 助教授 (20128466)
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| Project Period (FY) |
1993 – 1995
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| Keywords | Zingiber mioga Roscoe / Zingiber officnale Roscoe / Seed setting / Seed propagation / Breeding |
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| Research Abstract |
Zingiber mioga Roscoe and Z.offcinale Roscoe are sterile crops. In the studies, possibilities in expanding their genetic variations through seed propagation were discussed.
1. Breeding of Zingiber mioga
1) Selection of selfed lines based on morphological characters
One hundred-six selfed lines of the first generation of Z.mioga were raised during 1988 to 1989. From these lines, 29 selfed lines with different number of chromosomes (2n=49-60) were randomly selected together with a parental line and used for analyzing their morphological variations by multivariate analyzes.The analyzes showed that almost all of the selfed lines were smaller than the parent, but three strains among them produced more spikes than the parent. These results show that the breeding of Z.mioga can be improved by selecting desirable genotypes through seed propagation.
2) In vitro mass propagation of Zingiber mioga
It was difficult to induce adventitious buds through the conventional shoot apex culture. Multiple shoots
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were however induced by vertically dividing shoot apex into two pieces and culturing them on agar medium containing 8 or 12 ppm BA.These multiple shoots were continuously propagated by dividing further into small pieces and culturing them on agar medium with the same BA concentrations. Many plantlets could be obtained by shaking the small pieces of multiple shoots in liquid medium with 1 ppm BA.
2. Breeding of Zingiber officinale
1) Pollen fertility of tetraploid ginger
Pollen fertilities(27.3-74.2%)of tetraploid ginger ( 'Kintoki-4x' , 'Sanshu-4x' , 'Philippine-4x' ) were significantly higher than those(0.3-6.2%)of their original diploid cultivars.
2) Environment for pollination of tetraploid ginger
The optimum temperature for pollen germination of the tetraploid ginger ranged from 14ﾟC to 23ﾟC,and pollen tubes in styles were also well grown at 17ﾟC.These results suggest that the optimum environment for pollination of the tetraploid ginger is at 17ﾟC and under saturated humidity. Seed setting under the environment is now under investigation. Less
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