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1994 Fiscal Year Final Research Report Summary

Search for fat-soluble ligand of nuclear orphan receptor.

Research Project

Project/Area Number 05660106
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 応用微生物学・応用生物化学
Research InstitutionTokyo University of Agriculture

Principal Investigator

KATO Shigeaki  Tokyo Univ.of Agriculture, Dep.of Agricultural Chemistry, Associate Professor, 農学部, 助教授 (60204468)

Project Period (FY) 1993 – 1994
Keywordsnuclear receptor / fat-soluble ligand / gene expression / transcriptional control / vitamin D / vitamin A / orphan receptor
Research Abstract

Steroid/thyroid/vitamin A,D nuclear receptors form a gene superfamily, and they resemble each other in terms of structure and function. Besides of the nuclear receptors, whose ligands have been found, receptors with unknown ligands exist as "orphan receptor" . When the ligand for orphan receptor will be identified, a new signaling pathway becomes clear. Moreover, a cross-talk with the signaling pathways for fat-soluble ligands will be clarified.
In the present study, the subtypes and isoforms for vitamin A receptors (RARs, RXRs) and vitamin D receptors (VDR) as well as orphan receptors have been searched with cDNAs encoding RAR,RXR and VDR from the cDNA libraries of various mammalian tissues. Consequently, we found a VDR isoform (VDR1) acting as a dominant negative receptor against the wild type of VDR (VDR0). Considering the presence of multiple active forms of vitamin D on biological actions, one of these compounds may act as a ligand for VDR1. In addition, the functional analysis of identified orphan receptors is under investigation.

  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] H.Sasaki,et al.: "Transcriptional activity of a fluorinated vitamin D analog on VDR-RXR mediated gene expression." Biochemistry. 34. 370-377 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] R.Kojima,et al.: "In vivo isomerization of retinoic acid:rapid isomer exchange and gene expression." J.Biol.Chem.269. 32700-32707 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] A.Nagasaki,et al.: "Vitamin A regulates the expression of apolipoprotein AI and CIII genes in the rats." Biochem.Biopys.Res.Commun.205. 1510-1517 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y.Ohyama,et al.: "Identification of a vitamin D-responsive element in the 5'-flanking region of the rat 25-hydroxy vitamin D3 24-hydroxylase gene." J.Biol.Chem.269. 10545-10550 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H.Mano,et al.: "Positive and negative regulation of retinoid X receptor gene expression by thyroid hormone in the rat." J.Biol.Chem.269. 10545-10550 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M.Okano,et al.: "Retinoic acid up-regulates erythropoietin production in hepatoma cells and vitamin A-depleted rats." FEBS lett.349. 229-233 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 加藤茂明: "核内レセプターと情報伝達" 実験医学バイオサイエンス 羊土社, 1-125(125) (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 加藤茂明: "受容体の分子生物学" 日本臨床 日本臨床社, 17-23(7) (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H.Sasaki, et al.: "Transcriptional activity of a fluorinated vitamin D analog on VDR-RXR mediated gene expression." Biochemistry. 34. 370-377 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] R.Kojima, et al.: "In vivo isomerization of retinoic acid : rapid isomer exchange and gene expression." J.Biol.Chem.269. 32700-32707 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] A.Nagasaki, et al.: "Vitamin A regulates the expression of apolipoprotein AI and CIII genes in the rats." Biochem.Biopys.Res.Commun.205. 1510-1517 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y.Ohyama, et al.: "Identification of a vitamin D-responsive element in the 5'-flanking region of the rat 25-hydroxy vitamin D3 24-hydroxylase gene." J.Biol.Chem. 269. 10545-10550 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H.Mano, et al.: "Positive and negative regulation of retinoid X receptor gene expression by thyroid hormone in the rat." J.Biol.Chem.269. 1591-1594 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M.Okano, et al.: "Retinoic acid up-regulates erythropoietin production in hepatoma cells and vitamin A-depleted rats." FEBS lett.349. 229-233 (1994)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1996-04-15  

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